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• W1966131710 abstract "Cerebral metabolic rate for oxygen (CMRO 2 ) and cerebral oxygen extraction fraction (OEF) are some of the most fundamental parameters to characterize the pathophysiologic status of cerebral tissue. Although O-15-labeled gases inhalation method is performed in clinical studies, application of the inhalation method on small animals requires too many intensive procedures. On this basis, the development of a new method to measure CMRO 2 and OEF in small animals is of interest. This study was aimed at developing a method to assess CMRO 2 and OEF using intravenously injectable oxygen (injectable 15 O–O 2 ) for small animals such as rats. Injectable 15 O–O 2 , 72 MBq/mL of radioactivity, was obtained after 15 O–O 2 gas circulation into the artificial lung. OEF after injection of injectable 15 O–O 2 was calculated using the same equation as that applied to the bolus inhalation of 15 O–O 2 gas method. Values of 44 ± 4.5 mL · min −1 · 100 g −1 of CBF and 0.54 ± 0.11 of OEF were obtained (n = 13). This OEF value was well accordance with OEF evaluated by arterial-venous difference of oxygen concentration (0.57 ± 0.13). This method is useful to study the CMRO 2 and OEF in small animals using an animal positron emission tomography system. It may accelerate the basic research of several cerebral perfusion diseases." @default.
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• W1966131710 date "2003-06-01" @default.
• W1966131710 modified "2023-10-16" @default.
• W1966131710 title "Development of Injectable O-15 Oxygen and Estimation of Rat OEF" @default.
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• W1966131710 doi "https://doi.org/10.1097/01.wcb.0000066792.97069.b3" @default.
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