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- W1965207148 abstract "Abstract Elution behavior of sodium polystyrenesulfonates (NaPSS) on a TSK-GMPW column was discussed. Mobile phases were aqueous solutions of a mixture of Na2HPO4 and NaH2PO4 at pH 8.0 (A), NaH2PO4 (B), and Na2CO3 (C) at various concentrations. At low concentration of monomeric electrolytes in the mobile phase, peaks of NaPSS were sharp and Gaussian and the early elution of NaPSS relative to nonionic polymers was observed. Elution of NaPSS compounds retarded with increasing the concentration of electrolytes in the mobile phase and retained in the column over some value of the concentration. Larger molecular weight NaPSS compounds were apt to retain in the column at lower concentration of electrolytes in the mobile phase. In comparison with chromatograms of NaPSS 6,500 (molecular weight was 6,500) in three different mobile phases, A, B, and C, a hypothesis has been proposed that a factor influences retention volume of NaPSS is not the total ionic strength but the partial ionic strength of Na+. However, the increase the ionic strength to decrease the electrostatic interactions resulted in the increase in the hydrophobic interactions and ideal SEC cannot be achieved in this system. For a low molecular weight compound, sodium-p-toluenesulfonate, a concentration of 0.01 M of a buffer solution was adequate because both electrostatic and hydrophobic interactions were minimized and ideal SEC was achieved." @default.
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- W1965207148 date "1989-04-01" @default.
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- W1965207148 title "Aqueous Size Exclusion Chromatography for Strong Anionic Polyelectrolytes on Hydrophilic Polymer Gel" @default.
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- W1965207148 doi "https://doi.org/10.1080/01483918908049207" @default.
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