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- W1965199557 abstract "The study of host–pathogen interactions over past decades has benefited from advances in microscopy and fluorescent imaging techniques. A particularly powerful model in this field is vaccinia virus (VACV), which due to its amenability to genetic manipulation has been a productive model in advancing the understanding of the transport of subcellular cargoes. Conventional light microscopy imposes an upper limit of resolution of ∼250 nm, hence knowledge of events occurring at the sub-viral resolution is based predominantly on studies utilising electron microscopy. The development of super-resolution light microscopy presents the opportunity to bridge the gap between these two technologies. This report describes the analysis of VACV replication using fluorescent recombinant viruses, achieving sub-viral resolution with three-dimensional structured illumination microscopy. This is the first report of successfully resolving poxvirus particle morphologies at the scale of single virus particles using light microscopy." @default.
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- W1965199557 date "2012-12-01" @default.
- W1965199557 modified "2023-09-27" @default.
- W1965199557 title "Sub-viral imaging of vaccinia virus using super-resolution microscopy" @default.
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- W1965199557 doi "https://doi.org/10.1016/j.jviromet.2012.07.003" @default.
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