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- W1966236316 abstract "We describe a DNA binding assay for isolation of specific sequence(s) recognized by protein of interest directly from genomic or cosmid DNA. In our assay, the protein is fused to the glutathione-S-transferase and bound to glutathione-Sepharose beads. Then the immobilized fusion protein can be used to search for DNA fragment(s) that interact specifically with the protein of interest. As an example of such an approach, we identified and cloned a few prokaryotic oriC regions using the initiator DnaA protein fused to the glutathione-S-transferase." @default.
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- W1966236316 date "1997-06-01" @default.
- W1966236316 modified "2023-10-06" @default.
- W1966236316 title "Glutathione S-transferase fusion proteins as an affinity reagent for rapid isolation of specific sequence directly from genomic DNA" @default.
- W1966236316 doi "https://doi.org/10.1093/nar/25.12.2537" @default.
- W1966236316 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/146755" @default.
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