Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965809741> ?p ?o ?g. }
- W1965809741 endingPage "99" @default.
- W1965809741 startingPage "87" @default.
- W1965809741 abstract "A neuroblastoma × glioma hybrid cell line, NG108-15, was able to induce the aggregation of AChRs when co-cultured with myotubes. NG108-15 cells in culture expressed agrin, producing a protein of ≈220 kDa and a transcript of ≈8.0 kb. The mRNA encoding the agrin isoform having no amino acid insertion at either the Y or the Z site, namely agrin0,0, was the only transcript detected in NG108-15 cells when they were cultured alone or co-cultured with myotubes. NG108-15 cells could be induced to differentiate by chemical treatment, and the chemical-induced differentiation of NG108-15 cells increased the level of agrin mRNA expression approximately fourfold while the expression of a housekeeping gene remained relatively unchanged. The increase in agrin expression of differentiated NG108-15 cells paralleled the increase in AChR-aggregating activity of differentiated NG108-15 cells, indicating that the agrin derived from NG108-15 cells could be the receptor-aggregating factor. In addition, we created a stable clonal NG108-15 cell line that was transfected with antisense agrin cDNA and its expression of agrin was abolished, while its AChR-aggregating activity was completely lost when co-cultured with myotubes. This is the first direct demonstration that NG108-15 cell-induced AChR aggregation on cultured myotubes is mediated by neuron-derived agrin." @default.
- W1965809741 created "2016-06-24" @default.
- W1965809741 creator A5031630099 @default.
- W1965809741 creator A5055673163 @default.
- W1965809741 date "1997-01-01" @default.
- W1965809741 modified "2023-10-18" @default.
- W1965809741 title "Antisense Agrin cDNA Transfection Blocks Neuroblastoma Cell-Induced Acetylcholine Receptor Aggregation When Co-cultured with Myotubes" @default.
- W1965809741 cites W1516158645 @default.
- W1965809741 cites W1869842964 @default.
- W1965809741 cites W1947484022 @default.
- W1965809741 cites W1967918713 @default.
- W1965809741 cites W1976805375 @default.
- W1965809741 cites W1981185926 @default.
- W1965809741 cites W1985247859 @default.
- W1965809741 cites W1987098829 @default.
- W1965809741 cites W1992157271 @default.
- W1965809741 cites W1999880438 @default.
- W1965809741 cites W2001630331 @default.
- W1965809741 cites W2008256279 @default.
- W1965809741 cites W2016828215 @default.
- W1965809741 cites W2019960246 @default.
- W1965809741 cites W2027995984 @default.
- W1965809741 cites W2038866255 @default.
- W1965809741 cites W2044199827 @default.
- W1965809741 cites W2046678465 @default.
- W1965809741 cites W2047428077 @default.
- W1965809741 cites W2050748185 @default.
- W1965809741 cites W2055325893 @default.
- W1965809741 cites W2058698951 @default.
- W1965809741 cites W2058966822 @default.
- W1965809741 cites W2065688400 @default.
- W1965809741 cites W2068836997 @default.
- W1965809741 cites W2070534094 @default.
- W1965809741 cites W2089011655 @default.
- W1965809741 cites W2090390636 @default.
- W1965809741 cites W2090473949 @default.
- W1965809741 cites W2094689830 @default.
- W1965809741 cites W2096571156 @default.
- W1965809741 cites W2113222492 @default.
- W1965809741 cites W2132776141 @default.
- W1965809741 cites W2139650552 @default.
- W1965809741 cites W2148525477 @default.
- W1965809741 cites W2155080404 @default.
- W1965809741 cites W2156080044 @default.
- W1965809741 cites W2165356341 @default.
- W1965809741 cites W4293247451 @default.
- W1965809741 cites W4294216491 @default.
- W1965809741 doi "https://doi.org/10.1006/mcne.1997.0637" @default.
- W1965809741 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9361290" @default.
- W1965809741 hasPublicationYear "1997" @default.
- W1965809741 type Work @default.
- W1965809741 sameAs 1965809741 @default.
- W1965809741 citedByCount "22" @default.
- W1965809741 countsByYear W19658097412012 @default.
- W1965809741 countsByYear W19658097412018 @default.
- W1965809741 crossrefType "journal-article" @default.
- W1965809741 hasAuthorship W1965809741A5031630099 @default.
- W1965809741 hasAuthorship W1965809741A5055673163 @default.
- W1965809741 hasConcept C104317684 @default.
- W1965809741 hasConcept C105580179 @default.
- W1965809741 hasConcept C150194340 @default.
- W1965809741 hasConcept C153911025 @default.
- W1965809741 hasConcept C170493617 @default.
- W1965809741 hasConcept C187882448 @default.
- W1965809741 hasConcept C207200792 @default.
- W1965809741 hasConcept C2779024559 @default.
- W1965809741 hasConcept C3763915 @default.
- W1965809741 hasConcept C54009773 @default.
- W1965809741 hasConcept C54355233 @default.
- W1965809741 hasConcept C55493867 @default.
- W1965809741 hasConcept C80161118 @default.
- W1965809741 hasConcept C81885089 @default.
- W1965809741 hasConcept C86803240 @default.
- W1965809741 hasConcept C95444343 @default.
- W1965809741 hasConceptScore W1965809741C104317684 @default.
- W1965809741 hasConceptScore W1965809741C105580179 @default.
- W1965809741 hasConceptScore W1965809741C150194340 @default.
- W1965809741 hasConceptScore W1965809741C153911025 @default.
- W1965809741 hasConceptScore W1965809741C170493617 @default.
- W1965809741 hasConceptScore W1965809741C187882448 @default.
- W1965809741 hasConceptScore W1965809741C207200792 @default.
- W1965809741 hasConceptScore W1965809741C2779024559 @default.
- W1965809741 hasConceptScore W1965809741C3763915 @default.
- W1965809741 hasConceptScore W1965809741C54009773 @default.
- W1965809741 hasConceptScore W1965809741C54355233 @default.
- W1965809741 hasConceptScore W1965809741C55493867 @default.
- W1965809741 hasConceptScore W1965809741C80161118 @default.
- W1965809741 hasConceptScore W1965809741C81885089 @default.
- W1965809741 hasConceptScore W1965809741C86803240 @default.
- W1965809741 hasConceptScore W1965809741C95444343 @default.
- W1965809741 hasIssue "1-2" @default.
- W1965809741 hasLocation W19658097411 @default.
- W1965809741 hasLocation W19658097412 @default.
- W1965809741 hasOpenAccess W1965809741 @default.
- W1965809741 hasPrimaryLocation W19658097411 @default.
- W1965809741 hasRelatedWork W1969691270 @default.
- W1965809741 hasRelatedWork W1972066333 @default.
- W1965809741 hasRelatedWork W2029119694 @default.