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• W1965381503 abstract "Mono-ADP-ribosylation is a post-translational modification of proteins in which the ADP-ribose moiety of NAD is transferred to proteins and is responsible for the toxicity of some bacterial toxins (e.g. cholera toxin and pertussis toxin). NAD:arginine ADP-ribosyltransferases cloned from human and rabbit skeletal muscle and from mouse lymphoma (Yac-1) cells are glycosylphosphatidylinositol-anchored and have similar enzymatic and physical properties; transferases cloned from chicken heterophils and red cells have signal peptides and may be secreted.We report here the cloning and characterization of an ADP-ribosyltransferase (Yac-2), also from Yac-1 lymphoma cells, that differs in properties from the previously identified eukaryotic transferases. The nucleotide and deduced amino acid sequences of the Yac-1 and Yac-2 transferases are 58 and 33% identical, respectively. The Yac-2 protein is membrane-bound but, unlike the Yac-1 enzyme, appears not to be glycosylphosphatidylinositol-anchored. The Yac-1 and Yac-2 enzymes, expressed as glutathione S-transferase fusion proteins in Escherichia coli, were used to compare their ADP-ribosyltransferase and NAD glycohydrolase activities. Using agmatine as the ADP-ribose acceptor, the Yac-1 enzyme was predominantly an ADP-ribosyltransferase, whereas the transferase and NAD glycohydrolase activities of the recombinant Yac-2 protein were equivalent. The deduced amino acid sequence of the Yac-2 transferase contained consensus regions common to several bacterial toxin and mammalian transferases and NAD glycohydrolases, consistent with the hypothesis that there is a common mechanism of NAD binding and catalysis among ADP-ribosyltransferases. Mono-ADP-ribosylation is a post-translational modification of proteins in which the ADP-ribose moiety of NAD is transferred to proteins and is responsible for the toxicity of some bacterial toxins (e.g. cholera toxin and pertussis toxin). NAD:arginine ADP-ribosyltransferases cloned from human and rabbit skeletal muscle and from mouse lymphoma (Yac-1) cells are glycosylphosphatidylinositol-anchored and have similar enzymatic and physical properties; transferases cloned from chicken heterophils and red cells have signal peptides and may be secreted. We report here the cloning and characterization of an ADP-ribosyltransferase (Yac-2), also from Yac-1 lymphoma cells, that differs in properties from the previously identified eukaryotic transferases. The nucleotide and deduced amino acid sequences of the Yac-1 and Yac-2 transferases are 58 and 33% identical, respectively. The Yac-2 protein is membrane-bound but, unlike the Yac-1 enzyme, appears not to be glycosylphosphatidylinositol-anchored. The Yac-1 and Yac-2 enzymes, expressed as glutathione S-transferase fusion proteins in Escherichia coli, were used to compare their ADP-ribosyltransferase and NAD glycohydrolase activities. Using agmatine as the ADP-ribose acceptor, the Yac-1 enzyme was predominantly an ADP-ribosyltransferase, whereas the transferase and NAD glycohydrolase activities of the recombinant Yac-2 protein were equivalent. The deduced amino acid sequence of the Yac-2 transferase contained consensus regions common to several bacterial toxin and mammalian transferases and NAD glycohydrolases, consistent with the hypothesis that there is a common mechanism of NAD binding and catalysis among ADP-ribosyltransferases. Mono-ADP-ribosylation, catalyzed by ADP-ribosyltransferases, involves the transfer of the ADP-ribose moiety of NAD to proteins or free amino acids. ADP-ribosyltransferase activity of some bacterial toxins appears to be involved in the pathogenesis of disease (1Moss J. Vaughan M. Adv. Enzymol. Relat. Areas Mol. Biol. 1988; 61: 303-379PubMed Google Scholar). Cholera toxin ADP-ribosylates an arginine in Gsα, the α-subunit of the stimulatory guanine nucleotide-binding protein, with the resulting activation of adenylyl cyclase and increased intracellular cAMP (1Moss J. Vaughan M. Adv. Enzymol. Relat. Areas Mol. Biol. 1988; 61: 303-379PubMed Google Scholar). Pertussis toxin, on the other hand, modifies a cysteine in the G proteins Gi, Go, and Gt, leading to uncoupling of surface receptors from their downstream effector molecules, thereby affecting adenylyl cyclase activity and ion flux (2Ui M. Moss J. Vaughan M. ADP-ribosylating Toxins and G Proteins: Insights into Signal Transduction. American Society for