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• W1965293182 abstract "A shuttle vector, pZ189, carrying a bacterial suppressor tRNA marker gene ( supF ) was dissolved in Tris-EDTA buffer containing 0.3 M 10 B-enriched boric acid and then irradiated with boron neutron captured beam (BNCB) produced by the nuclear reaction 10 B (n,α) 7 Li with thermal neutrons. A DNA repair-deficient mutant, KS46 ( uvrA − ), of Escherichia coli was transformed with the plasmid DNA, and the transformants carrying mutations on the supF Gene were selected as nalidixic acid-resistant colonies. The mutation frequency (2.4 × 10 −4 ) of pZ189 at the D 10 dose was about 70 times greater than the spontaneous rate (3.5 × 10 −6 ). The plasmid mutations were analyzed using DNA sequencers; 88% of them were base substitutions. A few minus-one frameshifts (7%) and deletions (5%) were detected. Among these base substitutions, transversions of G:C to T:A (42%) and G:C to C:G (29%) predominated. Twenty-seven percent of the base substitutions were G:C to A:T transitions; no A:T to G:C transitions were detected." @default.
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• W1965293182 date "1995-03-01" @default.
• W1965293182 modified "2023-09-23" @default.
• W1965293182 title "Mutations of a shuttle vector plasmid, pZ189, in Escherichia coli induced by boron neutron captured beam (BNCB) containing α-particles" @default.
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• W1965293182 doi "https://doi.org/10.1016/0921-8777(94)00053-9" @default.
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