Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965134412> ?p ?o ?g. }
- W1965134412 endingPage "10" @default.
- W1965134412 startingPage "1" @default.
- W1965134412 abstract "Among aminoaromatics, 2,4-diaminotoluene (2,4-DAT) and 2,6-diaminotoluene (2,6-DAT) represent a conflicting couple of isomers; despite showing the same structural alert to DNA reactivity (and thus potential genotoxicity), they are different in terms of carcinogenicity. Of the two, 2,4-DAT alone is a potent rodent carcinogen, the liver being its major target. According to the literature, assays using various short-term genotoxicity tests have not discriminated satisfactorily between the carcinogenic and non-carcinogenic isomer, both chemicals producing overall positive results. To investigate their mechanism of action, we assayed both 2,4-DAT and 2,6-DAT in F-344 rat liver for their ability to induce DNA adducts, as detected by the 32P-postlabelling technique, and to enhance the induction of preneoplastic foci, as detected by GGT-staining in diethylnitrosamine (DENA)-initiated hepatocytes. Our expectation was that, using the correct target/metabolism, a classic genotoxicity assay and an assay detecting non-genotoxic activities could, together, reflect the different carcinogenic behaviour of the two isomers. The results indicate that, at the single equimolar dose of 250 mg/kg i.p., 2,4-DAT was able to induce ~ 6500 times more DNA adducts than 2,6-DAT; the estimated RAL values for the two isomers were 18.6 × 10−6 and 0.29 × 10−8, respectively. Moreover, of the two, only 2,4-DAT was able to significantly enhance the growth of DENA-initiated hepatocytes. Indeed, liver sections from rats treated with 2,4-DAT (30 daily doses of 25 mg/kg i.g.) exhibited an average total number and area of foci of 10.53/cm2 and 1.22 mm2/cm2 vs. 4.46/cm2 and 0.33 mm2/cm2, for their respective controls. By contrast, no effect on the growth of GGT-positive foci was observed when liver sections from rats treated with 2,6-DAT (30 daily doses of 50 mg/kg i.g.) were scored (5.54 foci per cm2 and total area of 0.42 mm2/cm2). The results indicate that in spite of the structural alert common to the two isomers, 2,4-DAT and 2,6-DAT, only the former appears to significantly affect the carcinogenic process in the liver." @default.
- W1965134412 created "2016-06-24" @default.
- W1965134412 creator A5002615996 @default.
- W1965134412 creator A5024911981 @default.
- W1965134412 creator A5029636114 @default.
- W1965134412 creator A5056483394 @default.
- W1965134412 creator A5087589949 @default.
- W1965134412 date "1995-05-05" @default.
- W1965134412 modified "2023-10-18" @default.
- W1965134412 title "Genotoxic and non-genotoxic activities of 2,4- and 2,6-diaminotoluene, as evaluated in Fischer-344 rat liver" @default.
- W1965134412 cites W1497712600 @default.
- W1965134412 cites W1876374583 @default.
- W1965134412 cites W1966905037 @default.
- W1965134412 cites W1979826300 @default.
- W1965134412 cites W1984811795 @default.
- W1965134412 cites W1987750563 @default.
- W1965134412 cites W1997542915 @default.
- W1965134412 cites W2001580901 @default.
- W1965134412 cites W2002535340 @default.
- W1965134412 cites W2008770449 @default.
- W1965134412 cites W2011795438 @default.
- W1965134412 cites W2020312776 @default.
- W1965134412 cites W2021494550 @default.
- W1965134412 cites W2030132169 @default.
- W1965134412 cites W2032907468 @default.
- W1965134412 cites W2035844786 @default.
- W1965134412 cites W2049866967 @default.
- W1965134412 cites W2050350173 @default.
- W1965134412 cites W2053689309 @default.
- W1965134412 cites W2055628270 @default.
- W1965134412 cites W2066419140 @default.
- W1965134412 cites W2072636839 @default.
- W1965134412 cites W2072891772 @default.
- W1965134412 cites W2072939878 @default.
- W1965134412 cites W2077162937 @default.
- W1965134412 cites W2078150483 @default.
- W1965134412 cites W2079005940 @default.
- W1965134412 cites W2079358955 @default.
- W1965134412 cites W2088055126 @default.
- W1965134412 cites W2088990359 @default.
- W1965134412 cites W2118633304 @default.
- W1965134412 cites W2128442121 @default.
- W1965134412 cites W2130570269 @default.
- W1965134412 cites W2134734608 @default.
- W1965134412 cites W2142233850 @default.
- W1965134412 cites W2147994961 @default.
- W1965134412 cites W2153065586 @default.
- W1965134412 cites W2156490822 @default.
- W1965134412 cites W2402078660 @default.
- W1965134412 cites W2403103251 @default.
- W1965134412 cites W2408823591 @default.
- W1965134412 doi "https://doi.org/10.1016/0300-483x(95)02976-f" @default.
- W1965134412 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7761993" @default.
- W1965134412 hasPublicationYear "1995" @default.
- W1965134412 type Work @default.
- W1965134412 sameAs 1965134412 @default.
- W1965134412 citedByCount "26" @default.
- W1965134412 countsByYear W19651344122012 @default.
- W1965134412 countsByYear W19651344122013 @default.
- W1965134412 countsByYear W19651344122015 @default.
- W1965134412 countsByYear W19651344122019 @default.
- W1965134412 countsByYear W19651344122021 @default.
- W1965134412 countsByYear W19651344122023 @default.
- W1965134412 crossrefType "journal-article" @default.
- W1965134412 hasAuthorship W1965134412A5002615996 @default.
- W1965134412 hasAuthorship W1965134412A5024911981 @default.
- W1965134412 hasAuthorship W1965134412A5029636114 @default.
- W1965134412 hasAuthorship W1965134412A5056483394 @default.
- W1965134412 hasAuthorship W1965134412A5087589949 @default.
- W1965134412 hasConcept C114246631 @default.
- W1965134412 hasConcept C153911025 @default.
- W1965134412 hasConcept C178790620 @default.
- W1965134412 hasConcept C185592680 @default.
- W1965134412 hasConcept C202751555 @default.
- W1965134412 hasConcept C2776120743 @default.
- W1965134412 hasConcept C2778959862 @default.
- W1965134412 hasConcept C2779838395 @default.
- W1965134412 hasConcept C2781247443 @default.
- W1965134412 hasConcept C29730261 @default.
- W1965134412 hasConcept C33070731 @default.
- W1965134412 hasConcept C552990157 @default.
- W1965134412 hasConcept C55493867 @default.
- W1965134412 hasConcept C86803240 @default.
- W1965134412 hasConcept C87644729 @default.
- W1965134412 hasConceptScore W1965134412C114246631 @default.
- W1965134412 hasConceptScore W1965134412C153911025 @default.
- W1965134412 hasConceptScore W1965134412C178790620 @default.
- W1965134412 hasConceptScore W1965134412C185592680 @default.
- W1965134412 hasConceptScore W1965134412C202751555 @default.
- W1965134412 hasConceptScore W1965134412C2776120743 @default.
- W1965134412 hasConceptScore W1965134412C2778959862 @default.
- W1965134412 hasConceptScore W1965134412C2779838395 @default.
- W1965134412 hasConceptScore W1965134412C2781247443 @default.
- W1965134412 hasConceptScore W1965134412C29730261 @default.
- W1965134412 hasConceptScore W1965134412C33070731 @default.
- W1965134412 hasConceptScore W1965134412C552990157 @default.
- W1965134412 hasConceptScore W1965134412C55493867 @default.
- W1965134412 hasConceptScore W1965134412C86803240 @default.