Microbiology, Washington, D. C.1990: 45Google Scholar). ADP-ribosylation of a modified histidine in eukaryotic elongation factor 2 by diphtheria toxin and Pseudomonas aeruginosa exotoxin A results in the inhibition of protein synthesis, causing cell death (3Wick M.J. Iglewski B.H. Moss J. Vaughan M. ADP-ribosylating Toxins and G Proteins: Insights into Signal Transduction. American Society for Microbiology, Washington, D. C.1990: 31Google Scholar, 4Collier R.J. Moss J. Vaughan M. ADP-ribosylating Toxins and G Proteins: Insights into Signal Transduction. American Society for Microbiology, Washington, D. C.1990: 3Google Scholar). Other toxins use different proteins and, in some instances, different acceptor amino acids as substrates for ADP-ribosylation. ADP-ribosyltransferase activity for which arginine is the acceptor amino acid has been detected in numerous animal tissues. The enzymes have been cloned and characterized from a few species, including rabbit (5Zolkiewska A. Nightingale M.S. Moss J. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 11352-11356Crossref PubMed Scopus (151) Google Scholar) and human (6Okazaki I.J. Zolkiewska A. Nightingale M.S. Moss J. Biochemistry. 1994; 33: 12828-12836Crossref PubMed Scopus (80) Google Scholar) skeletal muscle, chicken heterophils (7Tsuchiya M. Hara N. Yamada K. Osago H. Shimoyama M. J. Biol. Chem. 1994; 269: 27451-27457Abstract Full Text PDF PubMed Google Scholar) and erythroblasts (8Davis T. Shall S. Gene (Amst.). 1995; 164: 371-372Crossref PubMed Scopus (24) Google Scholar), and mouse lymphocytes (9Okazaki I.J. Kim H.-J. McElvaney G. Lesma E. Moss J. Blood. 1996; (in press)PubMed Google Scholar). The skeletal muscle transferases are glycosylphosphatidylinositol (GPI) 1The abbreviations used are: GPIglycosylphosphatidylinositolCTLcytotoxic T lymphocytePIphosphatidylinositolkbkilobasePCRpolymerase chain reactionGSTglutathione S-transferaseHPLChigh pressure liquid chromatography. -linked exoenzymes (5Zolkiewska A. Nightingale M.S. Moss J. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 11352-11356Crossref PubMed Scopus (151) Google Scholar, 6Okazaki I.J. Zolkiewska A. Nightingale M.S. Moss J. Biochemistry. 1994; 33: 12828-12836Crossref PubMed Scopus (80) Google Scholar), which, in cultured mouse skeletal muscle (C2C12) cells, modify the adhesion molecule integrin α7 (10Zolkiewska A. Moss J. J. Biol. Chem. 1993; 268: 25273-25276Abstract Full Text PDF PubMed Google Scholar). ADP-ribosylation of integrin α7 was proposed to play a role in muscle cell development (10Zolkiewska A. Moss J. J. Biol. Chem. 1993; 268: 25273-25276Abstract Full Text PDF PubMed Google Scholar). The GPI-anchored lymphocyte transferase (Yac-1), cloned from the mouse lymphoma (Yac-1) cell line, possesses enzymatic and physical properties similar to those of the rabbit and human skeletal muscle enzymes (9Okazaki I.J. Kim H.-J. McElvaney G. Lesma E. Moss J. Blood. 1996; (in press)PubMed Google Scholar). The heterophil transferase ADP-ribosylates p33, a heterophil granule protein related to the myeloid inhibitor membrane protein Mim-1 (11Mishima K. Terashima M. Obara S. Yamada K. Imai K. Shimoyama M. J. Biochem. (Tokyo). 1991; 110: 388-394Crossref PubMed Scopus (45) Google Scholar, 12Yamada K. Tsuchiya M. Mishima K. Shimoyama M. FEBS Lett. 1992; 311: 203-205Crossref PubMed Scopus (10) Google Scholar). ADP-ribosylation by the chicken transferase of nonmuscle actin results in the inhibition of polymerization (13Terashima M. Mishima K. Yamada K. Tsuchiya M. Wakutani T. Shimoyama M. Eur. J. Biochem. 1992; 204: 305-311Crossref PubMed Scopus (37) Google Scholar). glycosylphosphatidylinositol cytotoxic T lymphocyte phosphatidylinositol kilobase polymerase chain reaction glutathione S-transferase high pressure liquid chromatography. Transferases have been thought to participate in the regulation of mouse cytotoxic T lymphocytes (CTLs). Incubation of CTLs with 10 µM NAD resulted in the ADP-ribosylation of surface proteins and the inhibition of subsequent CTL proliferation. Treatment of CTLs with phosphatidylinositol (PI)-specific phospholipase C, before the addition of NAD, prevents its suppressive effect on CTL proliferation (14Wang J. Nemoto E. Kots A.Y. Kaslow H.R. Dennert G. J. Immunol. 