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- W1966092913 abstract "A new method is described to visualize chromosome damage in interphase cells immediately after exposure to mutagenic agents. This method involves the fusion of treated interphase cells with untreated mitotic cells which results in the induction of premature chromosome condensation (PCC). Chinese hamster ovary (CHO) cells were treated with X-rays and chromosome aberrations were scored in G2-PCC and the mitotic chromosomes. The incidence of aberrations was significantly higher in PCC than that observed in the mitotic chromosomes of the treated cells. Post-irradiation incubation for I h before fusion allowed the repair of some of the chromosome damage. Data are also presented which indicate that the extent of radiation damage visualized in PCC is inversely proportional to the degree of chromosome condensation. These results indicate that the PCC method has a greater senstivity in the detection of induced chromosome damage than the standard method of scoring metaphase chromosomes." @default.
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- W1966092913 date "1974-05-01" @default.
- W1966092913 modified "2023-10-16" @default.
- W1966092913 title "Premature chromosome condensation I. Visualization of X-ray-induced chromosome damage in interphase cells" @default.
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- W1966092913 doi "https://doi.org/10.1016/0027-5107(74)90145-6" @default.
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