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- W1965402959 abstract "Abstract The recombinant neuraminidases (rNAs) from A/New Caledonia/20/99 (NC) (H1N1), A/PR/8/34 (PR8) (H1N1), A/Panama/2007/99 (Panama) (H3N2) and B/Shangdong/7/97 (B/S) viruses were produced by the CHO-K1 cells, prepared from clones which were selected based on the high expression of NA on the cell surface. Each rNA in the cell lysate was purified by an immuno-affinity column. The purified rNAs were obtained at the levels of approximately 0.6–1.8 mg/l medium. Properties of the recombinant N1-NA from NC virus was compared with other rNAs from PR8, Panama and B/S viruses, because N1-NA remained to be characterized. The results demonstrated that the immunogenicity of N1-rNA molecules, such as NC-NA and PR8-NA, was conserved partially even under the absence of the NA enzymatic activity, although the N1-rNA molecules were more labile than Panama-N2-rNA and B/S-rNA. They also suggested that N1-rNAs could be used as a useful protective antigen, capable of producing cross-protection among N1 influenza viruses when administered intranasally." @default.
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- W1965402959 date "2004-06-01" @default.
- W1965402959 modified "2023-09-27" @default.
- W1965402959 title "Expression of influenza neuraminidase in CHO-K1 cells" @default.
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- W1965402959 doi "https://doi.org/10.1016/j.ics.2004.02.117" @default.
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