FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966067794> ?p ?o ?g. }

• W1966067794 endingPage "144" @default.
• W1966067794 startingPage "134" @default.
• W1966067794 abstract "Apidaecins represent an important group of antimicrobial peptides occurring in honey bee hemolymph, where they play an important role as key components of humoral immunity. The present study demonstrates the development of a highly sensitive assay for apidaecin 1 isoforms quantification in the hemolymph or body parts from honey bee individuals. The analytical protocol comprises apidaecins 1 purification and enrichment steps by weak cation-exchange chromatography (WCX) in laboratory-made WCX-Tip microcolumns combined with a desalting step on a reversed-phase sorbent (C8) carried in StageTips. Apidaecin-enriched fraction was analyzed by a reversed-phase based nanoliquid chromatography (C4) separation coupled with high-resolution mass spectrometry. The method performance was validated in its specificity, linearity (0-5pmol), recovery (∼45%), precision (<10% at 0.1pmol), limit of detection (∼50fmol), limit of quantification (0.1pmol) and sample stability. The method was successfully applied to analyze the content of apidaecin 1 isoforms in the following samples: hemolymph - 13.0ng/μL (95% confidence interval of 7.5-18.6ng/μL), thoraxes - 36.2ng/unit (95% CI of 18.9-53.6ng/unit) and heads - 12.9ng/unit (95% CI of 9.1-16.7ng/unit). Freshly emerged bees had apidaecin 1 isoforms levels below the limit of detection. Thus it was possible to use them as a competitive matrix for calibration standards to prevent losses of highly basic apidaecins. This new protocol for apidaecin 1 isoforms quantification represents a promising tool to study the role of apidaecins in honey bee immunity and can be considered as a proof-of-concept for the development of sensitive quantification methods for basic antimicrobial peptides in various organisms." @default.
• W1966067794 created "2016-06-24" @default.
• W1966067794 creator A5038330203 @default.
• W1966067794 creator A5042183780 @default.
• W1966067794 creator A5051710329 @default.
• W1966067794 creator A5076596907 @default.
• W1966067794 date "2014-12-01" @default.
• W1966067794 modified "2023-09-28" @default.
• W1966067794 title "A sensitive quantification of the peptide apidaecin 1 isoforms in single bee tissues using a weak cation exchange pre-separation and nanocapillary liquid chromatography coupled with mass spectrometry" @default.
• W1966067794 cites W144997804 @default.
• W1966067794 cites W1514331928 @default.
• W1966067794 cites W165518954 @default.
• W1966067794 cites W1964064121 @default.
• W1966067794 cites W1966939855 @default.
• W1966067794 cites W1970116840 @default.
• W1966067794 cites W197620025 @default.
• W1966067794 cites W1980584731 @default.
• W1966067794 cites W1986388841 @default.
• W1966067794 cites W1986656413 @default.
• W1966067794 cites W1988180248 @default.
• W1966067794 cites W1998351766 @default.
• W1966067794 cites W2000798961 @default.
• W1966067794 cites W2003236418 @default.
• W1966067794 cites W2003587139 @default.
• W1966067794 cites W2013278992 @default.
• W1966067794 cites W2015398987 @default.
• W1966067794 cites W2017694438 @default.
• W1966067794 cites W2035758756 @default.
• W1966067794 cites W2037259497 @default.
• W1966067794 cites W2040530229 @default.
• W1966067794 cites W2042278775 @default.
• W1966067794 cites W2049787302 @default.
• W1966067794 cites W2051701435 @default.
• W1966067794 cites W2056502023 @default.
• W1966067794 cites W2064643160 @default.
• W1966067794 cites W2068620810 @default.
• W1966067794 cites W2069475240 @default.
• W1966067794 cites W2070695415 @default.
• W1966067794 cites W2073528755 @default.
• W1966067794 cites W2078370711 @default.
• W1966067794 cites W2079256027 @default.
• W1966067794 cites W2079627389 @default.
• W1966067794 cites W2085021557 @default.
• W1966067794 cites W2087363058 @default.
• W1966067794 cites W2089110154 @default.
• W1966067794 cites W2089559505 @default.
• W1966067794 cites W2092014341 @default.
• W1966067794 cites W2092285264 @default.
• W1966067794 cites W2094389762 @default.
• W1966067794 cites W2105167594 @default.
• W1966067794 cites W2106376891 @default.
• W1966067794 cites W2107116365 @default.
• W1966067794 cites W2113766156 @default.
• W1966067794 cites W2122183448 @default.
• W1966067794 cites W2123715695 @default.
• W1966067794 cites W2145274401 @default.
• W1966067794 cites W2151670363 @default.
• W1966067794 cites W2154740780 @default.
• W1966067794 cites W2474396486 @default.
• W1966067794 cites W4293247451 @default.
• W1966067794 cites W6098071 @default.
• W1966067794 doi "https://doi.org/10.1016/j.chroma.2014.11.041" @default.
• W1966067794 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25435459" @default.
• W1966067794 hasPublicationYear "2014" @default.
• W1966067794 type Work @default.
• W1966067794 sameAs 1966067794 @default.
• W1966067794 citedByCount "5" @default.
• W1966067794 countsByYear W19660677942015 @default.
• W1966067794 countsByYear W19660677942017 @default.
• W1966067794 countsByYear W19660677942018 @default.
• W1966067794 countsByYear W19660677942020 @default.
• W1966067794 crossrefType "journal-article" @default.
• W1966067794 hasAuthorship W1966067794A5038330203 @default.
• W1966067794 hasAuthorship W1966067794A5042183780 @default.
• W1966067794 hasAuthorship W1966067794A5051710329 @default.
• W1966067794 hasAuthorship W1966067794A5076596907 @default.
• W1966067794 hasConcept C100068826 @default.
• W1966067794 hasConcept C119128265 @default.
• W1966067794 hasConcept C162356407 @default.
• W1966067794 hasConcept C185592680 @default.
• W1966067794 hasConcept C43617362 @default.
• W1966067794 hasConcept C55493867 @default.
• W1966067794 hasConceptScore W1966067794C100068826 @default.
• W1966067794 hasConceptScore W1966067794C119128265 @default.
• W1966067794 hasConceptScore W1966067794C162356407 @default.
• W1966067794 hasConceptScore W1966067794C185592680 @default.
• W1966067794 hasConceptScore W1966067794C43617362 @default.
• W1966067794 hasConceptScore W1966067794C55493867 @default.
• W1966067794 hasFunder F4320321005 @default.
• W1966067794 hasLocation W19660677941 @default.
• W1966067794 hasLocation W19660677942 @default.
• W1966067794 hasOpenAccess W1966067794 @default.
• W1966067794 hasPrimaryLocation W19660677941 @default.
• W1966067794 hasRelatedWork W1950305724 @default.
• W1966067794 hasRelatedWork W1976257423 @default.
• W1966067794 hasRelatedWork W1977440618 @default.
• W1966067794 hasRelatedWork W1980714464 @default.
• W1966067794 hasRelatedWork W2022987011 @default.

• next