Matches in SemOpenAlex for { <https://semopenalex.org/work/W1965936614> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W1965936614 endingPage "85" @default.
- W1965936614 startingPage "79" @default.
- W1965936614 abstract "The procedure of centrifugal elutriation was evaluated as a means of purifying large numbers of in situ lymphocytes from enzymatically disaggregated mouse mammary tumors. The eluate obtained at a flow rate of 3.0 ml/min was optimal for high levels of lymphocyte recovery with low levels of contaminating tumor cells, polymorphonuclear leukocytes, and macrophages. The majority of the tumor infiltrating lymphocytes (90%) expressed the Thy 1.2 antigen, while less than 5% possessed surface immunoglobulin. Further analysis of the T lymphocyte population was accomplished by flow cytometric analysis of in situ lymphocytes stained with fluorescein-conjugated monoclonal anti-Lyt 2 antibodies. The results of such studies reveal an increase in the levels of Lyt 2+ lymphocytes within the in situ population. To determine whether these Lyt 2+ cells were functionally active as suppressor cells, the ISL1 were mixed with spleen cells from tumor bearers and then tested for their ability to respond to mitogen and TAA-induced blast transformation of tumor-bearer spleen cells. Removal of macrophages from ISL by Sephadex G-10 columns did not alter the suppression. Treatment with monoclonal anti-Lyt 1 antibody and complement did not affect the inhibition observed. However, treatment of ISL with anti-Lyt 2+ monoclonal antibody and complement resulted in the elimination of the suppressor cell activity. We concluded that within the tumor-infiltrating lymphoreticular cells there is a population of Thy 1.2+ Lyt 2.2+ lymphocytes responsible for the suppression of mitogen and tumor-antigen-induced blastogenesis." @default.
- W1965936614 created "2016-06-24" @default.
- W1965936614 creator A5011033391 @default.
- W1965936614 creator A5034893325 @default.
- W1965936614 creator A5043275002 @default.
- W1965936614 creator A5066238218 @default.
- W1965936614 date "1984-01-15" @default.
- W1965936614 modified "2023-10-17" @default.
- W1965936614 title "Lymphoreticular cells isolated by centrifugal elutriation from a mammary adenocaricinoma. i. characterization of anin situ lymphocyte suppressor population by surface markers and functional reactivity" @default.
- W1965936614 cites W1980181948 @default.
- W1965936614 cites W1992279805 @default.
- W1965936614 cites W2005770085 @default.
- W1965936614 cites W2010682831 @default.
- W1965936614 cites W2012095859 @default.
- W1965936614 cites W2018751787 @default.
- W1965936614 cites W2040861149 @default.
- W1965936614 cites W2059164565 @default.
- W1965936614 cites W2059273877 @default.
- W1965936614 cites W2084648417 @default.
- W1965936614 cites W2104453235 @default.
- W1965936614 cites W2141577010 @default.
- W1965936614 cites W2343541789 @default.
- W1965936614 doi "https://doi.org/10.1002/ijc.2910330114" @default.
- W1965936614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6229495" @default.
- W1965936614 hasPublicationYear "1984" @default.
- W1965936614 type Work @default.
- W1965936614 sameAs 1965936614 @default.
- W1965936614 citedByCount "16" @default.
- W1965936614 crossrefType "journal-article" @default.
- W1965936614 hasAuthorship W1965936614A5011033391 @default.
- W1965936614 hasAuthorship W1965936614A5034893325 @default.
- W1965936614 hasAuthorship W1965936614A5043275002 @default.
- W1965936614 hasAuthorship W1965936614A5066238218 @default.
- W1965936614 hasConcept C147483822 @default.
- W1965936614 hasConcept C153911025 @default.
- W1965936614 hasConcept C159654299 @default.
- W1965936614 hasConcept C203014093 @default.
- W1965936614 hasConcept C2777761686 @default.
- W1965936614 hasConcept C2780931953 @default.
- W1965936614 hasConcept C2908647359 @default.
- W1965936614 hasConcept C2992881475 @default.
- W1965936614 hasConcept C542903549 @default.
- W1965936614 hasConcept C71924100 @default.
- W1965936614 hasConcept C86803240 @default.
- W1965936614 hasConcept C99454951 @default.
- W1965936614 hasConceptScore W1965936614C147483822 @default.
- W1965936614 hasConceptScore W1965936614C153911025 @default.
- W1965936614 hasConceptScore W1965936614C159654299 @default.
- W1965936614 hasConceptScore W1965936614C203014093 @default.
- W1965936614 hasConceptScore W1965936614C2777761686 @default.
- W1965936614 hasConceptScore W1965936614C2780931953 @default.
- W1965936614 hasConceptScore W1965936614C2908647359 @default.
- W1965936614 hasConceptScore W1965936614C2992881475 @default.
- W1965936614 hasConceptScore W1965936614C542903549 @default.
- W1965936614 hasConceptScore W1965936614C71924100 @default.
- W1965936614 hasConceptScore W1965936614C86803240 @default.
- W1965936614 hasConceptScore W1965936614C99454951 @default.
- W1965936614 hasIssue "1" @default.
- W1965936614 hasLocation W19659366141 @default.
- W1965936614 hasLocation W19659366142 @default.
- W1965936614 hasOpenAccess W1965936614 @default.
- W1965936614 hasPrimaryLocation W19659366141 @default.
- W1965936614 hasRelatedWork W1943797027 @default.
- W1965936614 hasRelatedWork W1997716688 @default.
- W1965936614 hasRelatedWork W2037509311 @default.
- W1965936614 hasRelatedWork W2038126255 @default.
- W1965936614 hasRelatedWork W2064089409 @default.
- W1965936614 hasRelatedWork W2068190618 @default.
- W1965936614 hasRelatedWork W2087539308 @default.
- W1965936614 hasRelatedWork W2368460920 @default.
- W1965936614 hasRelatedWork W2380360044 @default.
- W1965936614 hasRelatedWork W2417789235 @default.
- W1965936614 hasVolume "33" @default.
- W1965936614 isParatext "false" @default.
- W1965936614 isRetracted "false" @default.
- W1965936614 magId "1965936614" @default.
- W1965936614 workType "article" @default.