FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965573411> ?p ?o ?g. }

• W1965573411 endingPage "311" @default.
• W1965573411 startingPage "301" @default.
• W1965573411 abstract "The effects of oxygen transfer on the production and product distribution in serine alkaline protease (SAP) fermentation by Bacillus licheniformis and oxygen-transfer strategy in relation to the physiology of the bacilli were investigated on a defined medium with citric acid as sole carbon source in 3.5-dm3 batch bioreactor systems. By forming a 3 × 3 matrix with the parameters air-inlet rates of QO/VR = 0.2, 0.5, 1.0 vvm, and agitation rates of N = 150, 500, 750 min−1, the effects of oxygen transfer were investigated at nine different conditions. The concentrations of the product SAP and by-products, i.e., neutral protease, α-amylase, amino acids, and organic acids, and SAP activities were determined throughout the bioprocess. Among the constant air-flow and agitation-rate fermentations, QO/VR = 0.5 vvm, N = 750 min−1 oxygen-transfer conditions produced maximum SAP activity that was 500 U cm−3, at t = 37 h. With the increase in QO/VR and/or N, Damköhler number that is the oxygen-transfer limitation decreases; and the process passes from oxygen-transfer limited conditions to biochemical-reaction limited conditions. Further increase in SAP activity, A = 680 U cm−3 was achieved by applying an oxygen-transfer strategy based on the analysis of the data obtained with the constant oxygen-transfer condition experiments, with a step increase in air-inlet rate, from QO/VR = 0.2 to QO/VR = 0.5 vvm at N = 750 min−1 constant agitation rate at t = 24 h. Organic acids and amino acids that were excreted to the fermentation medium varied depending on the oxygen-transfer conditions. With the increase in oxygen-transfer rate acetic acid concentration increased; contrarily, with the decrease in the oxygen-transfer rate the TCA-cycle organic acids α-ketoglutaric and succinic acids, and gluconic acid were excreted to the fermentation broth; nevertheless, the application of the oxygen-transfer strategy prevented the increase in acetic acid concentration between t = 35–38 h. Under all the oxygen-transfer conditions, the amino acid having the highest concentration and the amino acid that was not excreted to the fermentation broth were lysine and asparagine, respectively; both of which belong to the aspartic acid-group amino acids. Further, this result indicates the requirement of the genetic regulation directed to the aspartic acid-group enzymes for the progress in SAP production in B. licheniformis. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 69: 301–311, 2000." @default.
• W1965573411 created "2016-06-24" @default.
• W1965573411 creator A5027255182 @default.
• W1965573411 creator A5078213491 @default.
• W1965573411 creator A5088253996 @default.
• W1965573411 date "2000-01-01" @default.
• W1965573411 modified "2023-10-11" @default.
• W1965573411 title "Oxygen-transfer strategy and its regulation effects in serine alkaline protease production byBacillus licheniformis" @default.
• W1965573411 cites W1535272956 @default.
• W1965573411 cites W1967647423 @default.
• W1965573411 cites W1978682976 @default.
• W1965573411 cites W1992926978 @default.
• W1965573411 cites W1999439881 @default.
• W1965573411 cites W2053460679 @default.
• W1965573411 cites W2053666022 @default.
• W1965573411 cites W2079022179 @default.
• W1965573411 cites W2093634492 @default.
• W1965573411 cites W2127851374 @default.
• W1965573411 cites W2155025679 @default.
• W1965573411 cites W2160824669 @default.
• W1965573411 cites W4211180308 @default.
• W1965573411 cites W4239933124 @default.
• W1965573411 cites W596970151 @default.
• W1965573411 doi "https://doi.org/10.1002/1097-0290(20000805)69:3<301::aid-bit8>3.0.co;2-4" @default.
• W1965573411 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10861410" @default.
• W1965573411 hasPublicationYear "2000" @default.
• W1965573411 type Work @default.
• W1965573411 sameAs 1965573411 @default.
• W1965573411 citedByCount "76" @default.
• W1965573411 countsByYear W19655734112012 @default.
• W1965573411 countsByYear W19655734112013 @default.
• W1965573411 countsByYear W19655734112014 @default.
• W1965573411 countsByYear W19655734112015 @default.
• W1965573411 countsByYear W19655734112016 @default.
• W1965573411 countsByYear W19655734112017 @default.
• W1965573411 countsByYear W19655734112018 @default.
• W1965573411 countsByYear W19655734112019 @default.
• W1965573411 countsByYear W19655734112022 @default.
• W1965573411 crossrefType "journal-article" @default.
• W1965573411 hasAuthorship W1965573411A5027255182 @default.
• W1965573411 hasAuthorship W1965573411A5078213491 @default.
• W1965573411 hasAuthorship W1965573411A5088253996 @default.
• W1965573411 hasConcept C100544194 @default.
• W1965573411 hasConcept C168170006 @default.
• W1965573411 hasConcept C178790620 @default.
• W1965573411 hasConcept C185592680 @default.
• W1965573411 hasConcept C2776527165 @default.
• W1965573411 hasConcept C2777272437 @default.
• W1965573411 hasConcept C43617362 @default.
• W1965573411 hasConcept C523546767 @default.
• W1965573411 hasConcept C540031477 @default.
• W1965573411 hasConcept C54355233 @default.
• W1965573411 hasConcept C55493867 @default.
• W1965573411 hasConcept C73401843 @default.
• W1965573411 hasConcept C86803240 @default.
• W1965573411 hasConceptScore W1965573411C100544194 @default.
• W1965573411 hasConceptScore W1965573411C168170006 @default.
• W1965573411 hasConceptScore W1965573411C178790620 @default.
• W1965573411 hasConceptScore W1965573411C185592680 @default.
• W1965573411 hasConceptScore W1965573411C2776527165 @default.
• W1965573411 hasConceptScore W1965573411C2777272437 @default.
• W1965573411 hasConceptScore W1965573411C43617362 @default.
• W1965573411 hasConceptScore W1965573411C523546767 @default.
• W1965573411 hasConceptScore W1965573411C540031477 @default.
• W1965573411 hasConceptScore W1965573411C54355233 @default.
• W1965573411 hasConceptScore W1965573411C55493867 @default.
• W1965573411 hasConceptScore W1965573411C73401843 @default.
• W1965573411 hasConceptScore W1965573411C86803240 @default.
• W1965573411 hasIssue "3" @default.
• W1965573411 hasLocation W19655734111 @default.
• W1965573411 hasLocation W19655734112 @default.
• W1965573411 hasOpenAccess W1965573411 @default.
• W1965573411 hasPrimaryLocation W19655734111 @default.
• W1965573411 hasRelatedWork W1988476430 @default.
• W1965573411 hasRelatedWork W2351545764 @default.
• W1965573411 hasRelatedWork W2356401515 @default.
• W1965573411 hasRelatedWork W2361104205 @default.
• W1965573411 hasRelatedWork W2383463807 @default.
• W1965573411 hasRelatedWork W2389022589 @default.
• W1965573411 hasRelatedWork W2394030186 @default.
• W1965573411 hasRelatedWork W2566341560 @default.
• W1965573411 hasRelatedWork W2579924761 @default.
• W1965573411 hasRelatedWork W2992409559 @default.
• W1965573411 hasVolume "69" @default.
• W1965573411 isParatext "false" @default.
• W1965573411 isRetracted "false" @default.
• W1965573411 magId "1965573411" @default.
• W1965573411 workType "article" @default.