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• W1965536658 abstract "The uptake of polycyclic aromatic hydrocarbons (PAHs) by fish can be determined by screening the gall bladder bile for PAH metabolites. Conjugated 1-hydroxy pyrene is a major metabolite in bile of fish exposed to PAH polluted sediment. Synchronous fluorescence spectrometry is presented as a rapid screening technique for the determination of this compound; the complete analysis takes only 2–3 min. HPLC with fluorescence detection was used to validate the assay. Calibration methods, using either free 1-hydroxy pyrene or the pyrene-1-glucuronide conjugate as a reference standard, were investigated. The technique was applied to a mesocosm study in which the uptake of PAHs by flounder (Platichthys flesus) from polluted sediment was studied. Direct contact with the sediment proved the most important factor; the uptake through water phase or diet was less significant. The usefulness of 1-hydroxy pyrene analysis in bile as a monitoring tool for PAH exposure is discussed." @default.
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• W1965536658 date "1993-08-01" @default.
• W1965536658 modified "2023-10-14" @default.
• W1965536658 title "Synchronous fluorescence spectrometry of fish bile: A rapid screening method for the biomonitoring of PAH exposure" @default.
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• W1965536658 doi "https://doi.org/10.1016/0166-445x(93)90034-x" @default.
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