Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966075174> ?p ?o ?g. }
- W1966075174 endingPage "23" @default.
- W1966075174 startingPage "1" @default.
- W1966075174 abstract "This study was designed to identify and validate potential new biomarkers for prostate cancer and to distinguish patients with and without biochemical relapse. Prostate tissue samples analyzed by 2D-DIGE (two-dimensional difference in gel electrophoresis) and mass spectrometry (MS) revealed downregulation of secernin-1 (P<0.044) in prostate cancer, while vinculin showed significant upregulation (P<0.001). Secernin-1 overexpression in prostate tissue was validated using Western blot and immunohistochemistry while vinculin expression was validated using immunohistochemistry. These findings indicate that secernin-1 and vinculin are potential new tissue biomarkers for prostate cancer diagnosis and prognosis, respectively. For validation, protein levels in urine were also examined by Western blot analysis. Urinary vinculin levels in prostate cancer patients were significantly higher than in urine from nontumor patients (P=0.006). Using multiple reaction monitoring-MS (MRM-MS) analysis, prostatic acid phosphatase (PAP) showed significant higher levels in the urine of prostate cancer patients compared to controls (P=0.012), while galectin-3 showed significant lower levels in the urine of prostate cancer patients with biochemical relapse, compared to those without relapse (P=0.017). Three proteins were successfully differentiated between patients with and without prostate cancer and patients with and without relapse by using MRM. Thus, this technique shows promise for implementation as a noninvasive clinical diagnostic technique." @default.
- W1966075174 created "2016-06-24" @default.
- W1966075174 creator A5017462100 @default.
- W1966075174 creator A5017764283 @default.
- W1966075174 creator A5036843256 @default.
- W1966075174 creator A5038162029 @default.
- W1966075174 creator A5039751847 @default.
- W1966075174 creator A5043258316 @default.
- W1966075174 creator A5043994335 @default.
- W1966075174 creator A5048118430 @default.
- W1966075174 creator A5051457514 @default.
- W1966075174 creator A5062230035 @default.
- W1966075174 creator A5062458606 @default.
- W1966075174 creator A5064651221 @default.
- W1966075174 creator A5075228549 @default.
- W1966075174 creator A5076135264 @default.
- W1966075174 creator A5083037852 @default.
- W1966075174 date "2015-01-01" @default.
- W1966075174 modified "2023-10-16" @default.
- W1966075174 title "Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS" @default.
- W1966075174 cites W145802773 @default.
- W1966075174 cites W163529261 @default.
- W1966075174 cites W1776891179 @default.
- W1966075174 cites W1971411781 @default.
- W1966075174 cites W1974500649 @default.
- W1966075174 cites W1976367185 @default.
- W1966075174 cites W1979043529 @default.
- W1966075174 cites W1979542564 @default.
- W1966075174 cites W1985738617 @default.
- W1966075174 cites W1992396342 @default.
- W1966075174 cites W1995151961 @default.
- W1966075174 cites W1995761153 @default.
- W1966075174 cites W1997875975 @default.
- W1966075174 cites W2001670844 @default.
- W1966075174 cites W2002007120 @default.
- W1966075174 cites W2004254720 @default.
- W1966075174 cites W2007050315 @default.
- W1966075174 cites W2007939915 @default.
- W1966075174 cites W2008730491 @default.
- W1966075174 cites W2009683204 @default.
- W1966075174 cites W2010896383 @default.
- W1966075174 cites W2017240209 @default.
- W1966075174 cites W2018391923 @default.
- W1966075174 cites W2018596412 @default.
- W1966075174 cites W2023914797 @default.
- W1966075174 cites W2028666847 @default.
- W1966075174 cites W2030244758 @default.
- W1966075174 cites W2035684081 @default.
- W1966075174 cites W2040999156 @default.
- W1966075174 cites W2042172693 @default.
- W1966075174 cites W2042413159 @default.
- W1966075174 cites W2043394879 @default.
- W1966075174 cites W2044931584 @default.
- W1966075174 cites W2046229657 @default.
- W1966075174 cites W2053230833 @default.
- W1966075174 cites W2056927942 @default.
- W1966075174 cites W2060714317 @default.
- W1966075174 cites W2061332484 @default.
- W1966075174 cites W2062189636 @default.
- W1966075174 cites W2064998843 @default.
- W1966075174 cites W2065673022 @default.
- W1966075174 cites W2068376654 @default.
- W1966075174 cites W2069149296 @default.
- W1966075174 cites W2071224786 @default.
- W1966075174 cites W2071879495 @default.
- W1966075174 cites W2072347898 @default.
- W1966075174 cites W2076819344 @default.
- W1966075174 cites W2078208642 @default.
- W1966075174 cites W2080078243 @default.
- W1966075174 cites W2081410039 @default.
- W1966075174 cites W2085382634 @default.
- W1966075174 cites W2089216636 @default.
- W1966075174 cites W2089270457 @default.
- W1966075174 cites W2089800336 @default.
- W1966075174 cites W2092649535 @default.
- W1966075174 cites W2094941740 @default.
- W1966075174 cites W2095761465 @default.
- W1966075174 cites W2103263363 @default.
- W1966075174 cites W2106376891 @default.
- W1966075174 cites W2106787323 @default.
- W1966075174 cites W2108643130 @default.
- W1966075174 cites W2114471425 @default.
- W1966075174 cites W2115163261 @default.
- W1966075174 cites W2117692326 @default.
- W1966075174 cites W2125323026 @default.
- W1966075174 cites W2132528779 @default.
- W1966075174 cites W2140687262 @default.
- W1966075174 cites W2142048338 @default.
- W1966075174 cites W2142912172 @default.
- W1966075174 cites W2145310145 @default.
- W1966075174 cites W2147507661 @default.
- W1966075174 cites W2154300909 @default.
- W1966075174 cites W2163276477 @default.
- W1966075174 cites W2165983722 @default.
- W1966075174 cites W2168203442 @default.
- W1966075174 cites W2168300502 @default.
- W1966075174 cites W2168312698 @default.
- W1966075174 cites W2171715173 @default.