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- W1966036103 abstract "A procedure is described for detecting D-ribose 5-phosphate ketol isomerase after electrophoresis, using ribose 5-phosphate as a substrate and alkaline 3:4-dinitrobenzoic acid to reveal the ribulose 5-phosphate formed. The enzyme was assayed by two different methods in the supernatant fraction of rat tissues and two electrophoretic forms were found in spleen, small intestine, uterus, heart muscle, and brain. Only one form was detected in lung, blood, kidney, and liver, while three forms were found in an extract of spinach. A partial separation of the two forms in rat spleen was achieved by chromatography on DEAE-Sephadex A-50. However, both these forms and the single form in rat liver had similar Km values lying between 0.28 and 0.39 mM D-ribose 5-phosphate." @default.
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- W1966036103 date "1974-01-01" @default.
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- W1966036103 title "The detection and occurrence of multiple forms of D-ribose 5-phosphate ketol isomerase" @default.
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- W1966036103 doi "https://doi.org/10.1016/s0003-9861(74)80005-6" @default.
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