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- W1966281083 abstract "IMP pyrophosphorylase was purified 35 times from an extract of beef liver by a modification of the method of Lukens and Herrington. It was found that increasing the temperature of the heating phases gives a better preparation. The kinetic constants of the enzyme was determined, Km (hypoxanthine) = 1.0·10−5 M, Km (5-phosphoribosyl-1-pyrophosphate) = 5.5·10−5 M. The effect of purine analogues was determined by measuring the conversion of [14C]hypoxanthine to [14C]IMP. 41 substances were examined and 4 competitive inhibitors; 6-mercaptopurine, 2-amino-6-mercaptopurine, 4-hydroxy-6-aminopyrazolo (4,3d)pyrimidine and isoguanine, were found." @default.
- W1966281083 created "2016-06-24" @default.
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- W1966281083 date "1973-01-01" @default.
- W1966281083 modified "2023-10-12" @default.
- W1966281083 title "Effect of purine analogues on IMP-pyrophosphorylase" @default.
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- W1966281083 doi "https://doi.org/10.1016/0005-2744(73)90380-x" @default.
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