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- W1965714489 abstract "Abstract Background: The Precision ® (Abbott Diabetes Care) point-of-care biosensor test strips are widely used by patients with diabetes and clinical laboratories for measurement of plasma β-hydroxybutyrate (β-HB) concentrations in capillary blood samples obtained by fingerstick. In the literature, this procedure has been validated only against the enzymatic determination of β-HB in venous plasma, i.e., the method to which the Precision ® has been calibrated. Methods: In this study, the Precision ® Xceed was compared to a methodologically different and superior procedure: determination of β-HB by liquid chromatography tandem-mass spectrometry (LC-MS/MS) in capillary blood spots. Blood spots were obtained from the same fingerstick sample from out of which Precision ® measurements were performed. Linearity was tested by adding varying amounts of standard to an EDTA venous whole blood matrix. Results: The Precision ® was in good agreement with LC-MS/MS within the measuring range of 0.0–6.0 mmol/L (Passing and Bablok regression: slope=1.20 and no significant intercept, R=0.97, n=59). Surprisingly, the Precision ® showed non-linearity and full saturation at concentrations above 6.0 mmol/L, which were confirmed by a standard addition experiment. Results obtained at the saturation level varied between 3.0 and 6.5 mmol/L. Conclusions: The Precision ® β-HB test strips demonstrate good comparison with LC-MS/MS. Inter-individual variation around the saturation level, however, is large. Therefore, we advise reporting readings above 3.0 as >3.0 mmol/L. The test is valid for use in the clinically relevant range of 0.0–3.0 mmol/L. Clin Chem Lab Med 2010;48:1781–4." @default.
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- W1965714489 date "2010-08-24" @default.
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- W1965714489 title "Accuracy of the Precision<sup>®</sup> point-of-care ketone test examined by liquid chromatography tandem-mass spectrometry (LC-MS/MS) in the same fingerstick sample" @default.
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- W1965714489 doi "https://doi.org/10.1515/cclm.2010.351" @default.
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