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- W1965133445 abstract "A simple effective and compact freeze-drying method involving skim milk 20% (w/v) and glutamate 5% or meso-inositol 5% or honey 10% or raffinose 5% for the long-term preservation of bacteria is described. As a case example more than 160 strains representing 36 species of nitrogen-fixing bacteria, 11 species of chemolithorutotrophic bacteria and five species of Aquaspirillum were successfully preserved. All tested strains proved viable and showed about 10–100% survival after freeze-drying and during 2–3 years of storage at +9°C. In such lyophilized cultures no loss in plasmids or other desirable characters was observed. The method is also suitable for the preservation of other fragile and difficult microorganisms as several other strains including bacteria with introduced plasmids could equally survive well and retained plasmids after lyophilization with this method." @default.
- W1965133445 created "2016-06-24" @default.
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- W1965133445 date "1988-09-01" @default.
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- W1965133445 title "A new freeze-drying method for the preservation of nitrogen-fixing and other fragile bacteria" @default.
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- W1965133445 doi "https://doi.org/10.1016/0167-7012(88)90008-5" @default.
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