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• W1965487513 abstract "We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29-60 nm in the focal plane, corresponding to a 5-8-fold improvement over the diffraction barrier. Axial spot confinement increased the axial resolution by 3.5-fold. We observed three-dimensional (3D) subdiffraction resolution in 3D image stacks. Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy." @default.
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• W1965487513 date "2007-10-21" @default.
• W1965487513 modified "2023-10-14" @default.
• W1965487513 title "STED microscopy with continuous wave beams" @default.
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• W1965487513 doi "https://doi.org/10.1038/nmeth1108" @default.
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