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- W1966246558 abstract "Fluorescence spectroscopy of intrinsic biomolecules has been extensively used in biology and medicine for the past several decades. In the present study, we report the native fluorescence characteristics of blood plasma from normal human subjects and patients with different liver abnormalities such as hepatitis, leptospirosis, jaundice, cirrhosis and liver cell failure. Native fluorescence spectra of blood plasma -- acetone extract were measured at 405 nm excitation. The average spectrum of normal blood plasma has a prominent emission peak around 464 nm whereas in the case of liver diseased subjects, the primary peak is red shifted with respect to normal. In addition, liver diseased cases show distinct secondary emission peak around 615 nm, which may be attributed to the presence of endogenous porphyrins. The red shift of the prominent emission peak with respect to normal is found to be maximum for hepatitis and minimum for cirrhosis whereas the secondary emission peak around 615 nm was found to be more prominent in the case of cirrhosis than the rest. The ratio parameter I<SUB>465</SUB>/I<SUB>615</SUB> is found to be statistically significant (p less than 0.001) in discriminating liver abnormalities from normal." @default.
- W1966246558 created "2016-06-24" @default.
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- W1966246558 date "1999-05-17" @default.
- W1966246558 modified "2023-10-18" @default.
- W1966246558 title "<title>Native fluorescence characterization of human liver abnormalities</title>" @default.
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- W1966246558 doi "https://doi.org/10.1117/12.348380" @default.
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