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- W1965658506 abstract "It was the objective of this paper to assess the applicability of the universal calibration method to aqueous GPC/SEC with nonionic and anionic polymers using the Viscotek differential viscosity detector. Three water-soluble polymers—polyacrylic acid, dextran, and polyethylene oxide—were chromatographed using four UltrahydrogelTM columns with 0.3 M NaCl and 0.1M KH2PO4 as the mobile phase adjusted to pH 7. Three distinct calibration curves were obtained. Upon addition of 10% methanol, a reasonably good universal calibration curve was obtained. However, quantitative analysis of the data exhibited about 5% deviation in average Mw and Mn for sodium polyacrylate as calculated from the single curve as opposed to about 40% when calculated from the composite curve. The applicability of three theoretical models for the universal calibration method was assessed, and a recommendation was made for future work. © 1993 John Wiley & Sons, Inc." @default.
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- W1965658506 date "1993-06-05" @default.
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- W1965658506 title "Universal calibration assessment in aqueous gel permeation chromatography" @default.
- W1965658506 doi "https://doi.org/10.1002/app.1993.070480904" @default.
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