Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965647360> ?p ?o ?g. }
- W1965647360 endingPage "339" @default.
- W1965647360 startingPage "333" @default.
- W1965647360 abstract "The mutant 554 of TEM-2 β-lactamase was selected for a decrease in the resistance to carbenicillin of an Escherichia coli K12 carrier. The amino acid sequence of the mutant β-lactamase was determined by manual Edman degradation analysis of proteolytic peptides. A single substitution Val for Ala was localized at position 237. The mutant exhibited only 2% of the catalytic efficiency of the wild-type enzyme towards carbenicillin and ticarcillin, whereas it retained 30–60% of the hydrolytic activity towards other penicillin and cephalosporin substrates. Carfecillin, the phenyl ester of the side-chain carboxyl group of carbenicillin, was hydrolysed as a good substrate. This suggests that the behaviour of the mutant enzyme towards carbenicillin may result from ionic rather than steric constraints. A molecular model of the Val-237 TEM-2 mutant suggests possible electrostatic interaction between Glu-171 and the carboxylic group of the side chain of carbenicillin." @default.
- W1965647360 created "2016-06-24" @default.
- W1965647360 creator A5002921201 @default.
- W1965647360 creator A5012166685 @default.
- W1965647360 creator A5022157667 @default.
- W1965647360 creator A5078131531 @default.
- W1965647360 creator A5086550863 @default.
- W1965647360 creator A5086944938 @default.
- W1965647360 date "1994-04-01" @default.
- W1965647360 modified "2023-10-17" @default.
- W1965647360 title "Val-237 for Ala substitution in the TEM-2 β-lactamase dramatically alters the catalytic efficiencies towards carbenicillin and ticarcillin" @default.
- W1965647360 cites W1498996826 @default.
- W1965647360 cites W1501315577 @default.
- W1965647360 cites W1501616836 @default.
- W1965647360 cites W1520817078 @default.
- W1965647360 cites W1543749024 @default.
- W1965647360 cites W1787342943 @default.
- W1965647360 cites W1861263514 @default.
- W1965647360 cites W1908101661 @default.
- W1965647360 cites W1922438834 @default.
- W1965647360 cites W1954141158 @default.
- W1965647360 cites W1969222445 @default.
- W1965647360 cites W1969435865 @default.
- W1965647360 cites W1972310685 @default.
- W1965647360 cites W1976915850 @default.
- W1965647360 cites W1977451966 @default.
- W1965647360 cites W1980383154 @default.
- W1965647360 cites W1983477347 @default.
- W1965647360 cites W2004228048 @default.
- W1965647360 cites W2006948287 @default.
- W1965647360 cites W2016668411 @default.
- W1965647360 cites W2023755203 @default.
- W1965647360 cites W2041893228 @default.
- W1965647360 cites W2047709322 @default.
- W1965647360 cites W2051687036 @default.
- W1965647360 cites W2060016760 @default.
- W1965647360 cites W2073857103 @default.
- W1965647360 cites W2076801181 @default.
- W1965647360 cites W2078340635 @default.
- W1965647360 cites W2094306541 @default.
- W1965647360 cites W2094326217 @default.
- W1965647360 cites W2113072432 @default.
- W1965647360 cites W2119630062 @default.
- W1965647360 cites W2143874510 @default.
- W1965647360 cites W2144362435 @default.
- W1965647360 cites W2147872249 @default.
- W1965647360 cites W2153279608 @default.
- W1965647360 cites W2254663427 @default.
- W1965647360 cites W2256640516 @default.
- W1965647360 cites W2271272384 @default.
- W1965647360 doi "https://doi.org/10.1111/j.1574-6968.1994.tb06788.x" @default.
- W1965647360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8200509" @default.
- W1965647360 hasPublicationYear "1994" @default.
- W1965647360 type Work @default.
- W1965647360 sameAs 1965647360 @default.
- W1965647360 citedByCount "0" @default.
- W1965647360 crossrefType "journal-article" @default.
- W1965647360 hasAuthorship W1965647360A5002921201 @default.
- W1965647360 hasAuthorship W1965647360A5012166685 @default.
- W1965647360 hasAuthorship W1965647360A5022157667 @default.
- W1965647360 hasAuthorship W1965647360A5078131531 @default.
- W1965647360 hasAuthorship W1965647360A5086550863 @default.
- W1965647360 hasAuthorship W1965647360A5086944938 @default.
- W1965647360 hasBestOaLocation W19656473601 @default.
- W1965647360 hasConcept C104317684 @default.
- W1965647360 hasConcept C143065580 @default.
- W1965647360 hasConcept C178790620 @default.
- W1965647360 hasConcept C181199279 @default.
- W1965647360 hasConcept C185592680 @default.
- W1965647360 hasConcept C204921945 @default.
- W1965647360 hasConcept C2776021129 @default.
- W1965647360 hasConcept C2776083883 @default.
- W1965647360 hasConcept C2779631663 @default.
- W1965647360 hasConcept C2779913634 @default.
- W1965647360 hasConcept C501593827 @default.
- W1965647360 hasConcept C521977710 @default.
- W1965647360 hasConcept C547475151 @default.
- W1965647360 hasConcept C55493867 @default.
- W1965647360 hasConcept C71240020 @default.
- W1965647360 hasConcept C94412978 @default.
- W1965647360 hasConcept C94665300 @default.
- W1965647360 hasConceptScore W1965647360C104317684 @default.
- W1965647360 hasConceptScore W1965647360C143065580 @default.
- W1965647360 hasConceptScore W1965647360C178790620 @default.
- W1965647360 hasConceptScore W1965647360C181199279 @default.
- W1965647360 hasConceptScore W1965647360C185592680 @default.
- W1965647360 hasConceptScore W1965647360C204921945 @default.
- W1965647360 hasConceptScore W1965647360C2776021129 @default.
- W1965647360 hasConceptScore W1965647360C2776083883 @default.
- W1965647360 hasConceptScore W1965647360C2779631663 @default.
- W1965647360 hasConceptScore W1965647360C2779913634 @default.
- W1965647360 hasConceptScore W1965647360C501593827 @default.
- W1965647360 hasConceptScore W1965647360C521977710 @default.
- W1965647360 hasConceptScore W1965647360C547475151 @default.
- W1965647360 hasConceptScore W1965647360C55493867 @default.
- W1965647360 hasConceptScore W1965647360C71240020 @default.
- W1965647360 hasConceptScore W1965647360C94412978 @default.
- W1965647360 hasConceptScore W1965647360C94665300 @default.