1994; 153: 4048-4058PubMed Google Scholar), consistent with the participation of a GPI-linked ADP-ribosyltransferase. Conceivably, the Yac-1 transferase may be responsible for some of the effects of NAD on lymphocyte function. We describe here the cloning of a second ADP-ribosyltransferase from Yac-1 cells, which has characteristics different from those of the GPI-linked Yac-1 enzyme. This novel ADP-ribosyltransferase (termed Yac-2) is a membrane-associated, but apparently not GPI-anchored, enzyme that possesses significant NAD glycohydrolase activity. Supplies were obtained from the following sources: mouse T cell lymphoma (Yac-1) and rat mammary adenocarcinoma (NMU) cells from American Type Culture Collection (Rockville, MD); Eagle's minimal essential medium with Earle's balanced salt solution containing L-glutamine and Dulbecco's phosphate-buffered saline from BioWhittaker, Inc. (Walkersville, MD); the mouse genomic DNA library in Fix II from Stratagene (La Jolla, CA); the Superscript Lambda system for cDNA synthesis and λ cloning, the Lambda packaging system, and Geneticin (G418) from Life Technologies, Inc.; λ and plasmid DNA isolation maxikits and the Qiaquick gel extraction kit from QIAGEN Inc. (Chatsworth, CA); phosphatidylinositol-specific phospholipase C, β-NAD, and agmatine from Sigma; isopropyl-β-D-thiogalactopyranoside from ICN Biomedicals (Aurora, OH); [carbonyl-14C]NAD (53 mCi/mmol), [adenine-U-14C]NAD (274 mCi/mmol), and [α-32P]dATP (6000 Ci/mmol) from Amersham Corp.; the Random Primed DNA labeling kit from Boehringer Mannheim; Dowex AG 1-X2 from Bio-Rad; and a mouse multitissue Northern blot from CLONTECH (San Diego, CA). A λgt22A cDNA library was generated from poly(A)+ RNA (5 µg) obtained from Yac-1 cells as described previously (9Okazaki I.J. Kim H.-J. McElvaney G. Lesma E. Moss J. Blood. 1996; (in press)PubMed Google Scholar). The λ DNA was packaged and amplified to 2.5 × 1010 plaque-forming units/ml. Rabbit skeletal muscle ADP-ribosyltransferase cDNA (25 ng), labeled with [32P]dATP using the Random Primed DNA labeling kit, was used to screen a mouse genomic library (5 × 104 plaque-forming units). Filters were prehybridized for 4 h in 5 × SSC (1 × SSC = 0.15 M NaCl, 15 mM sodium citrate), 1 × Denhardt's solution (0.02% bovine serum albumin, 0.02% polyvinylpyrrolidone, and 0.02% Ficoll), 50% formamide, 10% dextran sulfate, 0.5% SDS, and 100 µg/ml salmon sperm DNA and hybridized overnight in the same solution containing the labeled rabbit muscle transferase cDNA. Filters were washed once in 2 × SSC and 0.1% SDS at 25°C for 20 min and twice in 1 × SSC and 0.1% SDS at 42°C. λ DNA from a single purified clone was isolated using the λ DNA isolation maxikit. λ DNA (5 µg) was digested with BamHI restriction endonuclease (Boehringer Mannheim), size-fractionated on a 1% agarose gel, and transferred to a Nytran membrane using the Turboblotter transfer system (Schleicher & Schuell). The membrane was prehybridized and hybridized as described above and washed three times in 2 × SSC and 0.1% SDS at 25°C for 10 min and twice in 0.1 × SSC and 0.1% SDS at 50°C. XAR film (Eastman Kodak Co.) was exposed to the membrane for 18 h. A 6-kb DNA fragment that hybridized with the rabbit muscle transferase was subcloned into the pGEM7Z- vector (Promega, Madison, WI); competent E. coli cells were transformed with the plasmid containing the 6-kb insert and grown on LB plates containing ampicillin (100 µg/ml). Recombinant plasmid DNA was isolated from E. coli cells using the QIAGEN maxikit. The DNA insert, sequenced using the 7-deaza-dGTP sequencing kit (U. S. Biochemical Corp.), had a 450-base pair open reading frame with a deduced amino acid sequence that was ~30% identical to those of the rabbit muscle and Yac-1 transferases. The 450-base pair DNA was amplified from the plasmid DNA by polymerase chain reaction (PCR) using the upstream (5′-TTTGATGATGCCTATGTGGGCTGC-3′) and downstream (5′-TGGGGGTATCAGCACCTCACGCTC-3′) primers (1 µM each), 10 ng of plasmid DNA, and the PCR Master kit (Boehringer Mannheim), which contains premixed deoxynucleotides and Taq polymerase, for 30 cycles of 95°C for 1 min, 55°C for 1 min, and 72°C for 1.5 min, followed by a 7-min extension at 72°C. The PCR product, purified from a 1% agarose gel with the Qiaquick gel extraction kit, was labeled with [32P]dATP and used to screen the Yac-1 cDNA library (5 × 104 plaque-forming units). Filters were prehybridized, hybridized, and washed as described for the mouse genomic library. λ DNA from a single clone was isolated, and the cDNA insert was amplified from the phage DNA (100 ng) by PCR with forward (5′-CUACUACUACUAGGTGGCGACGACTCCTGGAGCC-3′) and reverse (5′-CAUCAUCAUCAUGACACCAGACCAACTGGTAATG-3′) primers (100 pmol each) using the PCR Master kit under conditions identical to those described above. The 1.5-kb PCR product was gel-purified and subcloned into a pAMP1 vector using the CLONEAMP system (Life Technologies, Inc.). The plasmid was isolated from E. coli, and both strands of the cDNA insert were sequenced. A multitissue Northern blot containing poly(A)+ RNA (2 µg) from mouse tissues was prehybridized at 42°C for 4 h in 5 × SSPE (1 × SSPE = 0.15 M NaCl, 10 mM NaH2PO4, and 1 mM Na2EDTA, pH 7.4), 10 × Denhardt's solution, 50% formamide, 2% SDS, and 100 µg/ml salmon sperm DNA, followed by hybridization overnight at 42°C in the same solution containing a [32P]dATP-labeled Yac-2 transferase cDNA probe. Membranes were washed three times in 2 × SSC and 0.05% SDS at 25°C for 10 min and twice in 0.5 × SSC and 0.1% SDS at 42°C for 20 min. Film was exposed to the membrane for 48 h. cDNAs for truncated forms of the Yac-1 and Yac-2 transferases lacking the amino- and carboxyl-terminal hydrophobic sequences were generated by PCR and used to express the transferases as GST fusion proteins in E. coli cells. For Yac-1, 23 and 37 amino acids were deleted from the N and C termini, respectively, by PCR using forward (5′-ACGACGACGCCGCGGAGTTACTCCATCTCACAACTA-3′) and reverse (5′-ACGTACGTACGTCCGCGGTCAACCCAGCCAGCAGGGCCCAGA-3′) primers (100 pmol each), Yac-1 phage DNA (100 ng), and the PCR Master kit under conditions identical to those described above. For Yac-2, 16 N-terminal and 26 C-terminal amino acids were deleted by PCR using forward (5′-ACGTACCCGCGGGCCCTCTGGAAGGTTCGAGCTGTT-3′) and reverse (3′-ACGTACCCGCGGGGAGGGTGCTCTTGGCTGCCCGAC-3′) primers, Yac-2 phage DNA, and the PCR Master kit as described above. The truncated Yac-1 and Yac-2 PCR products contained KpnI restriction enzyme sites at their 5′- and 3′-ends for subcloning into the KpnI site of the pGEX5GLIC vector (15Haun R. Moss J. Gene (Amst.). 1992; 112: 37-43Crossref PubMed Scopus (42) Google Scholar). After confirming proper orientation of the cDNA inserts, E. coli (DH5α) cells were transformed with the plasmids, and protein expression was induced as described (15Haun R. Moss J. Gene (Amst.). 1992; 112: 37-43Crossref PubMed Scopus (42) Google Scholar). Briefly, transformed bacteria grown at 37°C to an A600 of 0.4 in 1 liter of LB medium containing ampicillin (100 µg/ml) were induced with 0.3 mM isopropyl-β-D-thiogalactopyranoside and incubated at 37°C for 3 h. Following induction, cells were suspended in 15 ml of Dulbecco's phosphate-buffered saline and incubated for 30 min on ice with 5 mg of lysozyme. After sonification for 1 min, Triton X-100 was added (1% final concentration), and the lysate was centrifuged (5000 × g, 20 min). Solubilized GST fusion proteins, purified according to the manufacturer's protocol using glutathione-Sepharose 4B (Pharmacia Biotech Inc.), were assayed for ADP-ribosyltransferase and NAD glycohydrolase activities. Yac-2 transferase cDNA was subcloned into a pMAMneo expression vector as described previously (6Okazaki I.J. Zolkiewska A. Nightingale M.S. Moss J. Biochemistry. 1994; 33: 12828-12836Crossref PubMed Scopus (80) Google Scholar). The Yac-2 cDNA was amplified from purified phage DNA by PCR using forward (5′-ACGTACGTACGTGCTAGCATGATTCTGGAGGATCTGCTGATG-3′) and reverse (5′-ACGTACGTACGTCTCGAGTCAGGGTCCAGCTCTGGAGAGCTG-3′) primers (100 pmol each) under conditions identical to those described above. The PCR product was gel-purified and subcloned into the NheI (5′) and XhoI (3′) sites of the pMAMneo vector. NMU cells were transfected with 15 µg of the purified pMAMneo vector by the calcium phosphate precipitation method. Transformed NMU cells were selected with G418 (500 µg/ml). Expression of ADP-ribosyltransferase was induced by incubating 1 × 106 transformed cells with 1 µM dexamethasone sodium phosphate (MG Scientific, Buffalo Grove, IL) for 24 h. Cells were washed and incubated with or without 0.1 unit of PI-specific phospholipase C in 0.7 ml of Dulbecco's phosphate-buffered saline at 37°C for 1 h, and the medium was collected. Trypsinized cells were lysed in 0.7 ml of hypotonic lysis buffer (10 mM Tris, pH 8.0, and 1 mM EDTA), followed by centrifugation (100,000 × g) for 1 h. The supernatant (0.7 ml) was collected, and the membrane fraction was suspended in 0.7 ml of lysis buffer. ADP-ribosyltransferase or NAD glycohydrolase activity was determined in samples (50 µl) of the medium, supernatant, and