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- W1966104490 abstract "Reaction centers from Rhodopseudomonas sphaeroides R26 in 0.03% LDAO/0.1 M Tris (initial pH = 8.0) were treated with sodium borohydride. The pH of the reaction center solution was never allowed to exceed 10. Absorption spectra taken at various times show that for approx. 8 h after the first addition of NaBH4, A865 (P-870) and A760 diminish very little (no more than 15% loss each), while A800 diminishes markedly (45% loss) and a new peak (at 715 nm) grows in at approximately the same rate that A800 decays. Separate experiments on the absorption and 1H-NMR spectra of purified bacteriochlorophyll (BChl) and bacteriopheophytin (BPh), and their respective NaBH4-reduction products, reveal that A715 in NaBH4-treated reaction centers most likely results from 2a-deoxy-2a-(hydroxy)BPh a, a BPh reduction product. We conclude that at least part of the BChl contributing to A800 in the reaction center is reduced at the acetyl group by NaBH4, apparently with concomitant pheophytinization; if two molecules of BChl contribute equally to that absorption, one of them is reduced. Thus, it is plausible that, of the 6 bacteriochlorin molecules in the reaction center, only one is so configured that its acetyl group is both accessible to the solvent and reactive. In addition to providing a new geometric marker for reaction centers, the NaBH4-reduction technique should make it possible to decide whether any small spectral change in the 800-nm region can be ruled out as the higher-energy exciton transition of the putative BChl special pair. The technique should be interesting to apply to reaction centers from other organisms, especially Rhodopseudomonas viridis. Because NaBH4 treatment is unlikely to significantly modify protein structure, investigations of the photochemical properties of the modified reaction centers should be highly informative, especially since reversible bleaching of P-870 in NaBH4-treated reaction centers now has been observed (Maroti, P., Wraight, C.A. and Pearlstein, R.M., unpublished results)." @default.
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- W1966104490 date "1984-09-01" @default.
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- W1966104490 title "Relative enrichment of P-870 in photosynthetic reaction centers treated with sodium borohydride" @default.
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- W1966104490 doi "https://doi.org/10.1016/0005-2728(84)90123-3" @default.
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