Matches in SemOpenAlex for { <https://semopenalex.org/work/W1966236040> ?p ?o ?g. }
- W1966236040 endingPage "860" @default.
- W1966236040 startingPage "853" @default.
- W1966236040 abstract "Nucleophosmin/B23 (NPM) is a universally expressed nucleolar phosphoprotein that participates in proliferation, apoptosis, ribosome assembly, and centrosome duplication; however, the role of NPM in cell cycle regulation is not well characterized. We investigated the mechanism by which NPM is involved in cell cycle regulation. NPM was knocked down using siRNA in HepG2 hepatoblastoma cells. NPM translocation following actinomycin D (ActD) treatment was investigated using immunofluorescent staining. Expression of NPM and other factors involved in cell cycle regulation was examined by Western blotting. Cell cycle distribution was measured using flow cytometry to detect 5-ethynyl-2'-deoxyuridine (EdU) incorporation. Cell proliferation was quantified by the MTT assay. Knockdown of NPM increased the percentage of HepG2 cells in S phase and led to decreased expression of P53 and P21Cip1/WAF1. S-phase arrest in HepG2 cells was significantly enhanced by ActD treatment. Furthermore, knockdown of NPM abrogated ActD-induced G2/M phase cell cycle arrest. Taken together, these data demonstrate that inhibition of NPM has a significant effect on the cell cycle." @default.
- W1966236040 created "2016-06-24" @default.
- W1966236040 creator A5005461884 @default.
- W1966236040 creator A5035803191 @default.
- W1966236040 creator A5037960195 @default.
- W1966236040 creator A5040358754 @default.
- W1966236040 creator A5072969391 @default.
- W1966236040 creator A5077184567 @default.
- W1966236040 creator A5089478679 @default.
- W1966236040 date "2011-12-05" @default.
- W1966236040 modified "2023-10-16" @default.
- W1966236040 title "Knockdown of nucleophosmin induces S-phase arrest in HepG2 cells" @default.
- W1966236040 cites W1557736354 @default.
- W1966236040 cites W1603855917 @default.
- W1966236040 cites W1965912619 @default.
- W1966236040 cites W1967541372 @default.
- W1966236040 cites W1971292362 @default.
- W1966236040 cites W1972233897 @default.
- W1966236040 cites W1979862132 @default.
- W1966236040 cites W1988339540 @default.
- W1966236040 cites W1990225407 @default.
- W1966236040 cites W1995680338 @default.
- W1966236040 cites W2001866617 @default.
- W1966236040 cites W2009354494 @default.
- W1966236040 cites W2014674316 @default.
- W1966236040 cites W2025481854 @default.
- W1966236040 cites W2025612389 @default.
- W1966236040 cites W2025863850 @default.
- W1966236040 cites W2027731183 @default.
- W1966236040 cites W2037257739 @default.
- W1966236040 cites W2039260633 @default.
- W1966236040 cites W2045334190 @default.
- W1966236040 cites W2045566232 @default.
- W1966236040 cites W2060192379 @default.
- W1966236040 cites W2074503707 @default.
- W1966236040 cites W2079894802 @default.
- W1966236040 cites W2098784640 @default.
- W1966236040 cites W2117812450 @default.
- W1966236040 cites W2130802787 @default.
- W1966236040 cites W2137752053 @default.
- W1966236040 cites W2138935528 @default.
- W1966236040 cites W2147242002 @default.
- W1966236040 cites W2155781667 @default.
- W1966236040 cites W2157784490 @default.
- W1966236040 cites W2166529745 @default.
- W1966236040 cites W2167230823 @default.
- W1966236040 cites W2248331669 @default.
- W1966236040 cites W2339838060 @default.
- W1966236040 cites W2350295463 @default.
- W1966236040 cites W2393652169 @default.
- W1966236040 doi "https://doi.org/10.5732/cjc.011.10362" @default.
- W1966236040 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4013333" @default.
- W1966236040 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22098949" @default.
- W1966236040 hasPublicationYear "2011" @default.
- W1966236040 type Work @default.
- W1966236040 sameAs 1966236040 @default.
- W1966236040 citedByCount "5" @default.
- W1966236040 countsByYear W19662360402012 @default.
- W1966236040 countsByYear W19662360402013 @default.
- W1966236040 countsByYear W19662360402014 @default.
- W1966236040 countsByYear W19662360402015 @default.
- W1966236040 countsByYear W19662360402023 @default.
- W1966236040 crossrefType "journal-article" @default.
- W1966236040 hasAuthorship W1966236040A5005461884 @default.
- W1966236040 hasAuthorship W1966236040A5035803191 @default.
- W1966236040 hasAuthorship W1966236040A5037960195 @default.
- W1966236040 hasAuthorship W1966236040A5040358754 @default.
- W1966236040 hasAuthorship W1966236040A5072969391 @default.
- W1966236040 hasAuthorship W1966236040A5077184567 @default.
- W1966236040 hasAuthorship W1966236040A5089478679 @default.
- W1966236040 hasBestOaLocation W19662360402 @default.
- W1966236040 hasConcept C105696609 @default.
- W1966236040 hasConcept C153911025 @default.
- W1966236040 hasConcept C173396325 @default.
- W1966236040 hasConcept C185592680 @default.
- W1966236040 hasConcept C190283241 @default.
- W1966236040 hasConcept C2778729363 @default.
- W1966236040 hasConcept C2908722627 @default.
- W1966236040 hasConcept C29537977 @default.
- W1966236040 hasConcept C502942594 @default.
- W1966236040 hasConcept C553184892 @default.
- W1966236040 hasConcept C55493867 @default.
- W1966236040 hasConcept C62112901 @default.
- W1966236040 hasConcept C86803240 @default.
- W1966236040 hasConcept C95444343 @default.
- W1966236040 hasConceptScore W1966236040C105696609 @default.
- W1966236040 hasConceptScore W1966236040C153911025 @default.
- W1966236040 hasConceptScore W1966236040C173396325 @default.
- W1966236040 hasConceptScore W1966236040C185592680 @default.
- W1966236040 hasConceptScore W1966236040C190283241 @default.
- W1966236040 hasConceptScore W1966236040C2778729363 @default.
- W1966236040 hasConceptScore W1966236040C2908722627 @default.
- W1966236040 hasConceptScore W1966236040C29537977 @default.
- W1966236040 hasConceptScore W1966236040C502942594 @default.
- W1966236040 hasConceptScore W1966236040C553184892 @default.
- W1966236040 hasConceptScore W1966236040C55493867 @default.
- W1966236040 hasConceptScore W1966236040C62112901 @default.
- W1966236040 hasConceptScore W1966236040C86803240 @default.