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• W1966084666 abstract "The human malaria parasite Plasmodium falciparum possesses a single gene with high similarity to the metalloproteins arginase and agmatinase. The recombinant protein reveals strict specificity for arginine, and it has been proposed that its function in ornithine production is as a precursor for polyamine biosynthesis. The specific activity of the plasmodial arginase was found to be 31 μmol min-1 mg-1 protein and the kcat was calculated as 96 s-1. The Km value for arginine and Ki value for ornithine were determined as 13 mM and 19 mM, respectively. The active arginase is a homotrimer of ca. 160 kDa. Dialysis of the arginase against EDTA results in monomers of approximately 48 kDa; however, the quaternary structure can be restored by addition of Mn2+. Mutagenic analyses of all the amino acid residues proposed to be involved in metal binding led to complex dissociation, except for the His-193-Ala mutant, which was also inactive but retained the trimeric structure. Substitution of His-233, which has been suggested to be in charge of proton shuttling within the active site, disrupted the trimeric structure and thereby the activity of the Pfarginase. Northern blot analysis identified a stage-specific expression pattern of the plasmodial arginase in the ring/young trophozoite stage, which guarantees the provision of ornithine for essential polyamine biosynthesis." @default.
• W1966084666 created "2016-06-24" @default.
• W1966084666 creator A5021210565 @default.
• W1966084666 creator A5032210073 @default.
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• W1966084666 date "2005-01-01" @default.
• W1966084666 modified "2023-09-27" @default.
• W1966084666 title "Structural metal dependency of the arginase from the human malaria parasite Plasmodium falciparum" @default.
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• W1966084666 doi "https://doi.org/10.1515/bc.2005.015" @default.
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