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• W1965970344 startingPage "3026" @default.
• W1965970344 abstract "The presence of Escherichia coli (E. coli) in food and drinking water is a chronic problem worldwide. Protecting food against bacterial contamination and rapid diagnosis of infection require simple and rapid assays for detection of bacterial pathogens, including E. coli O157:H7. Here we report a rapid and novel colorimetric method for detecting E. coli O157:H7. This colorimetric method is based on the catalytic oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine by hydrogen peroxide using 4-mercaptophenylboronic acid-functioned Au@Pt nanoparticles adsorbed on the surface of E. coli O157:H7. The assay showed excellent sensitivity both with the naked eye and based on absorbance measurements. The absorbance at 652 nm was proportional to the concentration of E. coli O157:H7 ranging from 7 to 6 × 10(6) cfu mL(-1) with a limit of detection of 7 cfu mL(-1). The total detection time was less than 40 min." @default.
• W1965970344 created "2016-06-24" @default.
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• W1965970344 date "2013-01-01" @default.
• W1965970344 modified "2023-10-02" @default.
• W1965970344 title "Colorimetric detection of Escherichia coli O157:H7 using functionalized Au@Pt nanoparticles as peroxidase mimetics" @default.
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• W1965970344 doi "https://doi.org/10.1039/c3an00026e" @default.
• W1965970344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23577341" @default.
• W1965970344 hasPublicationYear "2013" @default.
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