membranes. Data are expressed as total activity/fraction (pmol/min [14C]ADP-ribosylagmatine formed in transferase assays or [14C]nicotinamide released in NAD glycohydrolase assays). The ADP-ribosyltransferase reaction was carried out in 0.3 ml containing 50 mM potassium phosphate, pH 7.5, 20 mM agmatine, and 0.1 mM [adenine-U-14C]NAD (0.05 µCi). After incubation at 30°C, duplicate samples (100 µl) were applied to 1-ml columns of Dowex AG 1-X2. [14C]ADP-ribosylagmatine was eluted for radioassay with 5 ml of H2O. The NAD glycohydrolase assay was carried out in 50 mM potassium phosphate, pH 7.5, without and with 20 mM agmatine and 0.1 mM [carbonyl-14C]NAD (0.05 µCi) in a total volume of 0.3 ml. After incubation at 30°C for 1 h, samples (100 µl) were applied to 1-ml columns of Dowex AG 1-X2; [14C]nicotinamide was eluted for radioassay with 5 ml of H2O. The 450-base pair DNA amplified from the genomic library was used as a probe to clone the full-length cDNA from a Yac-1 cDNA library. This clone has an open reading frame of 927 nucleotides, coding for a protein of 309 amino acids (Fig. 1). The 5′-untranslated region contains four in-frame stop codons at positions -348, -324, -117, and -96; the 3′-untranslated region has a stop codon at positions 928-930 and a polyadenylation signal (AATTAAA) at positions 1142-1148, followed by a poly(A)+ tail. The hydrophobicity profiles of the deduced amino acid sequences of the Yac-2 and Yac-1 transferases demonstrate hydrophobic amino termini (data not shown). In contrast, the Yac-1, but not the Yac-2, transferase contained a hydrophobic signal sequence at the carboxyl-terminal end, characteristic of GPI-anchored proteins. The nucleotide and deduced amino acid sequences of the Yac-1 and Yac-2 proteins were 58 and 33% identical, respectively. Comparison of amino acids 38-289 of the Yac-1 transferase and amino acids 28-273 of the Yac-2 transferase, which excludes the amino- and carboxyl-terminal residues, reveals 40% sequence identity (Fig. 2). Whereas the nucleotide and deduced amino acid sequences of the Yac-1 and rabbit muscle transferases were both 75% identical, the nucleotide and amino acid sequences of Yac-2 were 59 and 30% identical, respectively, to those of the muscle enzyme. Furthermore, the deduced amino acid sequence of the Yac-2 transferase is ~28% identical to those of the rat RT6.1 and RT6.2 and mouse Rt6 locus 1 (Rt6-1) T cell alloantigens (Fig. 2), which possess NAD glycohydrolase and, in some instances, ADP-ribosyltransferase activities (16Takada T. Iida K. Moss J. J. Biol. Chem. 1994; 269: 9420-9423Abstract Full Text PDF PubMed Google Scholar, 17Haag F. Andresen V. Karsten S. Koch-Nolte F. Thiele H.-G. Eur. J. Immunol. 1995; 25: 2355-2361Crossref PubMed Scopus (68) Google Scholar, 18Rigby M. Bortell R. Stevens L.A. Moss J. Kanaitsuka T. Shigeta H. Mordes J.P. Greiner D.L. Rossini A.A. J. Immunol. 1996; 156: 4259-4265PubMed Google Scholar, 19Maehama T. Nishina H. Hoshino S. Kanaho Y. Katada T. J. Biol. Chem. 1995; 270: 22747-22751Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar, 20Koch-Nolte F. Petersen D. Balasubramanian S. Haag F. Kahlke D. Willer T. Kastelein R. Bazan F. Thiele H.-G. J. Biol. Chem. 1996; 271: 7686-7693Abstract Full Text Full Text PDF PubMed Scopus (98) Google Scholar, 21Takada T. Iida K. Moss J. J. Biol. Chem. 1995; 270: 541-544Abstract Full Text Full Text PDF PubMed Scopus (59) Google Scholar). Highly conserved regions are evident, suggesting that these enzymes may share similar mechanisms of NAD binding and catalysis (20Koch-Nolte F. Petersen D. Balasubramanian S. Haag F. Kahlke D. Willer T. Kastelein R. Bazan F. Thiele H.-G. J. Biol. Chem. 1996; 271: 7686-7693Abstract Full Text Full Text PDF PubMed Scopus (98) Google Scholar, 21Takada T. Iida K. Moss J. J. Biol. Chem. 1995; 270: 541-544Abstract Full Text Full Text PDF PubMed Scopus (59) Google Scholar). On Northern analysis using poly(A)+ RNA, the Yac-2 cDNA hybridized strongly with 1.6- and 2.0-kb bands from mouse testis (Fig. 3) and weakly with a 1.6-kb band from mouse skeletal and cardiac muscle. In addition, a Yac-2-specific oligonucleotide primer corresponding to amino acids 2-17 hybridized with a 1.6-kb band in poly(A)+ RNA from mouse skeletal muscle and rat testis (data not shown). The Yac-1 transferase cDNA hybridized on Northern blotting with poly(A)+ RNA from mouse cardiac and skeletal muscle, but not with that from testis (9Okazaki I.J. Kim H.