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• W1965146535 abstract "In this study, a recombinant strain Bacillus subtilis WB600 (pMA5) was used to produce Alcaligenes faecalis penicillin G acylase (PGA). The PGA activity and overall PGA productivity were respectively 378 and 7.13 U/l h in a batch culture using a complex medium containing 25 g/l of soluble starch. Fed-batch cultures indicated that glucose had a negative effect on the production of PGA. Starch is a good carbon source, but it could not be directly used as a feed due to high viscosity after heat sterilization. A feed was prepared by limited hydrolysis of starch with a small amount of α-amylase, which greatly improved the fluidity with little formation of glucose. The production of PGA increased to 546 U/l by feeding of the partially hydrolyzed starch. When a mixture containing the partially hydrolyzed starch and Tryptone was added by using a pH-stat strategy, the PGA activity reached 1960 U/l, over five-fold of that obtained in the batch culture, and the overall productivity reached 19.6 U/l h, which was more than two-fold of that in the batch culture." @default.
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• W1965146535 date "2006-08-01" @default.
• W1965146535 modified "2023-09-27" @default.
• W1965146535 title "Production of Alcaligenes faecalis penicillin G acylase in Bacillus subtilis WB600 (pMA5) fed with partially hydrolyzed starch" @default.
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• W1965146535 doi "https://doi.org/10.1016/j.enzmictec.2006.01.011" @default.
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