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- W1965556237 abstract "30S ribosomes were isolated from a kasugamycin resistant mutant of E. coli that lacks methylgroups on two adjacent adenines in 16S ribsomal RNA. These ribosomes were methylated in vitro with a purified methylating enzyme and 5-S-adenosyl- ( 13 C-mathl) -L-methionine chloride (( 13 C-methyl) -SAM) as methyldonor. After in situ cleavage of the 16S ribosomal RNA by the bacteriocin cloacin DF 13 , the 49 nucleotide fragment from the 3'-end of the RNA was isolated. The carbon-13 nuclear magnetic resonance spectra of the fragment at various tanperatures were compared with those of 6-N-dimethyladenosine (m 26 A) and 6-N-dimethyladenylyl- (3'→5') -6-N-dimethyladenosine (m 26 Am 26 A). The data show that the two methylated adenines, which are part of a four membered hairpin loop show a strong tendency to be stacked in analogy to the dinucleotide m 26 Am 26 A." @default.
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- W1965556237 date "1982-01-01" @default.
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- W1965556237 title "A carbon-13 neclear magnetic resonance study of the 3'-terminus of 16S ribosomal RNA of<i>Escherichia coli</i>specifically labeled with carbon-13 in the methlgroups of the m<sub>2</sub><sup>6</sup>Am<sub>2</sub><sup>6</sup>A sequence" @default.
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- W1965556237 doi "https://doi.org/10.1093/nar/10.14.4237" @default.
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