FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966074310> ?p ?o ?g. }

• W1966074310 endingPage "780" @default.
• W1966074310 startingPage "769" @default.
• W1966074310 abstract "Saccharomyces cerevisiae Sir4p plays important roles in silent chromatin at telomeric and silent mating type loci. The C terminus of Sir4p (Sir4CT) is critical for its functions in vivo because over-expression or deletion of Sir4CT fragments disrupts normal telomeric structure and abolishes the telomere position effect. The 2.5 Å resolution X-ray crystal structure of an Sir4CT fragment (Sir4p 1217–1358) reveals a 72 residue homodimeric, parallel coiled coil, burying an extensive 3600 Å2 of surface area. The crystal structure is consistent with results of protein cross-linking and analytical ultracentrifugation results demonstrating that Sir4CT exists as a dimer in solution. Disruption of the coiled coil in vivo by point mutagenesis results in total derepression of telomeric and HML silent mating marker genes, suggesting that coiled coil dimerization is essential for Sir4p-mediated silencing. In addition to the coiled coil dimerization interface (Sir4CC interface), a crystallographic interface between pairs of coiled coils is significantly hydrophobic and buries 1228 Å2 of surface area (interface II). Remarkably, interface II mutants are deficient in telomeric silencing but not in mating type silencing in vivo. However, point mutants of interface II do not affect the oligomerization state of Sir4CT in solution. These results are consistent with the hypothesis that interface II mimics a protein interface between Sir4p and one of its protein partners that is essential for telomeric silencing but not mating type silencing." @default.
• W1966074310 created "2016-06-24" @default.
• W1966074310 creator A5020514301 @default.
• W1966074310 creator A5022888057 @default.
• W1966074310 creator A5024579115 @default.
• W1966074310 creator A5078143614 @default.
• W1966074310 creator A5082938118 @default.
• W1966074310 creator A5088238437 @default.
• W1966074310 date "2003-12-01" @default.
• W1966074310 modified "2023-10-18" @default.
• W1966074310 title "The Sir4 C-terminal Coiled Coil is Required for Telomeric and Mating Type Silencing in Saccharomyces cerevisiae" @default.
• W1966074310 cites W1536757970 @default.
• W1966074310 cites W1827970445 @default.
• W1966074310 cites W1965231243 @default.
• W1966074310 cites W1965277349 @default.
• W1966074310 cites W1971227936 @default.
• W1966074310 cites W1973570145 @default.
• W1966074310 cites W1975639729 @default.
• W1966074310 cites W1975685790 @default.
• W1966074310 cites W1976091857 @default.
• W1966074310 cites W1986191025 @default.
• W1966074310 cites W1986459831 @default.
• W1966074310 cites W1989247292 @default.
• W1966074310 cites W1990186602 @default.
• W1966074310 cites W1994369498 @default.
• W1966074310 cites W1995017064 @default.
• W1966074310 cites W1995111407 @default.
• W1966074310 cites W1998130142 @default.
• W1966074310 cites W1998377570 @default.
• W1966074310 cites W2000737623 @default.
• W1966074310 cites W2004010498 @default.
• W1966074310 cites W2006901859 @default.
• W1966074310 cites W2007067931 @default.
• W1966074310 cites W2013083986 @default.
• W1966074310 cites W2015959474 @default.
• W1966074310 cites W2021432006 @default.
• W1966074310 cites W2024645073 @default.
• W1966074310 cites W2025120281 @default.
• W1966074310 cites W2032155561 @default.
• W1966074310 cites W2034564804 @default.
• W1966074310 cites W2036347938 @default.
• W1966074310 cites W2044347050 @default.
• W1966074310 cites W2046351190 @default.
• W1966074310 cites W2058346978 @default.
• W1966074310 cites W2059229862 @default.
• W1966074310 cites W2060599714 @default.
• W1966074310 cites W2061769846 @default.
• W1966074310 cites W2063989075 @default.
• W1966074310 cites W2070614630 @default.
• W1966074310 cites W2094051937 @default.
• W1966074310 cites W2095018331 @default.
• W1966074310 cites W2097160587 @default.
• W1966074310 cites W2098812571 @default.
• W1966074310 cites W2100784948 @default.
• W1966074310 cites W2102536188 @default.
• W1966074310 cites W2106795964 @default.
• W1966074310 cites W2111217339 @default.
• W1966074310 cites W2114217399 @default.
• W1966074310 cites W2116875438 @default.
• W1966074310 cites W2138292935 @default.
• W1966074310 cites W2149752144 @default.
• W1966074310 cites W2165499430 @default.
• W1966074310 cites W2168955594 @default.
• W1966074310 cites W4240065971 @default.
• W1966074310 cites W955649814 @default.
• W1966074310 doi "https://doi.org/10.1016/j.jmb.2003.09.066" @default.
• W1966074310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14636601" @default.
• W1966074310 hasPublicationYear "2003" @default.
• W1966074310 type Work @default.
• W1966074310 sameAs 1966074310 @default.
• W1966074310 citedByCount "32" @default.
• W1966074310 countsByYear W19660743102012 @default.
• W1966074310 countsByYear W19660743102013 @default.
• W1966074310 countsByYear W19660743102014 @default.
• W1966074310 countsByYear W19660743102015 @default.
• W1966074310 countsByYear W19660743102016 @default.
• W1966074310 countsByYear W19660743102017 @default.
• W1966074310 countsByYear W19660743102018 @default.
• W1966074310 countsByYear W19660743102019 @default.
• W1966074310 countsByYear W19660743102022 @default.
• W1966074310 crossrefType "journal-article" @default.
• W1966074310 hasAuthorship W1966074310A5020514301 @default.
• W1966074310 hasAuthorship W1966074310A5022888057 @default.
• W1966074310 hasAuthorship W1966074310A5024579115 @default.
• W1966074310 hasAuthorship W1966074310A5078143614 @default.
• W1966074310 hasAuthorship W1966074310A5082938118 @default.
• W1966074310 hasAuthorship W1966074310A5088238437 @default.
• W1966074310 hasConcept C104317684 @default.
• W1966074310 hasConcept C119056186 @default.
• W1966074310 hasConcept C12554922 @default.
• W1966074310 hasConcept C143065580 @default.
• W1966074310 hasConcept C150194340 @default.
• W1966074310 hasConcept C186310378 @default.
• W1966074310 hasConcept C198348125 @default.
• W1966074310 hasConcept C2777576037 @default.
• W1966074310 hasConcept C2779222958 @default.
• W1966074310 hasConcept C54355233 @default.
• W1966074310 hasConcept C55988834 @default.

• next