Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966219265> ?p ?o ?g. }
- W1966219265 endingPage "15" @default.
- W1966219265 startingPage "1" @default.
- W1966219265 abstract "Nonspecific inflammation in the transplant microenvironment results in β -cell dysfunction and death influencing negatively graft outcome. MicroRNA (miRNA) expression and gene target regulation in transplanted islets are not yet well characterized. We evaluated the impact of inflammation on miRNA expression in transplanted rat islets. Islets exposed in vitro to proinflammatory cytokines and explanted syngeneic islet grafts were evaluated by miRNA arrays. A subset of 26 islet miRNAs was affected by inflammation both in vivo and in vitro . Induction of miRNAs was dependent on NF-κB, a pathway linked with cytokine-mediated islet cell death. RT-PCR confirmed expression of 8 miRNAs. The association between these miRNAs and mRNA target-predicting algorithms in genome-wide RNA studies of β -cell inflammation identified 238 potential miRNA gene targets. Several genes were ontologically associated with regulation of insulin signaling and secretion, diabetes, and islet physiology. One of the most activated miRNAs was miR-21. Overexpression of miR-21 in insulin-secreting MIN6 cells downregulated endogenous expression of the tumor suppressor Pdcd4 and of Pclo, a Ca 2+ sensor protein involved in insulin secretion. Bioinformatics identified both as potential targets. The integrated analysis of miRNA and mRNA expression profiles revealed potential targets that may identify molecular targets for therapeutic interventions." @default.
- W1966219265 created "2016-06-24" @default.
- W1966219265 creator A5001606611 @default.
- W1966219265 creator A5013101960 @default.
- W1966219265 creator A5029272859 @default.
- W1966219265 creator A5030748824 @default.
- W1966219265 creator A5038176676 @default.
- W1966219265 creator A5040389255 @default.
- W1966219265 creator A5061358205 @default.
- W1966219265 creator A5064523617 @default.
- W1966219265 creator A5066206412 @default.
- W1966219265 creator A5067227229 @default.
- W1966219265 creator A5070178366 @default.
- W1966219265 creator A5081771688 @default.
- W1966219265 date "2012-01-01" @default.
- W1966219265 modified "2023-09-30" @default.
- W1966219265 title "Inflammation-Mediated Regulation of MicroRNA Expression in Transplanted Pancreatic Islets" @default.
- W1966219265 cites W144423133 @default.
- W1966219265 cites W1965456684 @default.
- W1966219265 cites W1966607967 @default.
- W1966219265 cites W1969904004 @default.
- W1966219265 cites W1971250081 @default.
- W1966219265 cites W1974976948 @default.
- W1966219265 cites W1978337436 @default.
- W1966219265 cites W1982353113 @default.
- W1966219265 cites W1985149672 @default.
- W1966219265 cites W1988925753 @default.
- W1966219265 cites W1990475883 @default.
- W1966219265 cites W1995641125 @default.
- W1966219265 cites W2000728799 @default.
- W1966219265 cites W2007043238 @default.
- W1966219265 cites W2009326616 @default.
- W1966219265 cites W2009661936 @default.
- W1966219265 cites W2011872309 @default.
- W1966219265 cites W2013937244 @default.
- W1966219265 cites W2014946489 @default.
- W1966219265 cites W2018011543 @default.
- W1966219265 cites W2018511990 @default.
- W1966219265 cites W2018520389 @default.
- W1966219265 cites W2024360391 @default.
- W1966219265 cites W2024915434 @default.
- W1966219265 cites W2025182926 @default.
- W1966219265 cites W2027239352 @default.
- W1966219265 cites W2028060777 @default.
- W1966219265 cites W2029226240 @default.
- W1966219265 cites W2033045615 @default.
- W1966219265 cites W2033521677 @default.
- W1966219265 cites W2036258428 @default.
- W1966219265 cites W2037130549 @default.
- W1966219265 cites W2041028158 @default.
- W1966219265 cites W2044839499 @default.
- W1966219265 cites W2048135758 @default.
- W1966219265 cites W2049481764 @default.
- W1966219265 cites W2049513724 @default.
- W1966219265 cites W2049772720 @default.
- W1966219265 cites W2057309084 @default.
- W1966219265 cites W2058455617 @default.
- W1966219265 cites W2059073003 @default.
- W1966219265 cites W2061283560 @default.
- W1966219265 cites W2063414930 @default.
- W1966219265 cites W2067066548 @default.
- W1966219265 cites W2069264371 @default.
- W1966219265 cites W2071315808 @default.
- W1966219265 cites W2072738635 @default.
- W1966219265 cites W2076449784 @default.
- W1966219265 cites W2076458350 @default.
- W1966219265 cites W2077032611 @default.
- W1966219265 cites W2082515526 @default.
- W1966219265 cites W2082948150 @default.
- W1966219265 cites W2087400084 @default.
- W1966219265 cites W2088154284 @default.
- W1966219265 cites W2089934054 @default.
- W1966219265 cites W2090038705 @default.
- W1966219265 cites W2090070787 @default.
- W1966219265 cites W2096433394 @default.
- W1966219265 cites W2097588661 @default.
- W1966219265 cites W2106121928 @default.
- W1966219265 cites W2106210346 @default.
- W1966219265 cites W2107478456 @default.
- W1966219265 cites W2108255115 @default.
- W1966219265 cites W2108485972 @default.
- W1966219265 cites W2111298415 @default.
- W1966219265 cites W2111480851 @default.
- W1966219265 cites W2112192177 @default.
- W1966219265 cites W2112766099 @default.
- W1966219265 cites W2116611386 @default.
- W1966219265 cites W2118893606 @default.
- W1966219265 cites W2121079193 @default.
- W1966219265 cites W2121236121 @default.
- W1966219265 cites W2125107535 @default.
- W1966219265 cites W2125352391 @default.
- W1966219265 cites W2125945390 @default.
- W1966219265 cites W2128442314 @default.
- W1966219265 cites W2129644963 @default.
- W1966219265 cites W2131072552 @default.
- W1966219265 cites W2133968430 @default.
- W1966219265 cites W2135414402 @default.
- W1966219265 cites W2137937946 @default.