Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965600676> ?p ?o ?g. }
- W1965600676 endingPage "294" @default.
- W1965600676 startingPage "285" @default.
- W1965600676 abstract "The triazine dyes, Cibacron blue F3GA and Procion red HE3B inhibited diaphorase activity of ferredoxin-NADP+ reductase, in a competitive manner with respect to NADPH. The Ki values were 1.5 and 0.2 microM, respectively. Binding of the dyes to the flavoprotein, as measured by difference spectroscopy, indicated an apparent stoichiometry of 1 mol dye/mol reductase and was prevented by NADP+ or high ionic strength. Chemical modification of a lysine residue and a carboxyl group at the NADP(H) binding site of the enzyme prevented complex formation with Procion red. Procion red showed a higher affinity for ferredoxin-NADP+ reductase than Cibacron blue. The Kd values were 1.9 and 5 microM, respectively. Once covalently linked to a Sepharose matrix, the triazine compounds specifically bind the flavoprotein. The interaction is partially electrostatic and partially hydrophobic. The enzyme can be eluted by high concentrations of salt or low concentrations of the corresponding coenzyme. The use of this affinity column allows the rapid purification of ferredoxin-NADP+ oxidoreductase from spinach leaves with good yields." @default.
- W1965600676 created "2016-06-24" @default.
- W1965600676 creator A5002305226 @default.
- W1965600676 creator A5087007557 @default.
- W1965600676 date "1983-01-01" @default.
- W1965600676 modified "2023-09-27" @default.
- W1965600676 title "Interaction of ferredoxin-NADP+ oxidoreductase with triazine dyes a rapid purification method by affinity chromatography" @default.
- W1965600676 cites W1422649125 @default.
- W1965600676 cites W1526571049 @default.
- W1965600676 cites W1598846697 @default.
- W1965600676 cites W1604233325 @default.
- W1965600676 cites W1767036196 @default.
- W1965600676 cites W1775749144 @default.
- W1965600676 cites W1988012975 @default.
- W1965600676 cites W1994051581 @default.
- W1965600676 cites W2000411386 @default.
- W1965600676 cites W2000489749 @default.
- W1965600676 cites W2007615331 @default.
- W1965600676 cites W2007829736 @default.
- W1965600676 cites W2007936866 @default.
- W1965600676 cites W2013142271 @default.
- W1965600676 cites W2013277117 @default.
- W1965600676 cites W2027125634 @default.
- W1965600676 cites W2035521743 @default.
- W1965600676 cites W2039093129 @default.
- W1965600676 cites W2044873655 @default.
- W1965600676 cites W2059962043 @default.
- W1965600676 cites W2068230596 @default.
- W1965600676 cites W2068646004 @default.
- W1965600676 cites W2074235288 @default.
- W1965600676 cites W2087500173 @default.
- W1965600676 cites W2088442562 @default.
- W1965600676 cites W2089136609 @default.
- W1965600676 cites W2089648476 @default.
- W1965600676 cites W2091457795 @default.
- W1965600676 cites W2093567873 @default.
- W1965600676 cites W2111301002 @default.
- W1965600676 cites W234127572 @default.
- W1965600676 cites W2344694530 @default.
- W1965600676 cites W2416843073 @default.
- W1965600676 cites W4248497761 @default.
- W1965600676 cites W75650367 @default.
- W1965600676 cites W93979582 @default.
- W1965600676 cites W957475716 @default.
- W1965600676 cites W966549641 @default.
- W1965600676 doi "https://doi.org/10.1016/0167-4838(83)90313-8" @default.
- W1965600676 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6824690" @default.
- W1965600676 hasPublicationYear "1983" @default.
- W1965600676 type Work @default.
- W1965600676 sameAs 1965600676 @default.
- W1965600676 citedByCount "23" @default.
- W1965600676 countsByYear W19656006762012 @default.
- W1965600676 crossrefType "journal-article" @default.
- W1965600676 hasAuthorship W1965600676A5002305226 @default.
- W1965600676 hasAuthorship W1965600676A5087007557 @default.
- W1965600676 hasConcept C134621786 @default.
- W1965600676 hasConcept C134651460 @default.
- W1965600676 hasConcept C178790620 @default.
- W1965600676 hasConcept C179247698 @default.
- W1965600676 hasConcept C181199279 @default.
- W1965600676 hasConcept C184651966 @default.
- W1965600676 hasConcept C185592680 @default.
- W1965600676 hasConcept C187428577 @default.
- W1965600676 hasConcept C197957613 @default.
- W1965600676 hasConcept C2776303721 @default.
- W1965600676 hasConcept C2776961318 @default.
- W1965600676 hasConcept C2779268744 @default.
- W1965600676 hasConcept C2779881004 @default.
- W1965600676 hasConcept C43617362 @default.
- W1965600676 hasConcept C55493867 @default.
- W1965600676 hasConcept C71240020 @default.
- W1965600676 hasConcept C71995715 @default.
- W1965600676 hasConceptScore W1965600676C134621786 @default.
- W1965600676 hasConceptScore W1965600676C134651460 @default.
- W1965600676 hasConceptScore W1965600676C178790620 @default.
- W1965600676 hasConceptScore W1965600676C179247698 @default.
- W1965600676 hasConceptScore W1965600676C181199279 @default.
- W1965600676 hasConceptScore W1965600676C184651966 @default.
- W1965600676 hasConceptScore W1965600676C185592680 @default.
- W1965600676 hasConceptScore W1965600676C187428577 @default.
- W1965600676 hasConceptScore W1965600676C197957613 @default.
- W1965600676 hasConceptScore W1965600676C2776303721 @default.
- W1965600676 hasConceptScore W1965600676C2776961318 @default.
- W1965600676 hasConceptScore W1965600676C2779268744 @default.
- W1965600676 hasConceptScore W1965600676C2779881004 @default.
- W1965600676 hasConceptScore W1965600676C43617362 @default.
- W1965600676 hasConceptScore W1965600676C55493867 @default.
- W1965600676 hasConceptScore W1965600676C71240020 @default.
- W1965600676 hasConceptScore W1965600676C71995715 @default.
- W1965600676 hasIssue "2" @default.
- W1965600676 hasLocation W19656006761 @default.
- W1965600676 hasLocation W19656006762 @default.
- W1965600676 hasOpenAccess W1965600676 @default.
- W1965600676 hasPrimaryLocation W19656006761 @default.
- W1965600676 hasRelatedWork W1547072346 @default.
- W1965600676 hasRelatedWork W1965600676 @default.
- W1965600676 hasRelatedWork W2006352258 @default.
- W1965600676 hasRelatedWork W2006369456 @default.