-J. McElvaney G. Lesma E. Moss J. Blood. 1996; (in press)PubMed Google Scholar). NMU cells transformed with the Yac-2 cDNA demonstrated ADP-ribosyltransferase activity using agmatine as an ADP-ribose acceptor (data not shown). Activity was found in the membrane fraction of cell lysates, as it was in cells transformed with the Yac-1 cDNA (9Okazaki I.J. Kim H.-J. McElvaney G. Lesma E. Moss J. Blood. 1996; (in press)PubMed Google Scholar). There was negligible transferase or NAD glycohydrolase activity in cells transformed with the pMAMneo vector alone (data not shown). Yac-2 enzyme activity, unlike that of Yac-1, was not released from the membrane with PI-specific phospholipase C. Whereas the Yac-1 transferase was solubilized by PI-specific phospholipase C in a concentration-dependent manner from the intact Yac-1-transformed cells, Yac-2 activity was unaffected by as much as 1.0 unit of PI-specific phospholipase C (Table I).TABLE I.PI-specific phospholipase C-catalyzed release of ADP-ribosyltransferase activity from NMU cells transformed with Yac-1, but not Yac-2, transferase cDNAADP-ribosyltransferase activityYac-1Yac-2PBSaPBS, phosphate-buffered saline; Sup, supernatant; Mem, membranes; PLC, phospholipase C; ND, not detectable.SupMemPBSSupMemnmol/min-1pmol/min-1PLC0 unit0.0310.6851.27NDND14.00.01 unit0.0450.7641.390.181ND15.90.1 unit0.2200.5611.13NDND13.91.0 unit1.120.4620.19NDND14.8a PBS, phosphate-buffered saline; Sup, supernatant; Mem, membranes; PLC, phospholipase C; ND, not detectable. Open table in a new tab The Yac-1 and Yac-2 transferases, expressed in E. coli cells as GST fusion proteins, were used to compare ADP-ribosyltransferase and NAD glycohydrolase activities of the purified enzymes. The GST fusion proteins, purified using glutathione-Sepharose 4B, were ~60% pure on SDS-polyacrylamide gel electrophoresis using a 10% gel (data not shown) stained with 2% Coomassie Brilliant Blue (Bio-Rad). The transferase and NAD glycohydrolase activities of the Yac-1 and Yac-2 enzymes are shown in Table II. The ADP-ribosyltransferase activity of the Yac-1 enzyme was twice that of the recombinant Yac-2 protein, while the NAD glycohydrolase activity of Yac-1 was minimal. The transferase and NAD glycohydrolase activities of the Yac-2 enzyme, on the other hand, were approximately equal. For both enzymes, ADP-ribosylation was agmatine-specific. As determined by Lineweaver-Burk analysis, the Km values (means ± S.E., n = 4) for NAD (1-1000 µM) with 20 mM agmatine as the ADP-ribose acceptor in the ADP-ribosyltransferase assay were 118 ± 17 and 142 ± 13 µM for Yac-1 and Yac-2, respectively; the values for agmatine in the presence of 0.1 mM NAD were 9.4 ± 1.7 and 15 ± 4.9 mM, respectively. In these experiments, <5% of the NAD was utilized. Vmax values in the presence of 20 mM agmatine for the Yac-1 and Yac-2 transferases were 19 ± 5 and 8 ± 3 pmol min-1µg-1, respectively.TABLE II.ADP-ribosyltransferase and NAD glycohydrolase activities of Yac-1- and Yac-2-GST fusion proteinsADP-ribosylagmatine formationNicotinamide releasepmol min-1Yac-1+AgmatineaAgmatine was at 20 mM.11.5 ± 1.811.1 ± 2.0-Agmatine0.45 ± 0.250.86 ± 0.47Yac-2+Agmatine6.2 ± 1.215.4 ± 1.6-Agmatine0.57 ± 0.377.9 ± 1.6a Agmatine was at 20 mM. Open table in a new tab To confirm ADP-ribosylation of guanidino compounds by the Yac-2 transferase, the recombinant Yac-2 protein was incubated with NAD and [14C]arginine, and the reaction products were analyzed by HPLC. As shown in Fig. 4, the Yac-2 transferase generated ADP-ribose-[14C]arginine, consistent with the fact that the Yac-2 protein is a NAD:arginine ADP-ribosyltransferase. The Yac-2 enzyme cloned from Yac-1 lymphoma cells is an apparently unique member of the mammalian ADP-ribosyltransferase family. Although the Yac-2 transferase is a membrane-associated protein, it does not appear to be GPI-linked as is the Yac-1 enzyme. Although both proteins are NAD:arginine ADP-ribosyltransferases, the Yac-2 enzyme has significant basal NAD glycohydrolase activity. This may reflect the fact that agmatine was used as the model substrate in vitro, although for Yac-2, the Km value for agmatine was only ~1.5 times that for the Yac-1 transferase. In the presence of an ideal substrate in vivo, the transferase activity of the Yac-2 protein may be more pronounced. In contrast to Yac-1, the Yac-2 gene is expressed in testis. The presence on Northern analysis of a 1.6- and 2.0-kb doublet in poly(A)+ RNA from testis may be the result of alternative