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• W1965817524 startingPage "4674" @default.
• W1965817524 abstract "NMR investigations have been carried out on the B1 domain of protein G. This protein has six lysine residues, of which three are consistently found to form surface-exposed salt bridges in crystal structures, while the other three are not. The Nζ and Hζ chemical shifts of all six lysines are similar and are not affected significantly by pH titration of the carboxylate groups in the protein, except for a relatively small titration of K39 Nζ. Deuterium isotope effects on nitrogen and proton are of the size expected for a simple hydrated amine (a result supported by density functional theory calculations), and also do not titrate with the carboxylates. The line shapes of the J-coupled 15N signals suggest rapid internal reorientation of all NH3+ groups. pKa values have been measured for all charged side chains except Glu50 and do not show the perturbations expected for salt bridge formation, except that E35 has a Hill coefficient of 0.84. The main differential effect seen is that the lysines that are involved in salt bridges in the crystal display faster exchange of the amine protons with the solvent, an effect attributed to general base catalysis by the carboxylates. This explanation is supported by varying buffer composition, which demonstrates reduced electrostatic shielding at low concentration. In conclusion, the study demonstrates that the six surface-exposed lysines in protein G are not involved in significant salt bridge interactions, even though such interactions are found consistently in crystal structures. However, the intrahelical E35−K39 (i,i+4) interaction is partially present." @default.
• W1965817524 created "2016-06-24" @default.
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• W1965817524 date "2009-03-12" @default.
• W1965817524 modified "2023-10-10" @default.
• W1965817524 title "Characterization of Salt Bridges to Lysines in the Protein G B1 Domain" @default.
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• W1965817524 doi "https://doi.org/10.1021/ja808223p" @default.
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