Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965571730> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1965571730 endingPage "351" @default.
- W1965571730 startingPage "343" @default.
- W1965571730 abstract "Various incubation conditions previously used in cell-free assays of steroid receptor binding to nuclei were evaluated on the basis of their effect on the integrity of the nuclear chromatin. Incubation of nuclei for 60 min in whole cytosol containing no added salt at 25°C resulted in significant DNA degradation and some proteolysis. This deterioration in the integrity of the chromatin increased its capacity to bind the [3H]-progesterone-receptor complex, possibly via exposure of previously “masked” acceptor sites. Decreasing the temperature and/or increasing the salt concentration reduced or eliminated these changes in the chromatin. Incubation of nuclei in an ammonium sulfate-fraction of the cytosol, which contained the partially purified progesterone receptor, resulted in no detectable deterioration of the chromatin integrity irrespective of the temperature (⩽25°C) or the added salt concentration (0–0.15 M KCl). These results suggest that some of the conflicting reports on the nature of the nuclear acceptor sites for steroid receptors could be due to the use of inappropriate assay conditions, which permit degradation of the nucleo-protein and result in altered levels of steroid receptor binding. (Steroid/ Progesterone/Nuclei/Chromatin.)" @default.
- W1965571730 created "2016-06-24" @default.
- W1965571730 creator A5054911795 @default.
- W1965571730 creator A5066880464 @default.
- W1965571730 date "1979-04-01" @default.
- W1965571730 modified "2023-10-16" @default.
- W1965571730 title "Steroid receptor binding to nuclei: Effect of assay conditions on the integrity of chromatin" @default.
- W1965571730 cites W1503919996 @default.
- W1965571730 cites W1535177777 @default.
- W1965571730 cites W1549614989 @default.
- W1965571730 cites W1563004320 @default.
- W1965571730 cites W1573627148 @default.
- W1965571730 cites W1596341727 @default.
- W1965571730 cites W1775749144 @default.
- W1965571730 cites W1906197854 @default.
- W1965571730 cites W1963721376 @default.
- W1965571730 cites W1985412164 @default.
- W1965571730 cites W1992258926 @default.
- W1965571730 cites W2019725919 @default.
- W1965571730 cites W2021017764 @default.
- W1965571730 cites W2021104306 @default.
- W1965571730 cites W2028895755 @default.
- W1965571730 cites W2078583028 @default.
- W1965571730 cites W2079028818 @default.
- W1965571730 cites W2082413987 @default.
- W1965571730 cites W2087803718 @default.
- W1965571730 cites W2093144471 @default.
- W1965571730 cites W2094082118 @default.
- W1965571730 cites W2225182560 @default.
- W1965571730 cites W2232440791 @default.
- W1965571730 cites W2240726600 @default.
- W1965571730 cites W2280486030 @default.
- W1965571730 cites W2280890417 @default.
- W1965571730 doi "https://doi.org/10.1016/0022-4731(79)90318-2" @default.
- W1965571730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/449309" @default.
- W1965571730 hasPublicationYear "1979" @default.
- W1965571730 type Work @default.
- W1965571730 sameAs 1965571730 @default.
- W1965571730 citedByCount "14" @default.
- W1965571730 crossrefType "journal-article" @default.
- W1965571730 hasAuthorship W1965571730A5054911795 @default.
- W1965571730 hasAuthorship W1965571730A5066880464 @default.
- W1965571730 hasConcept C12554922 @default.
- W1965571730 hasConcept C153911025 @default.
- W1965571730 hasConcept C170493617 @default.
- W1965571730 hasConcept C181199279 @default.
- W1965571730 hasConcept C185592680 @default.
- W1965571730 hasConcept C190062978 @default.
- W1965571730 hasConcept C25642318 @default.
- W1965571730 hasConcept C2780114586 @default.
- W1965571730 hasConcept C2780902042 @default.
- W1965571730 hasConcept C2781307694 @default.
- W1965571730 hasConcept C552990157 @default.
- W1965571730 hasConcept C55493867 @default.
- W1965571730 hasConcept C71315377 @default.
- W1965571730 hasConcept C83640560 @default.
- W1965571730 hasConcept C86803240 @default.
- W1965571730 hasConcept C98539663 @default.
- W1965571730 hasConceptScore W1965571730C12554922 @default.
- W1965571730 hasConceptScore W1965571730C153911025 @default.
- W1965571730 hasConceptScore W1965571730C170493617 @default.
- W1965571730 hasConceptScore W1965571730C181199279 @default.
- W1965571730 hasConceptScore W1965571730C185592680 @default.
- W1965571730 hasConceptScore W1965571730C190062978 @default.
- W1965571730 hasConceptScore W1965571730C25642318 @default.
- W1965571730 hasConceptScore W1965571730C2780114586 @default.
- W1965571730 hasConceptScore W1965571730C2780902042 @default.
- W1965571730 hasConceptScore W1965571730C2781307694 @default.
- W1965571730 hasConceptScore W1965571730C552990157 @default.
- W1965571730 hasConceptScore W1965571730C55493867 @default.
- W1965571730 hasConceptScore W1965571730C71315377 @default.
- W1965571730 hasConceptScore W1965571730C83640560 @default.
- W1965571730 hasConceptScore W1965571730C86803240 @default.
- W1965571730 hasConceptScore W1965571730C98539663 @default.
- W1965571730 hasIssue "4" @default.
- W1965571730 hasLocation W19655717301 @default.
- W1965571730 hasLocation W19655717302 @default.
- W1965571730 hasOpenAccess W1965571730 @default.
- W1965571730 hasPrimaryLocation W19655717301 @default.
- W1965571730 hasRelatedWork W1986393148 @default.
- W1965571730 hasRelatedWork W2004754196 @default.
- W1965571730 hasRelatedWork W2015146829 @default.
- W1965571730 hasRelatedWork W2055571645 @default.
- W1965571730 hasRelatedWork W2083816668 @default.
- W1965571730 hasRelatedWork W2140350520 @default.
- W1965571730 hasRelatedWork W2413719344 @default.
- W1965571730 hasRelatedWork W2413950150 @default.
- W1965571730 hasRelatedWork W2416583487 @default.
- W1965571730 hasRelatedWork W2464620537 @default.
- W1965571730 hasVolume "10" @default.
- W1965571730 isParatext "false" @default.
- W1965571730 isRetracted "false" @default.
- W1965571730 magId "1965571730" @default.
- W1965571730 workType "article" @default.