splicing of the Yac-2 transferase mRNA, differential use of alternative polyadenylation signals (22Mishima K. Price S.R. Nightingale M.S. Kousvelari E. Moss J. Vaughan M. J. Biol. Chem. 1992; 267: 24109-24116Abstract Full Text PDF PubMed Google Scholar), or the developmental expression of another ADP-ribosyltransferase. The weak hybridization of the Yac-2 transferase cDNA with poly(A)+ RNA from cardiac and skeletal muscle may reflect the 59% nucleic acid sequence identity of the Yac-2 and skeletal muscle transferases. The hybridization of a Yac-2-specific oligonucleotide with poly(A)+ RNA from skeletal muscle, however, is consistent with the fact that both transferases are expressed in muscle. Based on three-dimensional structure, photoaffinity labeling, and site-directed mutagenesis, the bacterial toxin ADP-ribosyltransferases contain regions of similarity, which form, in part, an active-site pocket involved in NAD binding and nucleophilic attack on the N-glycosidic bond (23Rappuoli R. Pizza M. Alouf J.E. Freer J.H. Sourcebook of Bacterial Protein Toxins. Academic Press Inc., San Diego, CA1991: 1Google Scholar, 24Domenighini M. Magagnoli C. Pizza M. Rappuoli R. Mol. Microbiol. 1994; 14: 41-50Crossref PubMed Scopus (97) Google Scholar). The R-H region contains a nucleophilic arginine or histidine, and the acidic amino acid region contains the active-site glutamate (23Rappuoli R. Pizza M. Alouf J.E. Freer J.H. Sourcebook of Bacterial Protein Toxins. Academic Press Inc., San Diego, CA1991: 1Google Scholar). Alignment of the deduced amino acid sequences of the rabbit skeletal muscle transferase with those of the rodent RT6 proteins and several bacterial toxins and results from site-directed mutagenesis of the muscle enzyme (20Koch-Nolte F. Petersen D. Balasubramanian S. Haag F. Kahlke D. Willer T. Kastelein R. Bazan F. Thiele H.-G. J. Biol. Chem. 1996; 271: 7686-7693Abstract Full Text Full Text PDF PubMed Scopus (98) Google Scholar, 21Takada T. Iida K. Moss J. J. Biol. Chem. 1995; 270: 541-544Abstract Full Text Full Text PDF PubMed Scopus (59) Google Scholar) are consistent with the conclusion that the mammalian transferases possess consensus regions similar to those of the bacterial toxin transferases in the formation of the catalytic site. Likewise, alignment of the Yac-1, Yac-2, and rabbit muscle transferases suggests conservation of the postulated R-H region (Arg-174 of Yac-1 and Arg-161 of Yac-2) and active-site glutamates (Glu-233 and Glu-235 of Yac-1 and Glu-220 and Glu-222 of Yac-2) among the mammalian enzymes. Although both Glu-233 and Glu-235 are postulated to be critical for activity, Glu-235, based on alignment with the bacterial toxins, appears to be involved in ADP-ribosylation and corresponds to the glutamate in the bacterial toxins that was photocross-linked to nicotinamide (23Rappuoli R. Pizza M. Alouf J.E. Freer J.H. Sourcebook of Bacterial Protein Toxins. Academic Press Inc., San Diego, CA1991: 1Google Scholar, 25Carroll S.F. McCloskey J.A. Crain P.F. Oppenheimer N.J. Marschner T.M. Collier R.J. Proc. Natl. Acad. Sci. U. S. A. 1985; 82: 7237-7241Crossref PubMed Scopus (56) Google Scholar, 26Carroll S.F. Collier R.J. J. Biol. Chem. 1987; 262: 8707-8711Abstract Full Text PDF PubMed Google Scholar, 27Barbieri J.T. Mende-Mueller L.M. Rappuoli R. Collier R.J. Infect. Immun. 1989; 57: 3549-3554Crossref PubMed Google Scholar). The Yac-2 sequence contains an arginine at position 221, adjacent to the active-site glutamate, whereas the rabbit muscle and Yac-1 enzymes contain a glutamate at the corresponding position. Mutagenesis of this residue in the rabbit transferase, however, indicated that it was not crucial for activity (21Takada T. Iida K. Moss J. J. Biol. Chem. 1995; 270: 541-544Abstract Full Text Full Text PDF PubMed Scopus (59) Google Scholar). The rat T cell alloantigens RT6.1 and RT6.2 and the mouse Rt6-1 homologue possess NAD glycohydrolase activity (16Takada T. Iida K. Moss J. J. Biol. Chem. 1994; 269: 9420-9423Abstract Full Text PDF PubMed Google Scholar, 17Haag F. Andresen V. Karsten S. Koch-Nolte F. Thiele H.-G. Eur. J. Immunol. 1995; 25: 2355-2361Crossref PubMed Scopus (68) Google Scholar, 18Rigby M. Bortell R. Stevens L.A. Moss J. Kanaitsuka T. Shigeta H. Mordes J.P. Greiner D.L. Rossini A.A. J. Immunol. 1996; 156: 4259-4265PubMed Google Scholar, 19Maehama T. Nishina H. Hoshino S. Kanaho Y. Katada T. J. Biol. Chem. 1995; 270: 22747-22751Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar, 20Koch-Nolte F. Petersen D. Balasubramanian S. Haag F. Kahlke D. Willer T. Kastelein R. Bazan F. Thiele H.