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- W1965798843 abstract "Secretion of microneme proteins is essential to Plasmodium invasion but the molecular composition of these secretory organelles remains poorly defined. Here, we describe the first Plasmodium microneme proteome. Purification of micronemes by subcellular fractionation from cultured ookinetes was confirmed by enrichment of known micronemal proteins and electron microscopy. Quantitation of electron micrographs showed >14-fold microneme enrichment compared to the intact ookinete, such that micronemes comprised 85% of the identifiable organelles in the fraction. Gel LC-MS/MS of the most abundant protein constituents of the fraction identified three known micronemal proteins chitinase, CTRP, SOAP, together with protein disulphide isomerase (PDI) and HSP70. Highly sensitive MudPIT shotgun proteomics described a total of 345 proteins in the fraction. M1 aminopeptidase and PDI, the former a recognised target of drug development, were both shown to have a micronemal location by IFA. We further identified numerous proteins with established vesicle trafficking and signaling functions consistent with micronemes being part of a regulated secretory pathway. Previously uncharacterised proteins comprise the largest functional group of the microneme proteome and will include secreted proteins important to invasion." @default.
- W1965798843 created "2016-06-24" @default.
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- W1965798843 date "2009-03-01" @default.
- W1965798843 modified "2023-09-23" @default.
- W1965798843 title "Characterisation of Plasmodium invasive organelles; an ookinete microneme proteome" @default.
- W1965798843 cites W1525575010 @default.
- W1965798843 cites W1597678601 @default.
- W1965798843 cites W1604018212 @default.
- W1965798843 cites W1818853492 @default.
- W1965798843 cites W1879579138 @default.
- W1965798843 cites W1905994259 @default.
- W1965798843 cites W1967020388 @default.
- W1965798843 cites W1967910146 @default.
- W1965798843 cites W1974245401 @default.
- W1965798843 cites W1974587245 @default.
- W1965798843 cites W1974944488 @default.
- W1965798843 cites W1975356184 @default.
- W1965798843 cites W1977648288 @default.
- W1965798843 cites W1977964734 @default.
- W1965798843 cites W1981214208 @default.
- W1965798843 cites W1983764955 @default.
- W1965798843 cites W1985239826 @default.
- W1965798843 cites W1989309678 @default.
- W1965798843 cites W1993973406 @default.
- W1965798843 cites W1997504014 @default.
- W1965798843 cites W1997623795 @default.
- W1965798843 cites W1998190883 @default.
- W1965798843 cites W1998525345 @default.
- W1965798843 cites W1998834739 @default.
- W1965798843 cites W2003843685 @default.
- W1965798843 cites W2006963069 @default.
- W1965798843 cites W2012932145 @default.
- W1965798843 cites W2013410321 @default.
- W1965798843 cites W2015221103 @default.
- W1965798843 cites W2018581247 @default.
- W1965798843 cites W2019211309 @default.
- W1965798843 cites W2026465178 @default.
- W1965798843 cites W2027990366 @default.
- W1965798843 cites W2028704597 @default.
- W1965798843 cites W2029052776 @default.
- W1965798843 cites W2029553332 @default.
- W1965798843 cites W2031966712 @default.
- W1965798843 cites W2032554631 @default.
- W1965798843 cites W2040110410 @default.
- W1965798843 cites W2047308369 @default.
- W1965798843 cites W2048171163 @default.
- W1965798843 cites W2052767941 @default.
- W1965798843 cites W2062963197 @default.
- W1965798843 cites W2064230518 @default.
- W1965798843 cites W2064562674 @default.
- W1965798843 cites W2077387356 @default.
- W1965798843 cites W2080412532 @default.
- W1965798843 cites W2084047564 @default.
- W1965798843 cites W2088298861 @default.
- W1965798843 cites W2088890918 @default.
- W1965798843 cites W2099872182 @default.
- W1965798843 cites W2102431991 @default.
- W1965798843 cites W2103819628 @default.
- W1965798843 cites W2104847338 @default.
- W1965798843 cites W2112017417 @default.
- W1965798843 cites W2112657291 @default.
- W1965798843 cites W2112905569 @default.
- W1965798843 cites W2112925184 @default.
- W1965798843 cites W2114241062 @default.
- W1965798843 cites W2114758077 @default.
- W1965798843 cites W2118013877 @default.
- W1965798843 cites W2118103211 @default.
- W1965798843 cites W2121624499 @default.
- W1965798843 cites W2122910625 @default.
- W1965798843 cites W2123382925 @default.
- W1965798843 cites W2129895984 @default.
- W1965798843 cites W2135337307 @default.
- W1965798843 cites W2137917513 @default.
- W1965798843 cites W2138439665 @default.
- W1965798843 cites W2142044718 @default.
- W1965798843 cites W2143304554 @default.
- W1965798843 cites W2145586756 @default.
- W1965798843 cites W2145835877 @default.
- W1965798843 cites W2149331907 @default.
- W1965798843 cites W2151497677 @default.
- W1965798843 cites W2156022009 @default.
- W1965798843 cites W2161746138 @default.
- W1965798843 cites W2164908456 @default.
- W1965798843 cites W2167188257 @default.
- W1965798843 cites W2406760893 @default.
- W1965798843 doi "https://doi.org/10.1002/pmic.200800404" @default.
- W1965798843 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2706521" @default.
- W1965798843 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19206106" @default.
- W1965798843 hasPublicationYear "2009" @default.
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