FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965092790> ?p ?o ?g. }

• W1965092790 endingPage "e11332" @default.
• W1965092790 startingPage "e11332" @default.
• W1965092790 abstract "Post-translational modifications (PTMs) impact on the stability, cellular location, and function of a protein thereby achieving a greater functional diversity of the proteome. To fully appreciate how PTMs modulate signaling networks, proteome-wide studies are necessary. However, the evaluation of PTMs on a proteome-wide scale has proven to be technically difficult. To facilitate these analyses we have developed a protein microarray-based assay that is capable of profiling PTM activities in complex biological mixtures such as whole-cell extracts and pathological specimens.In our assay, protein microarrays serve as a substrate platform for in vitro enzymatic reactions in which a recombinant ligase, or extracts prepared from whole cells or a pathological specimen is overlaid. The reactions include labeled modifiers (e.g., ubiquitin, SUMO1, or NEDD8), ATP regenerating system, and other required components (depending on the assay) that support the conjugation of the modifier. In this report, we apply this methodology to profile three molecularly complex PTMs (ubiquitylation, SUMOylation, and NEDDylation) using purified ligase enzymes and extracts prepared from cultured cell lines and pathological specimens. We further validate this approach by confirming the in vivo modification of several novel PTM substrates identified by our assay.This methodology offers several advantages over currently used PTM detection methods including ease of use, rapidity, scale, and sample source diversity. Furthermore, by allowing for the intrinsic enzymatic activities of cell populations or pathological states to be directly compared, this methodology could have widespread applications for the study of PTMs in human diseases and has the potential to be directly applied to most, if not all, basic PTM research." @default.
• W1965092790 created "2016-06-24" @default.
• W1965092790 creator A5024750327 @default.
• W1965092790 creator A5042493804 @default.
• W1965092790 creator A5051603473 @default.
• W1965092790 creator A5061810902 @default.
• W1965092790 creator A5084353341 @default.
• W1965092790 creator A5089111286 @default.
• W1965092790 date "2010-06-28" @default.
• W1965092790 modified "2023-10-16" @default.
• W1965092790 title "Development and Validation of a Method for Profiling Post-Translational Modification Activities Using Protein Microarrays" @default.
• W1965092790 cites W101331579 @default.
• W1965092790 cites W1573825647 @default.
• W1965092790 cites W1965581435 @default.
• W1965092790 cites W1981891372 @default.
• W1965092790 cites W1984848300 @default.
• W1965092790 cites W1987868209 @default.
• W1965092790 cites W1989088998 @default.
• W1965092790 cites W1990018583 @default.
• W1965092790 cites W1991189921 @default.
• W1965092790 cites W1994218254 @default.
• W1965092790 cites W2003062513 @default.
• W1965092790 cites W2004704197 @default.
• W1965092790 cites W2006032897 @default.
• W1965092790 cites W2007731694 @default.
• W1965092790 cites W2012165444 @default.
• W1965092790 cites W2020549684 @default.
• W1965092790 cites W2021044341 @default.
• W1965092790 cites W2025450862 @default.
• W1965092790 cites W2029093937 @default.
• W1965092790 cites W2032869951 @default.
• W1965092790 cites W2036644816 @default.
• W1965092790 cites W2041190034 @default.
• W1965092790 cites W2046298465 @default.
• W1965092790 cites W2052291986 @default.
• W1965092790 cites W2054782539 @default.
• W1965092790 cites W2056234356 @default.
• W1965092790 cites W2062600056 @default.
• W1965092790 cites W2068399149 @default.
• W1965092790 cites W2078376874 @default.
• W1965092790 cites W2079238991 @default.
• W1965092790 cites W2082672730 @default.
• W1965092790 cites W2089865526 @default.
• W1965092790 cites W2089980937 @default.
• W1965092790 cites W2091602394 @default.
• W1965092790 cites W2092886089 @default.
• W1965092790 cites W2105247266 @default.
• W1965092790 cites W2106647330 @default.
• W1965092790 cites W2114070867 @default.
• W1965092790 cites W2118929373 @default.
• W1965092790 cites W2121728615 @default.
• W1965092790 cites W2122986822 @default.
• W1965092790 cites W2127248667 @default.
• W1965092790 cites W2131530904 @default.
• W1965092790 cites W2135339359 @default.
• W1965092790 cites W2136128861 @default.
• W1965092790 cites W2144831154 @default.
• W1965092790 cites W2150621953 @default.
• W1965092790 cites W2154637381 @default.
• W1965092790 cites W2157971706 @default.
• W1965092790 cites W2162892143 @default.
• W1965092790 cites W2166835502 @default.
• W1965092790 doi "https://doi.org/10.1371/journal.pone.0011332" @default.
• W1965092790 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2893156" @default.
• W1965092790 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20596523" @default.
• W1965092790 hasPublicationYear "2010" @default.
• W1965092790 type Work @default.
• W1965092790 sameAs 1965092790 @default.
• W1965092790 citedByCount "26" @default.
• W1965092790 countsByYear W19650927902012 @default.
• W1965092790 countsByYear W19650927902013 @default.
• W1965092790 countsByYear W19650927902014 @default.
• W1965092790 countsByYear W19650927902015 @default.
• W1965092790 countsByYear W19650927902016 @default.
• W1965092790 countsByYear W19650927902017 @default.
• W1965092790 countsByYear W19650927902018 @default.
• W1965092790 countsByYear W19650927902019 @default.
• W1965092790 countsByYear W19650927902020 @default.
• W1965092790 countsByYear W19650927902022 @default.
• W1965092790 crossrefType "journal-article" @default.
• W1965092790 hasAuthorship W1965092790A5024750327 @default.
• W1965092790 hasAuthorship W1965092790A5042493804 @default.
• W1965092790 hasAuthorship W1965092790A5051603473 @default.
• W1965092790 hasAuthorship W1965092790A5061810902 @default.
• W1965092790 hasAuthorship W1965092790A5084353341 @default.
• W1965092790 hasAuthorship W1965092790A5089111286 @default.
• W1965092790 hasBestOaLocation W19650927901 @default.
• W1965092790 hasConcept C104317684 @default.
• W1965092790 hasConcept C104397665 @default.
• W1965092790 hasConcept C134459356 @default.
• W1965092790 hasConcept C150194340 @default.
• W1965092790 hasConcept C171350616 @default.
• W1965092790 hasConcept C175114707 @default.
• W1965092790 hasConcept C181199279 @default.
• W1965092790 hasConcept C185592680 @default.
• W1965092790 hasConcept C195814341 @default.
• W1965092790 hasConcept C25602115 @default.
• W1965092790 hasConcept C2779231659 @default.

• next