-G. J. Biol. Chem. 1996; 271: 7686-7693Abstract Full Text Full Text PDF PubMed Scopus (98) Google Scholar, 21Takada T. Iida K. Moss J. J. Biol. Chem. 1995; 270: 541-544Abstract Full Text Full Text PDF PubMed Scopus (59) Google Scholar). The RT6 proteins demonstrated auto-ADP-ribosyltransferase activity, and the recombinant mouse Rt6-1 homologue (but not the rat RT6.2 enzyme), expressed in the baculovirus system, was also capable of ADP-ribosylating histones (17Haag F. Andresen V. Karsten S. Koch-Nolte F. Thiele H.-G. Eur. J. Immunol. 1995; 25: 2355-2361Crossref PubMed Scopus (68) Google Scholar, 18Rigby M. Bortell R. Stevens L.A. Moss J. Kanaitsuka T. Shigeta H. Mordes J.P. Greiner D.L. Rossini A.A. J. Immunol. 1996; 156: 4259-4265PubMed Google Scholar, 19Maehama T. Nishina H. Hoshino S. Kanaho Y. Katada T. J. Biol. Chem. 1995; 270: 22747-22751Abstract Full Text Full Text PDF PubMed Scopus (38) Google Scholar). The absence of RT6+ T cells has been associated with the occurrence of autoimmune-mediated diabetes mellitus in diabetes-prone BioBreeding/Worchester rats (28Greiner D.L. Handler E.S. Nakano K. Mordes J.P. Rossini A.A. J. Immunol. 1986; 136: 148-151PubMed Google Scholar, 29Burstein D. Mordes J.P. Greiner D.L. Stein D. Nakamura N. Handler E.S. Rossini A.A. Diabetes. 1989; 38: 24-30Crossref PubMed Scopus (85) Google Scholar, 30Doukas J. Mordes J.P. J. Immunol. 1993; 150: 1036-1046PubMed Google Scholar). Diabetes can be prevented by the transfusion and long-term engraftment of RT6+ spleen cells (31Rossini A.A. Mordes J.P. Greiner D.L. Nakano K. Appel M.C. Handler E.S. J. Clin. Invest. 1986; 77: 1399-1401Crossref PubMed Scopus (39) Google Scholar). The non-obese diabetic mouse also has lower than normal levels of Rt6-specific mRNA and is prone to development of autoimmune-mediated diabetes (32Prochazka M. Leiter E.H. Serreze D.V. Coleman D.L. Science. 1987; 237: 286-289Crossref PubMed Scopus (253) Google Scholar). Alignment of the deduced amino acid sequences of the Yac-1 and Yac-2 transferases with those of rat RT6.1 and RT6.2 and mouse Rt6-1 demonstrates that the Yac-1 and Yac-2 enzymes are distinct from the RT6 family of proteins (Fig. 2). CD38, a differentiation antigen on the surface of lymphocytes that uses NAD as substrate, catalyzes the formation and hydrolysis of cyclic ADP-ribose, leading to the overall conversion of NAD to ADP-ribose and nicotinamide (33Howard M.C. Grimaldi J.C. Bazan J.F. Lund F.E. Santos-Argumedo L. Parkhouse R.M.E. Walseth T.F. Lee H.C. Science. 1993; 262: 1056-1059Crossref PubMed Scopus (666) Google Scholar). Cyclic ADP-ribose is a relatively recently recognized second messenger that can mobilize intracellular Ca2+ stores by an inositol 1,4,5-trisphosphate-independent mechanism (34Lee H.C. Walseth T.F. Bratt G.T. Hayes R.N. Clapper D.L. J. Biol. Chem. 1989; 264: 1608-1615Abstract Full Text PDF PubMed Google Scholar). Additionally, soluble CD38, expressed in a baculovirus system, ADP-ribosylated lysozyme, interleukin-2, and myoglobin by a nonenzymatic mechanism as a result of CD38-generated ADP-ribose becoming attached to cysteine residues via a thioglycosidic bond (35Grimaldi J.C. Balasubramanian S. Kabra N.H. Shanafelt A. Bazan J.F. Zurawski G. Howard M.C. J. Immunol. 1995; 155: 811-817PubMed Google Scholar). Although the Yac-2 protein, like CD38, has NAD glycohydrolase activity, it is also an arginine-specific ADP-ribosyltransferase, which synthesizes ADP-ribosylarginine by an Sn2-like mechanism that does not involve free ADP-ribose. The inhibition of proliferation of mouse CTLs by ADP-ribosylation of membrane proteins is consistent with the hypothesis that ADP-ribosyltransferases are involved in immune regulation (14Wang J. Nemoto E. Kots A.Y. Kaslow H.R. Dennert G. J. Immunol. 1994; 153: 4048-4058PubMed Google Scholar); the Yac-1 and Yac-2 transferases, along with other transferases/NAD glycohydrolases on lymphocytes such as CD38, may have a role in these processes. We thank Dr. Martha Vaughan for helpful discussions and critical review of this manuscript and Carol Kosh for expert secretarial assistance." @default.
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• W1965381503 title "Cloning and Characterization of a Novel Membrane-associated Lymphocyte NAD:Arginine ADP-ribosyltransferase" @default.
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