FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966085730> ?p ?o ?g. }

• W1966085730 endingPage "233" @default.
• W1966085730 startingPage "227" @default.
• W1966085730 abstract "We report the effects of EtOH volume percent (0-70%) on spinach apo- and holocalmodulin that have been site-selectively labeled with fluorescein (F). In these experiments, calmodulin (CaM) has one F reporter group attached to Cys-26, and this site is located immediately adjacent to one of the four primary Ca(2+)-binding sites (EF hands). The optimum analytical CaM-F sensitivity to Ca2+ occurs between approximately 10 and 30% EtOH. Our results also show that added EtOH causes changes in CaM and these changes are surprisingly different for apo- and holo-CaM. Apo-CaM-F appears to lose one of its two waters of hydration at approximately 20% EtOH and retains one water of hydration between approximately 20 and 70% EtOH. In apo-CaM-F, the semiangle that describes the range over which the fluorescein reporter group can precess remains essentially constant (42 +/- 2 degrees) between 0 and 70% EtOH. This shows that the fluorescein reporter group precessional freedom in apo-CaM-F is not affected significantly by EtOH. Holo-CaM-F also appears to lose one water of hydration at approximately 20-30% EtOH but then appears to denature as the EtOH volume percent increases. The fluorescein reporter group semiangle within holo-CaM-F decreases from 43 +/- 1 degrees in neat aqueous buffer to 36 +/- 1 degrees at 70% EtOH. This shows that holo-CaM-F is less nativelike and the EF hand closes down about the fluorescein reporter group in holo-CaM-F as the EtOH volume percent increases." @default.
• W1966085730 created "2016-06-24" @default.
• W1966085730 creator A5036941968 @default.
• W1966085730 creator A5037352164 @default.
• W1966085730 creator A5040703058 @default.
• W1966085730 creator A5057876818 @default.
• W1966085730 date "1999-11-25" @default.
• W1966085730 modified "2023-10-16" @default.
• W1966085730 title "Effects of Ethanol Volume Percent on Fluorescein-Labeled Spinach Apo- and Holocalmodulin" @default.
• W1966085730 cites W1539006605 @default.
• W1966085730 cites W1599390139 @default.
• W1966085730 cites W1965156062 @default.
• W1966085730 cites W1965975136 @default.
• W1966085730 cites W1967525328 @default.
• W1966085730 cites W1971818490 @default.
• W1966085730 cites W1978638679 @default.
• W1966085730 cites W1981413862 @default.
• W1966085730 cites W1984851551 @default.
• W1966085730 cites W1987159249 @default.
• W1966085730 cites W1987442198 @default.
• W1966085730 cites W1988133879 @default.
• W1966085730 cites W1989205567 @default.
• W1966085730 cites W1999214538 @default.
• W1966085730 cites W1999873021 @default.
• W1966085730 cites W2010424836 @default.
• W1966085730 cites W2011185745 @default.
• W1966085730 cites W2012941916 @default.
• W1966085730 cites W2023993152 @default.
• W1966085730 cites W2025061710 @default.
• W1966085730 cites W2027667810 @default.
• W1966085730 cites W2032891852 @default.
• W1966085730 cites W2035263589 @default.
• W1966085730 cites W2044468923 @default.
• W1966085730 cites W2045151519 @default.
• W1966085730 cites W2045916983 @default.
• W1966085730 cites W2052650750 @default.
• W1966085730 cites W2065263524 @default.
• W1966085730 cites W2065730495 @default.
• W1966085730 cites W2076031566 @default.
• W1966085730 cites W2077952902 @default.
• W1966085730 cites W2089104537 @default.
• W1966085730 cites W2091587681 @default.
• W1966085730 cites W2098358122 @default.
• W1966085730 cites W2103098481 @default.
• W1966085730 cites W2112622637 @default.
• W1966085730 cites W2116743364 @default.
• W1966085730 cites W2151046161 @default.
• W1966085730 cites W2154222508 @default.
• W1966085730 cites W2316481669 @default.
• W1966085730 cites W2951423423 @default.
• W1966085730 cites W307350572 @default.
• W1966085730 cites W3917119 @default.
• W1966085730 cites W4241765787 @default.
• W1966085730 cites W4299496276 @default.
• W1966085730 doi "https://doi.org/10.1021/ac990551b" @default.
• W1966085730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10655658" @default.
• W1966085730 hasPublicationYear "1999" @default.
• W1966085730 type Work @default.
• W1966085730 sameAs 1966085730 @default.
• W1966085730 citedByCount "6" @default.
• W1966085730 countsByYear W19660857302013 @default.
• W1966085730 crossrefType "journal-article" @default.
• W1966085730 hasAuthorship W1966085730A5036941968 @default.
• W1966085730 hasAuthorship W1966085730A5037352164 @default.
• W1966085730 hasAuthorship W1966085730A5040703058 @default.
• W1966085730 hasAuthorship W1966085730A5057876818 @default.
• W1966085730 hasConcept C121332964 @default.
• W1966085730 hasConcept C12554922 @default.
• W1966085730 hasConcept C178790620 @default.
• W1966085730 hasConcept C185592680 @default.
• W1966085730 hasConcept C2778015030 @default.
• W1966085730 hasConcept C2780054949 @default.
• W1966085730 hasConcept C29688787 @default.
• W1966085730 hasConcept C43617362 @default.
• W1966085730 hasConcept C519063684 @default.
• W1966085730 hasConcept C55493867 @default.
• W1966085730 hasConcept C62520636 @default.
• W1966085730 hasConcept C71240020 @default.
• W1966085730 hasConcept C86803240 @default.
• W1966085730 hasConcept C91881484 @default.
• W1966085730 hasConceptScore W1966085730C121332964 @default.
• W1966085730 hasConceptScore W1966085730C12554922 @default.
• W1966085730 hasConceptScore W1966085730C178790620 @default.
• W1966085730 hasConceptScore W1966085730C185592680 @default.
• W1966085730 hasConceptScore W1966085730C2778015030 @default.
• W1966085730 hasConceptScore W1966085730C2780054949 @default.
• W1966085730 hasConceptScore W1966085730C29688787 @default.
• W1966085730 hasConceptScore W1966085730C43617362 @default.
• W1966085730 hasConceptScore W1966085730C519063684 @default.
• W1966085730 hasConceptScore W1966085730C55493867 @default.
• W1966085730 hasConceptScore W1966085730C62520636 @default.
• W1966085730 hasConceptScore W1966085730C71240020 @default.
• W1966085730 hasConceptScore W1966085730C86803240 @default.
• W1966085730 hasConceptScore W1966085730C91881484 @default.
• W1966085730 hasIssue "1" @default.
• W1966085730 hasLocation W19660857301 @default.
• W1966085730 hasLocation W19660857302 @default.
• W1966085730 hasOpenAccess W1966085730 @default.

• next