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- W1966288099 abstract "Abstract The ependymal glial cells (EGCs) from the periventricular zone of the cerebellum were studied to determine their distribution and the functional properties of their γ‐aminobutyric acid type A (GABA A ) receptors. EGCs were identified by the presence of ciliated structures on their ventricular surface and their expression of glial fibrillary acidic protein (GFAP). Interestingly, diverse cell types, including neurons, astrocytes, and other types of glia, were identified in the subventricular zone by their current profiles. Electron microscopy showed ciliated cells and myelinated axons in this zone, but we found no collateral connections to suggest the presence of functional synapses. GABA‐mediated currents were recorded from EGCs in cerebellar slices from postnatal days 13 to 35 (PN13–PN35). These currents were blocked by TPMPA (a highly specific GABA A ρ subunit antagonist) and bicuculline (a selective antagonist for classic GABA A receptors). Pentobarbital failed to modulate GABA A ‐mediated currents despite the expression of GABAα1 and GABAγ2 subunits. In situ hybridization, RT‐PCR, and immunofluorescence studies confirmed GABAρ1 expression in EGCs of the cerebellum. We conclude that cerebellar EGCs express GABAρ1, which is functionally involved in GABA A receptor‐mediated responses that are unique among glial cells of the brain. © 2013 Wiley Periodicals, Inc." @default.
- W1966288099 created "2016-06-24" @default.
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- W1966288099 date "2013-01-29" @default.
- W1966288099 modified "2023-10-17" @default.
- W1966288099 title "γ-Aminobutyric acid-ρ expression in ependymal glial cells of the mouse cerebellum" @default.
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- W1966288099 doi "https://doi.org/10.1002/jnr.23183" @default.
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