Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965335747> ?p ?o ?g. }
- W1965335747 endingPage "68" @default.
- W1965335747 startingPage "63" @default.
- W1965335747 abstract "Arginase (EC 3.5.3.1; l-arginine amidinohydrolase) is a key enzyme of the urea cycle that catalyses the conversion of arginine to ornithine and urea, which is the final cytosolic reaction of urea formation in the mammalian liver. The recombinant strain of the yeast Saccharomyces cerevisiae that is capable of overproducing arginase I (rhARG1) from human liver under the control of the efficient copper-inducible promoter CUP1, was constructed. The (His)6-tagged rhARG1 was purified in one step from the cell-free extract of the recombinant strain by metal-affinity chromatography with Ni–NTA agarose. The maximal specific activity of the 40-fold purified enzyme was 1600 μmol min−1 mg−1 protein." @default.
- W1965335747 created "2016-06-24" @default.
- W1965335747 creator A5009739695 @default.
- W1965335747 creator A5032801058 @default.
- W1965335747 creator A5036476639 @default.
- W1965335747 creator A5050711996 @default.
- W1965335747 creator A5061039346 @default.
- W1965335747 creator A5079146279 @default.
- W1965335747 creator A5089177031 @default.
- W1965335747 date "2012-01-01" @default.
- W1965335747 modified "2023-09-26" @default.
- W1965335747 title "Overexpression of (His) 6 -tagged human arginase I in Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography" @default.
- W1965335747 cites W1483456869 @default.
- W1965335747 cites W1502143154 @default.
- W1965335747 cites W1563516740 @default.
- W1965335747 cites W1563904830 @default.
- W1965335747 cites W1965542498 @default.
- W1965335747 cites W1990049690 @default.
- W1965335747 cites W1991620243 @default.
- W1965335747 cites W1996295305 @default.
- W1965335747 cites W2005271042 @default.
- W1965335747 cites W2005570998 @default.
- W1965335747 cites W2038457148 @default.
- W1965335747 cites W2049066732 @default.
- W1965335747 cites W2055948417 @default.
- W1965335747 cites W2057027163 @default.
- W1965335747 cites W2066858504 @default.
- W1965335747 cites W2070543174 @default.
- W1965335747 cites W2070712878 @default.
- W1965335747 cites W2076262316 @default.
- W1965335747 cites W2086053317 @default.
- W1965335747 cites W2100476228 @default.
- W1965335747 cites W2100837269 @default.
- W1965335747 cites W2104338176 @default.
- W1965335747 cites W2109470279 @default.
- W1965335747 cites W2125031388 @default.
- W1965335747 cites W2129431737 @default.
- W1965335747 cites W2133662129 @default.
- W1965335747 cites W2145631328 @default.
- W1965335747 cites W2159157497 @default.
- W1965335747 cites W2162539461 @default.
- W1965335747 cites W4248146972 @default.
- W1965335747 doi "https://doi.org/10.1016/j.pep.2011.09.001" @default.
- W1965335747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21945700" @default.
- W1965335747 hasPublicationYear "2012" @default.
- W1965335747 type Work @default.
- W1965335747 sameAs 1965335747 @default.
- W1965335747 citedByCount "22" @default.
- W1965335747 countsByYear W19653357472013 @default.
- W1965335747 countsByYear W19653357472014 @default.
- W1965335747 countsByYear W19653357472015 @default.
- W1965335747 countsByYear W19653357472016 @default.
- W1965335747 countsByYear W19653357472017 @default.
- W1965335747 countsByYear W19653357472018 @default.
- W1965335747 countsByYear W19653357472019 @default.
- W1965335747 countsByYear W19653357472020 @default.
- W1965335747 countsByYear W19653357472021 @default.
- W1965335747 countsByYear W19653357472022 @default.
- W1965335747 countsByYear W19653357472023 @default.
- W1965335747 crossrefType "journal-article" @default.
- W1965335747 hasAuthorship W1965335747A5009739695 @default.
- W1965335747 hasAuthorship W1965335747A5032801058 @default.
- W1965335747 hasAuthorship W1965335747A5036476639 @default.
- W1965335747 hasAuthorship W1965335747A5050711996 @default.
- W1965335747 hasAuthorship W1965335747A5061039346 @default.
- W1965335747 hasAuthorship W1965335747A5079146279 @default.
- W1965335747 hasAuthorship W1965335747A5089177031 @default.
- W1965335747 hasConcept C104317684 @default.
- W1965335747 hasConcept C153911025 @default.
- W1965335747 hasConcept C164007495 @default.
- W1965335747 hasConcept C179247698 @default.
- W1965335747 hasConcept C181199279 @default.
- W1965335747 hasConcept C185592680 @default.
- W1965335747 hasConcept C198684833 @default.
- W1965335747 hasConcept C2776796294 @default.
- W1965335747 hasConcept C2777468819 @default.
- W1965335747 hasConcept C2777576037 @default.
- W1965335747 hasConcept C2778476535 @default.
- W1965335747 hasConcept C2779222958 @default.
- W1965335747 hasConcept C2780365088 @default.
- W1965335747 hasConcept C40767141 @default.
- W1965335747 hasConcept C43617362 @default.
- W1965335747 hasConcept C515207424 @default.
- W1965335747 hasConcept C55493867 @default.
- W1965335747 hasConcept C77061444 @default.
- W1965335747 hasConcept C86803240 @default.
- W1965335747 hasConceptScore W1965335747C104317684 @default.
- W1965335747 hasConceptScore W1965335747C153911025 @default.
- W1965335747 hasConceptScore W1965335747C164007495 @default.
- W1965335747 hasConceptScore W1965335747C179247698 @default.
- W1965335747 hasConceptScore W1965335747C181199279 @default.
- W1965335747 hasConceptScore W1965335747C185592680 @default.
- W1965335747 hasConceptScore W1965335747C198684833 @default.
- W1965335747 hasConceptScore W1965335747C2776796294 @default.
- W1965335747 hasConceptScore W1965335747C2777468819 @default.
- W1965335747 hasConceptScore W1965335747C2777576037 @default.
- W1965335747 hasConceptScore W1965335747C2778476535 @default.
- W1965335747 hasConceptScore W1965335747C2779222958 @default.