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- W1966160816 abstract "Free fatty acids were determined by high performance liquid chromatography after derivatization with monodansyl cadaverine (MDC) at room temperature for 15 min in the presence of diethyl phosphorocyanidate as a coupling reagent. The fluorescence properties (λex 340 nm, λem 518 nm) of MDC-arachidic acid (C200) were identical to those of dansyl alanine. The MDC derivatives of saturated and unsaturated fatty acids were completely separated within 50 min by gradient elution on a TSK-gel 80tm ODS column. When fluorometrically detected, the detection limits (S/N=3)of fatty acid were below 100 fmol. The calibration curves passed through the origin and gave good linearity in the range from 5 to 200 pmol. The method was successfully applied to the determination of fatty acids in the rabbit plasma. This method would be quite useful for the analysis of various biological samples for thermo-labile carboxylic acids, since it requires no heating procedure." @default.
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- W1966160816 date "1989-12-01" @default.
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- W1966160816 title "Fluorometric Determination of Carboxylic Acids by High Performance Liquid Chromatography after Derivatization with Monodansyl Cadaverine" @default.
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- W1966160816 doi "https://doi.org/10.2116/analsci.5.681" @default.
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