FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965750072> ?p ?o ?g. }

• W1965750072 endingPage "67" @default.
• W1965750072 startingPage "55" @default.
• W1965750072 abstract "A method of reverse transcription followed by polymerase chain reaction (RT-PCR) has been implemented for the demonstration of the rabbit haemorrhagic disease virus (RHDV) genome in organ suspensions, leukocytes and excretions of infected rabbits. RT-PCR has been tested with 10 RHDV strains isolated at various geographic sites and times using a pair of primers coming from the gene region coding for the capsid protein VP60. The same primers were effective in the amplification of 4 of 5 European brown hare syndrome (EBHS) virus isolates. Non-radioactive labelling of PCR products with digoxigenin during the amplification and a system of colorimetric assessment of hybridization reactions between a biotin-labelled RHDV capture probe and the chains of labelled amplicons (PCR ELISA) were used for specific analyses of nucleic acid synthesis. The sensitivity of the alternative procedure of analysis of the dig-labelled PCR products with PCR ELISA was two logs10 higher than that of conventional electrophoresis in agarose gel stained with ethidium bromide. The results of the hybridization reactions, carried out under various stringency conditions, have confirmed the presumption that the genomic similarity between the amplified and the probed areas of the capsid protein VP60 gene was not uniform within all the tested caliciviruses. A higher degree of heterogeneity was observed between the isolates of EBHSV and RHDV." @default.
• W1965750072 created "2016-06-24" @default.
• W1965750072 creator A5036392698 @default.
• W1965750072 creator A5040671910 @default.
• W1965750072 creator A5049843732 @default.
• W1965750072 creator A5051559946 @default.
• W1965750072 creator A5051678827 @default.
• W1965750072 creator A5085651316 @default.
• W1965750072 date "1997-06-01" @default.
• W1965750072 modified "2023-10-14" @default.
• W1965750072 title "Colorimetric detection of lagomorphs' calicivirus genomic sequences by polymerase chain reaction incorporating digoxigenin dUTP" @default.
• W1965750072 cites W1515488444 @default.
• W1965750072 cites W1953716325 @default.
• W1965750072 cites W1969612079 @default.
• W1965750072 cites W1975821332 @default.
• W1965750072 cites W1987650603 @default.
• W1965750072 cites W2011691385 @default.
• W1965750072 cites W2015127138 @default.
• W1965750072 cites W2034777115 @default.
• W1965750072 cites W2055167983 @default.
• W1965750072 cites W2059224121 @default.
• W1965750072 cites W2072830066 @default.
• W1965750072 cites W2103777819 @default.
• W1965750072 cites W2124086379 @default.
• W1965750072 cites W2130775348 @default.
• W1965750072 cites W2166229810 @default.
• W1965750072 cites W2183068221 @default.
• W1965750072 cites W2407560572 @default.
• W1965750072 cites W2444602779 @default.
• W1965750072 cites W2468658448 @default.
• W1965750072 cites W2475714682 @default.
• W1965750072 cites W2500377989 @default.
• W1965750072 cites W2759698975 @default.
• W1965750072 cites W2989345970 @default.
• W1965750072 cites W3042691651 @default.
• W1965750072 doi "https://doi.org/10.1016/s0378-1135(96)01348-x" @default.
• W1965750072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9231981" @default.
• W1965750072 hasPublicationYear "1997" @default.
• W1965750072 type Work @default.
• W1965750072 sameAs 1965750072 @default.
• W1965750072 citedByCount "8" @default.
• W1965750072 countsByYear W19657500722012 @default.
• W1965750072 countsByYear W19657500722019 @default.
• W1965750072 countsByYear W19657500722021 @default.
• W1965750072 crossrefType "journal-article" @default.
• W1965750072 hasAuthorship W1965750072A5036392698 @default.
• W1965750072 hasAuthorship W1965750072A5040671910 @default.
• W1965750072 hasAuthorship W1965750072A5049843732 @default.
• W1965750072 hasAuthorship W1965750072A5051559946 @default.
• W1965750072 hasAuthorship W1965750072A5051678827 @default.
• W1965750072 hasAuthorship W1965750072A5085651316 @default.
• W1965750072 hasConcept C104317684 @default.
• W1965750072 hasConcept C150194340 @default.
• W1965750072 hasConcept C153911025 @default.
• W1965750072 hasConcept C159047783 @default.
• W1965750072 hasConcept C17757408 @default.
• W1965750072 hasConcept C202878990 @default.
• W1965750072 hasConcept C2522874641 @default.
• W1965750072 hasConcept C2776552150 @default.
• W1965750072 hasConcept C2777747160 @default.
• W1965750072 hasConcept C2778271344 @default.
• W1965750072 hasConcept C2778476535 @default.
• W1965750072 hasConcept C2778750513 @default.
• W1965750072 hasConcept C49105822 @default.
• W1965750072 hasConcept C54355233 @default.
• W1965750072 hasConcept C81439078 @default.
• W1965750072 hasConcept C8185291 @default.
• W1965750072 hasConcept C86803240 @default.
• W1965750072 hasConceptScore W1965750072C104317684 @default.
• W1965750072 hasConceptScore W1965750072C150194340 @default.
• W1965750072 hasConceptScore W1965750072C153911025 @default.
• W1965750072 hasConceptScore W1965750072C159047783 @default.
• W1965750072 hasConceptScore W1965750072C17757408 @default.
• W1965750072 hasConceptScore W1965750072C202878990 @default.
• W1965750072 hasConceptScore W1965750072C2522874641 @default.
• W1965750072 hasConceptScore W1965750072C2776552150 @default.
• W1965750072 hasConceptScore W1965750072C2777747160 @default.
• W1965750072 hasConceptScore W1965750072C2778271344 @default.
• W1965750072 hasConceptScore W1965750072C2778476535 @default.
• W1965750072 hasConceptScore W1965750072C2778750513 @default.
• W1965750072 hasConceptScore W1965750072C49105822 @default.
• W1965750072 hasConceptScore W1965750072C54355233 @default.
• W1965750072 hasConceptScore W1965750072C81439078 @default.
• W1965750072 hasConceptScore W1965750072C8185291 @default.
• W1965750072 hasConceptScore W1965750072C86803240 @default.
• W1965750072 hasIssue "1" @default.
• W1965750072 hasLocation W19657500721 @default.
• W1965750072 hasLocation W19657500722 @default.
• W1965750072 hasOpenAccess W1965750072 @default.
• W1965750072 hasPrimaryLocation W19657500721 @default.
• W1965750072 hasRelatedWork W1658688221 @default.
• W1965750072 hasRelatedWork W1793505562 @default.
• W1965750072 hasRelatedWork W1965750072 @default.
• W1965750072 hasRelatedWork W2018168151 @default.
• W1965750072 hasRelatedWork W2039540399 @default.
• W1965750072 hasRelatedWork W2047106367 @default.
• W1965750072 hasRelatedWork W2381738859 @default.
• W1965750072 hasRelatedWork W3045089328 @default.

• next