FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965479323> ?p ?o ?g. }

• W1965479323 endingPage "383" @default.
• W1965479323 startingPage "375" @default.
• W1965479323 abstract "A processing endoribonuclease was isolated from the cytoplasm of chick embryos. The enzyme was easily obtained using an RNA extraction procedure based on a mild deproteinization with Sarkosyl and cold phenol/chloroform. This technique assured the recovery of several proteins and the endoribonuclease in association with the RNA. It was demonstrated that this endoribonuclease was capable of promoting, in vitro, a precise processing of naked 45-S ribosomal RNA precursor to molecules resembling the intermediates as well as the 28-S and 18-S cytoplasmic RNAs found in vivo. The presence of magnesium ions was required for the correct processing function of the enzyme. In addition, under the same conditions, the mature ribosomal RNA substrates were degraded at a slower rate by this RNA-associated RNase. It was possible to fractionate the enzymatic preparation into two different populations by means of a sucrose gradient: one associated and the other partially free of an RNA component. The effect of the intrinsic RNA associated with the endoribonuclease on the enzymatic activity was tested by analyzing both the enzymatic populations and the total enzymatic preparation treated with pronase or with immobilized pancreatic RNase. In all cases in which the RNA component was present, the enzyme showed processing activity. On the other hand, when the RNA component was absent or at least partially degraded the enzyme proved to be more active in processing precursor molecules and in promoting extensive degradation of mature RNA species. Although the presence of RNA in association with the enzyme was demonstrated, its role in the regulation of the enzymatic activity is yet not clear." @default.
• W1965479323 created "2016-06-24" @default.
• W1965479323 creator A5017054224 @default.
• W1965479323 creator A5047250037 @default.
• W1965479323 creator A5048964966 @default.
• W1965479323 creator A5068093648 @default.
• W1965479323 creator A5087808358 @default.
• W1965479323 date "1981-04-01" @default.
• W1965479323 modified "2023-09-27" @default.
• W1965479323 title "Processing of Naked 45-S Ribosomal RNA Precursor in vitro by an RNA-Associated Endoribonuclease" @default.
• W1965479323 cites W1482645770 @default.
• W1965479323 cites W1483456888 @default.
• W1965479323 cites W1490163585 @default.
• W1965479323 cites W1506337972 @default.
• W1965479323 cites W1527606153 @default.
• W1965479323 cites W1528919862 @default.
• W1965479323 cites W1539225833 @default.
• W1965479323 cites W1543686981 @default.
• W1965479323 cites W1685437327 @default.
• W1965479323 cites W1775749144 @default.
• W1965479323 cites W1834198679 @default.
• W1965479323 cites W1966231215 @default.
• W1965479323 cites W1975133018 @default.
• W1965479323 cites W1987869179 @default.
• W1965479323 cites W1989915900 @default.
• W1965479323 cites W1990791306 @default.
• W1965479323 cites W1993239341 @default.
• W1965479323 cites W1999608841 @default.
• W1965479323 cites W2004406095 @default.
• W1965479323 cites W2011358721 @default.
• W1965479323 cites W2013031557 @default.
• W1965479323 cites W2014286993 @default.
• W1965479323 cites W2016309696 @default.
• W1965479323 cites W2021017070 @default.
• W1965479323 cites W2024553286 @default.
• W1965479323 cites W2026487244 @default.
• W1965479323 cites W2035751785 @default.
• W1965479323 cites W2038707341 @default.
• W1965479323 cites W2051060622 @default.
• W1965479323 cites W2051066652 @default.
• W1965479323 cites W2066676576 @default.
• W1965479323 cites W2072257886 @default.
• W1965479323 cites W2073286556 @default.
• W1965479323 cites W2074232368 @default.
• W1965479323 cites W2078691498 @default.
• W1965479323 cites W2079936702 @default.
• W1965479323 cites W2084006277 @default.
• W1965479323 cites W2084381734 @default.
• W1965479323 cites W2086629852 @default.
• W1965479323 cites W2090496129 @default.
• W1965479323 cites W2100837269 @default.
• W1965479323 cites W2111331109 @default.
• W1965479323 cites W2128635872 @default.
• W1965479323 cites W39064870 @default.
• W1965479323 doi "https://doi.org/10.1111/j.1432-1033.1981.tb05248.x" @default.
• W1965479323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6165585" @default.
• W1965479323 hasPublicationYear "1981" @default.
• W1965479323 type Work @default.
• W1965479323 sameAs 1965479323 @default.
• W1965479323 citedByCount "7" @default.
• W1965479323 crossrefType "journal-article" @default.
• W1965479323 hasAuthorship W1965479323A5017054224 @default.
• W1965479323 hasAuthorship W1965479323A5047250037 @default.
• W1965479323 hasAuthorship W1965479323A5048964966 @default.
• W1965479323 hasAuthorship W1965479323A5068093648 @default.
• W1965479323 hasAuthorship W1965479323A5087808358 @default.
• W1965479323 hasBestOaLocation W19654793231 @default.
• W1965479323 hasConcept C104317684 @default.
• W1965479323 hasConcept C105580179 @default.
• W1965479323 hasConcept C108190873 @default.
• W1965479323 hasConcept C10879258 @default.
• W1965479323 hasConcept C153911025 @default.
• W1965479323 hasConcept C181199279 @default.
• W1965479323 hasConcept C185592680 @default.
• W1965479323 hasConcept C2780102198 @default.
• W1965479323 hasConcept C41258723 @default.
• W1965479323 hasConcept C55493867 @default.
• W1965479323 hasConcept C67705224 @default.
• W1965479323 hasConcept C67905577 @default.
• W1965479323 hasConcept C75449159 @default.
• W1965479323 hasConcept C86803240 @default.
• W1965479323 hasConceptScore W1965479323C104317684 @default.
• W1965479323 hasConceptScore W1965479323C105580179 @default.
• W1965479323 hasConceptScore W1965479323C108190873 @default.
• W1965479323 hasConceptScore W1965479323C10879258 @default.
• W1965479323 hasConceptScore W1965479323C153911025 @default.
• W1965479323 hasConceptScore W1965479323C181199279 @default.
• W1965479323 hasConceptScore W1965479323C185592680 @default.
• W1965479323 hasConceptScore W1965479323C2780102198 @default.
• W1965479323 hasConceptScore W1965479323C41258723 @default.
• W1965479323 hasConceptScore W1965479323C55493867 @default.
• W1965479323 hasConceptScore W1965479323C67705224 @default.
• W1965479323 hasConceptScore W1965479323C67905577 @default.
• W1965479323 hasConceptScore W1965479323C75449159 @default.
• W1965479323 hasConceptScore W1965479323C86803240 @default.
• W1965479323 hasIssue "2" @default.
• W1965479323 hasLocation W19654793231 @default.
• W1965479323 hasLocation W19654793232 @default.

• next