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- W1965585643 abstract "Abstract Eight strains of sake yeast exhibited inherent-resistance to 100 μg/ml of Geneticin (G418). Fourteen wine yeasts and 1 shochu yeast ( Saccharomyces cerevisiae ) and 1 miso yeast ( Zygosaccharomyces rouxii ) were inherent G418-sensitive. The petites converted from inherent G418-resistants by treatment with ethidium bromide retained G418-resistance (ϱ − G418R), and thus were hybridized by electrofusion with the wine yeast W3 (ϱ + G418S, wild type). A lag phase of 12–18 h was required prior to administration of the drug in glycerol medium when selecting G418-resistant hybridization products. Colonies were formed in the regeneration medium at a frequency of about 1 × 10 −5 per used protoplasts. No growth of any parental strain (10 6 /_~10 7 protoplasts) separately subjected to electrofusion and regeneration was observed. The hybridization products were G418-resistant “grande” strains (ϱ − G418R) in which the genetic traits of parental strains had been complemented. Uninucleate cells (DAPI staining) of the hybridization products showed CHEF electrophoretic karyotypes similar to that of wine yeast, but possessed a single chromosome (approx. 320 kb) presumably from sake yeast." @default.
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- W1965585643 date "1994-01-01" @default.
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- W1965585643 title "Inherent G418-resistance in hybridization of industrial yeasts" @default.
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- W1965585643 doi "https://doi.org/10.1016/0922-338x(94)90325-5" @default.
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