Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966235939> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1966235939 endingPage "492" @default.
- W1966235939 startingPage "484" @default.
- W1966235939 abstract "Immunoreactive dynorphin (ir-Dyn), immunoreactive leucine-enkephalin (ir-Leu-Enk) and various other neuropeptides were measured in acid extracts of bovine adrenal medulla and isolated adrenal chromaffin cells. Their respective levels ranged as follows: Leu-Enk > Dyn > bombesin > vasoactive intestinal peptide (VIP) > neurotensin > substance P. Comparisons of the total catecholamine levels with the levels of Leu-Enk in both extracts gave ratios in the same order of magnitude (2600, tissue extract and 5000, cell extract). However, the catecholamine/Dyn ratio in the tissue extract (138 000) was much higher than that found in the cell extract (20 180), suggesting a possible selective degradation of Dyn in tissue extract as compared with cell extract or an induction of Dyn biosynthesis in cells which have been isolated from their natural microenvironment. Immunofluorescence staining of isolated chromaffin cell sections revealed the presence of ir-Dyn in 5 to 10% of the total cell population. To localize ir-Dyn in regard to Leu-Enk and catecholamines, adrenal chromaffin cells were separated into three populations (I, II, and III) on a stepwise bovine serum albumin (BSA) gradient. Relative high levels of ir-Dyn were measured in cell layer I (4 pmol/10 6 cells), a cell population enriched in noradrenaline. However, ir-Leu-Enk was more concentrated in cell layers II and III (5.3 and 8.3 pmol/10 6 cells), two populations enriched in adrenaline. Isolation and high pressure liquid chromatography (HPLC) analysis of adrenomedullary Dyn indicated the presence of at least five molecular forms corresponding to Dyn-(1-11), Dyn-(1-12), Dyn-(1-13), Ala-containing-Dyn-(1-13) and a nonidentified molecule eluting closely to Dyn-(1-13). These data indicate that adrenal ir-Dyn and ir-Leu-Enk have distinct cellular distributions. In addition, the identification of Dyn fragments in bovine adrenal medulla indicates that these short peptides may be considered as natural active forms of Dyn." @default.
- W1966235939 created "2016-06-24" @default.
- W1966235939 creator A5010987591 @default.
- W1966235939 creator A5015273914 @default.
- W1966235939 creator A5062478724 @default.
- W1966235939 creator A5073616760 @default.
- W1966235939 creator A5078845932 @default.
- W1966235939 date "1984-05-01" @default.
- W1966235939 modified "2023-10-15" @default.
- W1966235939 title "Dynorphin and enkephalins in adrenal paraneurones. Opiates in the adrenal medulla" @default.
- W1966235939 cites W1489355446 @default.
- W1966235939 cites W2006675106 @default.
- W1966235939 cites W2034184844 @default.
- W1966235939 cites W2069665669 @default.
- W1966235939 cites W2073275161 @default.
- W1966235939 cites W2472949332 @default.
- W1966235939 doi "https://doi.org/10.1139/y84-078" @default.
- W1966235939 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6203634" @default.
- W1966235939 hasPublicationYear "1984" @default.
- W1966235939 type Work @default.
- W1966235939 sameAs 1966235939 @default.
- W1966235939 citedByCount "40" @default.
- W1966235939 countsByYear W19662359392012 @default.
- W1966235939 crossrefType "journal-article" @default.
- W1966235939 hasAuthorship W1966235939A5010987591 @default.
- W1966235939 hasAuthorship W1966235939A5015273914 @default.
- W1966235939 hasAuthorship W1966235939A5062478724 @default.
- W1966235939 hasAuthorship W1966235939A5073616760 @default.
- W1966235939 hasAuthorship W1966235939A5078845932 @default.
- W1966235939 hasConcept C118303440 @default.
- W1966235939 hasConcept C126322002 @default.
- W1966235939 hasConcept C134018914 @default.
- W1966235939 hasConcept C144024400 @default.
- W1966235939 hasConcept C149923435 @default.
- W1966235939 hasConcept C151077648 @default.
- W1966235939 hasConcept C170493617 @default.
- W1966235939 hasConcept C185592680 @default.
- W1966235939 hasConcept C2775894120 @default.
- W1966235939 hasConcept C2777597313 @default.
- W1966235939 hasConcept C2778448772 @default.
- W1966235939 hasConcept C2780694816 @default.
- W1966235939 hasConcept C2780910648 @default.
- W1966235939 hasConcept C2781063702 @default.
- W1966235939 hasConcept C2781181269 @default.
- W1966235939 hasConcept C2908647359 @default.
- W1966235939 hasConcept C55493867 @default.
- W1966235939 hasConcept C71924100 @default.
- W1966235939 hasConcept C86803240 @default.
- W1966235939 hasConcept C88016717 @default.
- W1966235939 hasConceptScore W1966235939C118303440 @default.
- W1966235939 hasConceptScore W1966235939C126322002 @default.
- W1966235939 hasConceptScore W1966235939C134018914 @default.
- W1966235939 hasConceptScore W1966235939C144024400 @default.
- W1966235939 hasConceptScore W1966235939C149923435 @default.
- W1966235939 hasConceptScore W1966235939C151077648 @default.
- W1966235939 hasConceptScore W1966235939C170493617 @default.
- W1966235939 hasConceptScore W1966235939C185592680 @default.
- W1966235939 hasConceptScore W1966235939C2775894120 @default.
- W1966235939 hasConceptScore W1966235939C2777597313 @default.
- W1966235939 hasConceptScore W1966235939C2778448772 @default.
- W1966235939 hasConceptScore W1966235939C2780694816 @default.
- W1966235939 hasConceptScore W1966235939C2780910648 @default.
- W1966235939 hasConceptScore W1966235939C2781063702 @default.
- W1966235939 hasConceptScore W1966235939C2781181269 @default.
- W1966235939 hasConceptScore W1966235939C2908647359 @default.
- W1966235939 hasConceptScore W1966235939C55493867 @default.
- W1966235939 hasConceptScore W1966235939C71924100 @default.
- W1966235939 hasConceptScore W1966235939C86803240 @default.
- W1966235939 hasConceptScore W1966235939C88016717 @default.
- W1966235939 hasIssue "5" @default.
- W1966235939 hasLocation W19662359391 @default.
- W1966235939 hasLocation W19662359392 @default.
- W1966235939 hasOpenAccess W1966235939 @default.
- W1966235939 hasPrimaryLocation W19662359391 @default.
- W1966235939 hasRelatedWork W1972292508 @default.
- W1966235939 hasRelatedWork W1976445028 @default.
- W1966235939 hasRelatedWork W1978057833 @default.
- W1966235939 hasRelatedWork W1980863532 @default.
- W1966235939 hasRelatedWork W1989352034 @default.
- W1966235939 hasRelatedWork W1994991608 @default.
- W1966235939 hasRelatedWork W2011433107 @default.
- W1966235939 hasRelatedWork W2040003803 @default.
- W1966235939 hasRelatedWork W2109634684 @default.
- W1966235939 hasRelatedWork W2145721166 @default.
- W1966235939 hasVolume "62" @default.
- W1966235939 isParatext "false" @default.
- W1966235939 isRetracted "false" @default.
- W1966235939 magId "1966235939" @default.
- W1966235939 workType "article" @default.