FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966170977> ?p ?o ?g. }

• W1966170977 endingPage "e16042" @default.
• W1966170977 startingPage "e16042" @default.
• W1966170977 abstract "The prevalent de novo biosynthetic pathway of vitamin B6 involves only two enzymes (Pdx1 and Pdx2) that form an ornate multisubunit complex functioning as a glutamine amidotransferase. The synthase subunit, Pdx1, utilizes ribose 5-phosphate and glyceraldehyde 3-phosphate, as well as ammonia derived from the glutaminase activity of Pdx2 to directly form the cofactor vitamer, pyridoxal 5′-phosphate. Given the fact that a single enzyme performs the majority of the chemistry behind this reaction, a complicated mechanism is anticipated. Recently, the individual steps along the reaction co-ordinate are beginning to be unraveled. In particular, the binding of the pentose substrate and the first steps of the reaction have been elucidated but it is not known if the latter part of the chemistry, involving the triose sugar, takes place in the same or a disparate site. Here, we demonstrate through the use of enzyme assays, enzyme kinetics, and mutagenesis studies that indeed a second site is involved in binding the triose sugar and moreover, is the location of the final vitamin product, pyridoxal 5′-phosphate. Furthermore, we show that product release is triggered by the presence of a PLP-dependent enzyme. Finally, we provide evidence that a single arginine residue of the C terminus of Pdx1 is responsible for coordinating co-operativity in this elaborate protein machinery." @default.
• W1966170977 created "2016-06-24" @default.
• W1966170977 creator A5006572309 @default.
• W1966170977 creator A5009365489 @default.
• W1966170977 creator A5067528218 @default.
• W1966170977 date "2011-01-21" @default.
• W1966170977 modified "2023-10-17" @default.
• W1966170977 title "It Takes Two to Tango: Defining an Essential Second Active Site in Pyridoxal 5′-Phosphate Synthase" @default.
• W1966170977 cites W1505899335 @default.
• W1966170977 cites W1963863783 @default.
• W1966170977 cites W1963879925 @default.
• W1966170977 cites W1964867612 @default.
• W1966170977 cites W1967999247 @default.
• W1966170977 cites W1968547228 @default.
• W1966170977 cites W1970691223 @default.
• W1966170977 cites W1976597143 @default.
• W1966170977 cites W1979332372 @default.
• W1966170977 cites W1994066024 @default.
• W1966170977 cites W1997016195 @default.
• W1966170977 cites W2001210598 @default.
• W1966170977 cites W2005956146 @default.
• W1966170977 cites W2029054117 @default.
• W1966170977 cites W2031347822 @default.
• W1966170977 cites W2033280480 @default.
• W1966170977 cites W2040885190 @default.
• W1966170977 cites W2043194010 @default.
• W1966170977 cites W2043775846 @default.
• W1966170977 cites W2046985589 @default.
• W1966170977 cites W2053636413 @default.
• W1966170977 cites W2061393819 @default.
• W1966170977 cites W2075909368 @default.
• W1966170977 cites W2076505067 @default.
• W1966170977 cites W2080416920 @default.
• W1966170977 cites W2081252762 @default.
• W1966170977 cites W2085525114 @default.
• W1966170977 cites W2089480036 @default.
• W1966170977 cites W2094161656 @default.
• W1966170977 cites W2107543053 @default.
• W1966170977 cites W2122500335 @default.
• W1966170977 cites W2130830396 @default.
• W1966170977 cites W2132592088 @default.
• W1966170977 cites W2137387674 @default.
• W1966170977 cites W2146660498 @default.
• W1966170977 cites W2152997175 @default.
• W1966170977 cites W2163027365 @default.
• W1966170977 cites W4235039865 @default.
• W1966170977 cites W4293247451 @default.
• W1966170977 cites W4318471123 @default.
• W1966170977 cites W2025898201 @default.
• W1966170977 doi "https://doi.org/10.1371/journal.pone.0016042" @default.
• W1966170977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3024981" @default.
• W1966170977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21283685" @default.
• W1966170977 hasPublicationYear "2011" @default.
• W1966170977 type Work @default.
• W1966170977 sameAs 1966170977 @default.
• W1966170977 citedByCount "20" @default.
• W1966170977 countsByYear W19661709772012 @default.
• W1966170977 countsByYear W19661709772014 @default.
• W1966170977 countsByYear W19661709772015 @default.
• W1966170977 countsByYear W19661709772016 @default.
• W1966170977 countsByYear W19661709772017 @default.
• W1966170977 countsByYear W19661709772019 @default.
• W1966170977 countsByYear W19661709772020 @default.
• W1966170977 countsByYear W19661709772023 @default.
• W1966170977 crossrefType "journal-article" @default.
• W1966170977 hasAuthorship W1966170977A5006572309 @default.
• W1966170977 hasAuthorship W1966170977A5009365489 @default.
• W1966170977 hasAuthorship W1966170977A5067528218 @default.
• W1966170977 hasBestOaLocation W19661709771 @default.
• W1966170977 hasConcept C112243037 @default.
• W1966170977 hasConcept C181199279 @default.
• W1966170977 hasConcept C185592680 @default.
• W1966170977 hasConcept C197957613 @default.
• W1966170977 hasConcept C2776834122 @default.
• W1966170977 hasConcept C2779349466 @default.
• W1966170977 hasConcept C2779461342 @default.
• W1966170977 hasConcept C2780859938 @default.
• W1966170977 hasConcept C41183919 @default.
• W1966170977 hasConcept C515207424 @default.
• W1966170977 hasConcept C55493867 @default.
• W1966170977 hasConcept C58431802 @default.
• W1966170977 hasConcept C71240020 @default.
• W1966170977 hasConcept C75972863 @default.
• W1966170977 hasConceptScore W1966170977C112243037 @default.
• W1966170977 hasConceptScore W1966170977C181199279 @default.
• W1966170977 hasConceptScore W1966170977C185592680 @default.
• W1966170977 hasConceptScore W1966170977C197957613 @default.
• W1966170977 hasConceptScore W1966170977C2776834122 @default.
• W1966170977 hasConceptScore W1966170977C2779349466 @default.
• W1966170977 hasConceptScore W1966170977C2779461342 @default.
• W1966170977 hasConceptScore W1966170977C2780859938 @default.
• W1966170977 hasConceptScore W1966170977C41183919 @default.
• W1966170977 hasConceptScore W1966170977C515207424 @default.
• W1966170977 hasConceptScore W1966170977C55493867 @default.
• W1966170977 hasConceptScore W1966170977C58431802 @default.
• W1966170977 hasConceptScore W1966170977C71240020 @default.
• W1966170977 hasConceptScore W1966170977C75972863 @default.
• W1966170977 hasIssue "1" @default.

• next