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• W1965983407 abstract "β-Mannosidase (EC 3.2.1.25) is an exoglycosidase specific for the hydrolysis of terminal β-linked mannoside in various sugar chains. cDNA corresponding to the β-mannosidase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Pichia pastoris. The β-mannosidase gene contains an open reading frame which encodes the protein with 933 amino acid residues. The wild type and recombinant proteins were purified to apparent homogeneity and biochemically characterized (K(M) 0.28 and 0.44 mmol/l for p-nitrophenyl β-d-mannopyranoside, pI 4.2 and 4.0, and their pH optima were at pH 4.5 and 5.5 and 65°C, respectively)." @default.
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• W1965983407 date "2012-10-01" @default.
• W1965983407 modified "2023-10-17" @default.
• W1965983407 title "Production of Aspergillus niger β-mannosidase in Pichia pastoris" @default.
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• W1965983407 doi "https://doi.org/10.1016/j.pep.2012.07.012" @default.
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