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- W1965505038 abstract "Nuclei from TSH-treated and control thyroid slices were subjected to very limited digestion by DNase I, and then centrifuged at 1200×g. The amount of DNA released into supernatants was increased significantly by TSH when <0.1% to 3% of total DNA was rendered acid-soluble. This effect could be detected in buffers containing 2mM Mg++ (with chromatin condensed) or <0.05mM Mg++ (chromatin decondensed). Gel electrophoresis showed that the length of the majority of the DNA fragments in the supernatants (<0.1% of DNA acid-soluble) was greater than 4 kilobases; no TSH-dependent shift in size distribution was observed. We speculate that TSH may affect specific DNase I-hypersensitive sites in chromatin." @default.
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- W1965505038 date "1982-04-01" @default.
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- W1965505038 title "TSH-treatment of thyroid slices increases the amount of DNA released from nuclei by mild DNase-I digestion" @default.
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- W1965505038 doi "https://doi.org/10.1016/0006-291x(82)91083-x" @default.
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