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• W1966111196 abstract "Abstract The cancer‐testis antigen, NY‐ESO‐1, has been engineered into a bacterial expression plasmid which incorporates a His6‐tag. The plasmid was transfected into E. coli strain BL21 and Master and Working cell banks generated from this expression system. Three 15‐litre fermentations were performed under cGMP (code of Good Manufacturing Practice) conditions and the crude NY‐ESO‐1 tagged protein isolated as solubilised inclusion bodies. A three‐step cGMP chromatography process (immobilised metal affinity, anion exchange, and hydrophobic interaction) was utilised to purify the protein. The purified NY‐ESO‐1 is being used in early stage human cancer vaccine trials in Australia and the U.S.A." @default.
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• W1966111196 date "2005-05-01" @default.
• W1966111196 modified "2023-09-27" @default.
• W1966111196 title "Recombinant NY‐ESO‐1 Cancer Antigen: Production and Purification under cGMP Conditions" @default.
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• W1966111196 doi "https://doi.org/10.1081/pb-200054732" @default.
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