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• W1965373891 abstract "High cell density fed-batch fermentations of Escherichia coli W3110 have been carried out at specific growth rates of less than 0.3 h-1, to investigate the effect of glucose limitation on the physiological state of individual cells. After an initial exponential batch phase, the feed rate was held constant and a final dry cell weight of approximately 50 g per litre was achieved. The fermentations were monitored by mass spectrometry whilst measurements of pH, DOC, CFU/mL, TCN, OD500nm and residual glucose concentrations were made. Satisfactory and reproducible results were obtained. Flow cytometric analysis of cells in broth samples, based on either of two multi-staining protocols, revealed a progressive change in cell physiological state throughout the course of the fermentations. From these measurements it was concluded that the loss in reproductive viability towards the end of the fed-batch process is due to cell death and not due to the formation of a viable but nonculturable state as had previously been reported. Since the presence of a high proportion of dead or dying cells at any time during a fermentation has a detrimental effect on the synthesis of any desired product it is proposed that an on-line flow cytometric analysis and control strategy could be used as a means of increasing overall process efficiency." @default.
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• W1965373891 date "1999-06-20" @default.
• W1965373891 modified "2023-10-08" @default.
• W1965373891 title "Use of multi-staining flow cytometry to characterise the physiological state ofEscherichia coli W3110 in high cell density fed-batch cultures" @default.
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• W1965373891 doi "https://doi.org/10.1002/(sici)1097-0290(19990620)63:6<705::aid-bit8>3.0.co;2-m" @default.
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