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• W1965056385 abstract "A staining method is described in which various proteins in polyacrylamide gels can be stained by using eosin Y. After a brief incubation of a polyacrylamide gel in an acidic solution of 1% eosin Y, various proteins, including human erythrocyte membrane sialoglycoproteins which are not detectable by Coomassie blue R-250 (CB), can be detected with a sensitivity of 10 ng protein. This is far more sensitive than CB staining and is comparable to the sensitivity of silver staining. In a Western blot, the antigenicity of an eosin Y stained protein is retained. In addition, proteins on an immunoblot sheet can be detected by eosin Y staining. The method described is rapid, sensitive, and reproducible with various proteins in polyacrylamide gels and has the added advantage of also staining sialoglycoproteins." @default.
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• W1965056385 date "1991-08-01" @default.
• W1965056385 modified "2023-10-06" @default.
• W1965056385 title "Eosin Y staining of proteins in polyacrylamide gels" @default.
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• W1965056385 doi "https://doi.org/10.1016/0003-2697(91)90466-7" @default.
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